##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727275.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8873752
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.343645619124807	31.0	31.0	34.0	30.0	34.0
2	31.575754427214104	31.0	31.0	34.0	30.0	34.0
3	31.72590286498879	31.0	31.0	34.0	30.0	34.0
4	35.52545788974044	37.0	35.0	37.0	33.0	37.0
5	35.37154993738838	37.0	35.0	37.0	33.0	37.0
6	35.38231032375032	37.0	35.0	37.0	33.0	37.0
7	35.2959891148637	37.0	35.0	37.0	32.0	37.0
8	35.301812919720994	37.0	35.0	37.0	32.0	37.0
9	36.8881265782501	39.0	37.0	39.0	33.0	39.0
10	36.62765299278141	39.0	35.0	39.0	32.0	39.0
11	36.78149332999164	39.0	37.0	39.0	32.0	39.0
12	36.63724949716873	39.0	35.0	39.0	32.0	39.0
13	36.69728610851419	39.0	35.0	39.0	32.0	39.0
14	37.73334526364947	39.0	37.0	41.0	32.0	41.0
15	37.72973461507601	39.0	37.0	41.0	32.0	41.0
16	37.67334133295589	39.0	37.0	41.0	32.0	41.0
17	37.66875094097739	39.0	37.0	41.0	32.0	41.0
18	37.60539420078452	39.0	36.0	41.0	32.0	41.0
19	37.615190620607834	39.0	37.0	41.0	32.0	41.0
20	37.54910189060952	39.0	36.0	41.0	32.0	41.0
21	37.446935862079535	39.0	36.0	41.0	32.0	41.0
22	37.36958594290217	39.0	36.0	41.0	31.0	41.0
23	37.253772361454324	39.0	36.0	41.0	31.0	41.0
24	37.111654799457995	39.0	36.0	40.0	31.0	41.0
25	36.992630287616784	39.0	36.0	40.0	31.0	41.0
26	36.78076511491419	39.0	36.0	40.0	30.0	41.0
27	36.62545741643444	39.0	35.0	40.0	30.0	41.0
28	36.3887303814666	38.0	35.0	40.0	30.0	41.0
29	36.323920253800196	38.0	35.0	40.0	30.0	41.0
30	36.22453354567493	38.0	35.0	40.0	30.0	41.0
31	36.37364364025499	38.0	35.0	40.0	30.0	41.0
32	36.33732720950507	38.0	35.0	40.0	30.0	41.0
33	36.31396753030736	38.0	35.0	40.0	30.0	41.0
34	36.35482116245755	38.0	35.0	40.0	30.0	41.0
35	36.138810618101566	38.0	35.0	40.0	29.0	41.0
36	36.10090117461024	38.0	35.0	40.0	29.0	41.0
37	36.023006615465476	38.0	35.0	40.0	29.0	41.0
38	35.917332037226195	38.0	35.0	40.0	28.0	41.0
39	35.83800831936705	38.0	35.0	40.0	28.0	41.0
40	35.63605394876936	38.0	34.0	40.0	27.0	41.0
41	35.557698930508764	38.0	34.0	40.0	27.0	41.0
42	35.34250821974741	38.0	34.0	40.0	26.0	41.0
43	34.97274816785504	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	4.0
14	26.0
15	62.0
16	210.0
17	370.0
18	630.0
19	1282.0
20	2741.0
21	5331.0
22	9894.0
23	17260.0
24	28305.0
25	44065.0
26	66224.0
27	94854.0
28	133019.0
29	180226.0
30	234653.0
31	299278.0
32	375398.0
33	467683.0
34	588447.0
35	734193.0
36	927558.0
37	1217300.0
38	1644661.0
39	1800074.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.08665601653055	18.80408647886486	12.581397361567012	25.52786014303758
2	18.17377812677208	22.37749037836532	33.83195744032513	25.616774054537473
3	20.008086770962272	24.084524787260225	30.300024161144012	25.607364280633494
4	14.22088424377873	15.657176355615979	34.54646918237066	35.575470218234635
5	14.019729196849315	38.06098029334153	33.35110109004624	14.568189419762914
6	34.12076424944037	35.337994570954876	15.774387203969638	14.766853975635108
7	28.33226576537185	32.06527520715026	20.982928078224408	18.619530949253484
8	28.692913662676172	32.67297756349287	19.963629815212325	18.67047895861863
9	28.199852779297867	13.023284851774086	18.96346663733672	39.81339573159133
10	17.30294017682712	26.47949818746343	31.77218610571943	24.44537552999002
11	37.05753778108741	21.764040734967576	20.469120615496127	20.70930086844888
12	21.948111689395873	25.748488350812597	28.770209038972467	23.533190920819063
13	30.706098164564438	18.736223414853153	25.003268065188212	25.554410355394204
14	23.211072385164698	20.571050441797336	24.948635030593596	31.26924214244437
15	26.233401609600985	27.129674122062458	22.27574085910898	24.361183409227575
16	24.726384059414777	25.516951566823142	25.04090716080413	24.715757212957946
17	24.0852685538203	25.68914479467084	25.321442384236114	24.904144267272738
18	24.30183985308582	23.76351063225567	27.281515192220834	24.65313432243768
19	24.52231029219658	25.08971402401149	27.20365635640933	23.1843193273826
20	24.265136100265142	23.55856913738405	26.92631031383343	25.24998444851738
21	25.396112039191536	24.64646296177761	26.92032637378191	23.037098625248937
22	25.525268229267624	23.930621455276192	26.005516043270084	24.538594272186106
23	23.65976646631549	24.188911297047746	26.894903080455705	25.25641915618106
24	23.557554910256677	25.173725837729066	26.849195244581995	24.419524007432255
25	24.550945304759473	23.719019868934808	26.110984395326803	25.619050430978913
26	23.802400607995356	25.17832366737317	27.049245910861607	23.97002981376987
27	25.57303832696699	24.310393168526684	25.92220291935136	24.194365585154962
28	23.55223585243311	25.27252282912572	26.9792078931212	24.19603342531998
29	23.674517836423643	25.14868569687321	27.32672718372116	23.85006928298199
30	23.927263236565548	25.64371868855474	27.161960352283902	23.26705772259581
31	24.291844081286023	25.255483813385815	26.078844664579314	24.37382744074885
32	23.588669144686484	25.198022212024856	26.077244439556118	25.136064203732538
33	23.068066360204792	24.70935631286518	26.91959387641214	25.302983450517885
34	24.405595288216304	24.91552614947995	27.26445363810032	23.414424924203423
35	24.3174589508474	24.657709613701172	27.63128832088163	23.393543114569802
36	23.046756321339608	25.5103478213049	27.150409432222133	24.292486425133358
37	23.69031724123009	25.1110916780185	27.421039037376747	23.777552043374666
38	23.476811161727305	24.302966772116235	27.50581715603501	24.714404910121445
39	23.645894093051055	24.139045129951793	27.48026990161546	24.734790875381687
40	23.85147793177001	24.197182882731003	28.132598251562584	23.8187409339364
41	22.41874688406888	24.85284691300816	28.586014123450827	24.142392079472135
42	23.51937489350615	24.680732569492587	28.373623693788154	23.42626884321311
43	22.654351845758143	23.403719193414467	28.321244497254373	25.620684463573017
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1176.0
1	1283.5
2	1391.0
3	3669.5
4	5948.0
5	5948.0
6	9588.0
7	13228.0
8	12294.5
9	11361.0
10	14690.5
11	18020.0
12	18020.0
13	33238.0
14	48456.0
15	84396.0
16	120336.0
17	106695.0
18	93054.0
19	93054.0
20	96822.0
21	100590.0
22	79388.0
23	58186.0
24	68573.0
25	78960.0
26	78960.0
27	92860.5
28	106761.0
29	120788.0
30	134815.0
31	152081.0
32	169347.0
33	169347.0
34	194188.0
35	219029.0
36	259067.0
37	299105.0
38	341223.0
39	383341.0
40	383341.0
41	429144.5
42	474948.0
43	499978.5
44	525009.0
45	584642.0
46	644275.0
47	644275.0
48	734459.0
49	824643.0
50	824585.0
51	824527.0
52	778991.5
53	733456.0
54	733456.0
55	705310.0
56	677164.0
57	649644.0
58	622124.0
59	578453.5
60	534783.0
61	534783.0
62	450195.5
63	365608.0
64	326760.5
65	287913.0
66	241942.0
67	195971.0
68	195971.0
69	159532.5
70	123094.0
71	102451.5
72	81809.0
73	59054.0
74	36299.0
75	36299.0
76	28248.5
77	20198.0
78	17929.0
79	15660.0
80	12472.0
81	9284.0
82	9284.0
83	7684.5
84	6085.0
85	5471.0
86	4857.0
87	3194.0
88	1531.0
89	1531.0
90	1117.0
91	703.0
92	517.0
93	331.0
94	335.5
95	340.0
96	340.0
97	180.5
98	21.0
99	18.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	8873752.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.866672939274636
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.11562565506436	28.42996549532794
2	11.062687310126647	8.15688949783806
3	3.9136132223985425	4.328456960429632
4	2.010834778762762	2.9653115249426163
5	1.2200080992500397	2.248881978915866
6	0.8081050311199961	1.7875286329729945
7	0.5613475676487447	1.448651202723453
8	0.45721066842892877	1.3484668941853164
9	0.33430739950505556	1.1092321402859076
>10	2.1682539559706795	15.396612633832868
>50	0.1997337153809888	5.0664918660461815
>100	0.1204002883377271	8.547244733660348
>500	0.012911591866829423	3.3640697427359587
>1k	0.01355336861729244	10.08237868340908
>5k	0.0011319969195712043	2.9872452912661718
>10k+	2.753506020578605E-4	2.732572721427477
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	68718	0.7743962193218833	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	48318	0.5445047371168362	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	46601	0.5251555373645782	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20352	0.22935056107044688	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	14789	0.16666005540835488	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	12525	0.14114660855971634	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	10621	0.11969007022057862	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	10226	0.11523874005043189	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	10117	0.11401039830727747	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG	9972	0.11237636571317296	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	9536	0.10746299874055529	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGG	9395	0.10587404290766747	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	9126	0.10284263071584601	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	8987	0.10127621326356652	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.380757091250691E-5
2	0.0	0.0	0.0	0.0	3.380757091250691E-5
3	0.0	0.0	0.0	0.0	3.380757091250691E-5
4	0.0	0.0	0.0	1.1269190304168969E-5	3.380757091250691E-5
5	0.0	1.1269190304168969E-5	0.0	2.2538380608337937E-5	3.380757091250691E-5
6	0.0	1.1269190304168969E-5	0.0	2.2538380608337937E-5	4.5076761216675875E-5
7	0.0	1.1269190304168969E-5	0.0	2.2538380608337937E-5	4.5076761216675875E-5
8	0.0	1.1269190304168969E-5	0.0	2.2538380608337937E-5	4.5076761216675875E-5
9	0.0	1.1269190304168969E-5	0.0	2.2538380608337937E-5	7.888433212918278E-5
10	0.0	1.1269190304168969E-5	0.0	3.380757091250691E-5	7.888433212918278E-5
11	0.0	1.1269190304168969E-5	0.0	4.5076761216675875E-5	7.888433212918278E-5
12	0.0	2.2538380608337937E-5	0.0	4.5076761216675875E-5	1.0142271273752073E-4
13	0.0	2.2538380608337937E-5	0.0	5.634595152084485E-5	1.126919030416897E-4
14	0.0	2.2538380608337937E-5	0.0	7.888433212918278E-5	1.464994739541966E-4
15	0.0	2.2538380608337937E-5	0.0	1.5776866425836557E-4	1.915762351708725E-4
16	0.0	2.2538380608337937E-5	0.0	3.4934489942923803E-4	2.591913769958863E-4
17	0.0	2.2538380608337937E-5	0.0	6.536130376418002E-4	2.591913769958863E-4
18	0.0	2.2538380608337937E-5	0.0	7.6630494068349E-4	2.704605673000553E-4
19	0.0	2.2538380608337937E-5	0.0	0.0011719957916335728	2.8172975760422424E-4
20	0.0	2.2538380608337937E-5	0.0	0.001476263929846135	3.1553732851673114E-4
21	0.0	2.2538380608337937E-5	0.0	0.0023665299638754834	3.268065188209001E-4
22	0.0	2.2538380608337937E-5	0.0	0.0038653322743299563	3.268065188209001E-4
23	0.0	2.2538380608337937E-5	0.0	0.005927594099992878	4.394984218625898E-4
24	0.0	2.2538380608337937E-5	0.0	0.008542046250560079	4.507676121667588E-4
25	0.0	2.2538380608337937E-5	0.0	0.009826733945235342	4.6203680247092774E-4
26	0.0	2.2538380608337937E-5	0.0	0.012767992614623443	4.6203680247092774E-4
27	0.0	2.2538380608337937E-5	0.0	0.024476681340655	4.6203680247092774E-4
28	0.0	2.2538380608337937E-5	0.0	0.04906605458435169	4.6203680247092774E-4
29	0.0	2.2538380608337937E-5	0.0	0.08647976639419266	4.6203680247092774E-4
30	0.0	2.2538380608337937E-5	0.0	0.15000419213879315	4.6203680247092774E-4
31	0.0	2.2538380608337937E-5	0.0	0.3861162673917414	4.6203680247092774E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	3290	0.0	19.399696	5
CAAGACG	3320	0.0	19.335844	4
CTTATAC	4515	0.0	17.823921	37
ACGGACC	3785	0.0	16.61823	8
GACGGAC	3860	0.0	16.151554	7
CGGACCA	3910	0.0	16.086956	9
CGCAAGA	4040	0.0	15.935644	2
AGACGGA	3965	0.0	15.910467	6
GCGCAAG	4115	0.0	15.5552845	1
TACTTAC	1745	0.0	14.842407	31
CCCGTTA	375	0.0	14.800001	22
TCGTTTA	2525	0.0	14.506931	30
GCAAGAC	4735	0.0	14.495248	3
TAATACC	780	0.0	14.467949	4
AGAGCGA	4315	0.0	14.405562	15
CAGAGCG	4360	0.0	14.3417425	14
GAGCGAA	4430	0.0	14.073363	16
ACGAACG	2465	0.0	14.034484	15
CGCTTCG	2270	0.0	14.017621	32
TACCGTC	2655	0.0	14.00565	7
>>END_MODULE