FastQCFastQC Report
Thu 9 Feb 2017
SRR2727273.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727273.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2792489
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT256190.9174252790252709No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT172880.6190892784179275No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT169100.6055529672632551No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100000.35810346970032825No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA39220.14044818081646876No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG35450.12694768000876638No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT35440.12691186966179635No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC32940.11795928291928814No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31820.11394852405864446No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTA451.323874E-424.6666686
CTTATAC25850.017.67698137
AAGACGG8500.017.6294125
CAAGACG8850.017.3502854
GTATAGA2450.016.6122441
CGCGTAT700.002593738515.8571427
CGCTTCG6800.015.23529432
GTAAACG7200.014.90277927
TACGCGT750.0041063614.85
TTCGGGC7100.014.5915535
TTAGTAC1152.2119704E-514.4782613
CTAGCGG5400.014.38888829
TCTAGCG5450.014.25688128
ACTCTAA8200.014.21341410
TAAACGC7450.014.15436228
ATACCGA1051.6569685E-414.0952396
TTTTACG1051.6569685E-414.0952392
ACGCTTC7500.014.06000131
AGACGGA10150.014.0344836
GACGGAC10150.014.0344837
Produced by FastQC (version 0.11.5)