##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727273.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2792489
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.310010173719576	31.0	31.0	34.0	30.0	34.0
2	31.53942450623798	31.0	31.0	34.0	30.0	34.0
3	31.68941077296992	31.0	31.0	34.0	30.0	34.0
4	35.49116361783341	37.0	35.0	37.0	33.0	37.0
5	35.33247078144265	37.0	35.0	37.0	33.0	37.0
6	35.35298760353219	37.0	35.0	37.0	33.0	37.0
7	35.2566069194901	37.0	35.0	37.0	32.0	37.0
8	35.27977012622073	37.0	35.0	37.0	32.0	37.0
9	36.85118258299317	39.0	37.0	39.0	33.0	39.0
10	36.59006105306055	39.0	35.0	39.0	32.0	39.0
11	36.752651845719	39.0	35.0	39.0	32.0	39.0
12	36.62151471321821	39.0	35.0	39.0	32.0	39.0
13	36.68657674211071	39.0	35.0	39.0	32.0	39.0
14	37.70824021151024	39.0	37.0	41.0	32.0	41.0
15	37.7078197980368	39.0	37.0	41.0	32.0	41.0
16	37.65074777376025	39.0	36.0	41.0	32.0	41.0
17	37.65737734329482	39.0	36.0	41.0	32.0	41.0
18	37.601439074603334	39.0	36.0	41.0	32.0	41.0
19	37.62153512511598	39.0	37.0	41.0	32.0	41.0
20	37.55698017073657	39.0	36.0	41.0	32.0	41.0
21	37.45970959957228	39.0	36.0	41.0	32.0	41.0
22	37.385329002191234	39.0	36.0	41.0	32.0	41.0
23	37.26617222126927	39.0	36.0	41.0	31.0	41.0
24	37.131476972693534	39.0	36.0	41.0	31.0	41.0
25	37.006617394016594	39.0	36.0	40.0	31.0	41.0
26	36.78022473857551	39.0	36.0	40.0	30.0	41.0
27	36.61312327461272	39.0	35.0	40.0	30.0	41.0
28	36.37976085134086	38.0	35.0	40.0	30.0	41.0
29	36.302532257065295	38.0	35.0	40.0	30.0	41.0
30	36.190759211585075	38.0	35.0	40.0	30.0	41.0
31	36.290541520485846	38.0	35.0	40.0	30.0	41.0
32	36.23378713398692	38.0	35.0	40.0	30.0	41.0
33	36.1911785507481	38.0	35.0	40.0	30.0	41.0
34	36.21596862154157	38.0	35.0	40.0	30.0	41.0
35	35.983088205539936	38.0	35.0	40.0	29.0	41.0
36	35.928593093831346	38.0	35.0	40.0	29.0	41.0
37	35.823156689247476	38.0	35.0	40.0	28.0	41.0
38	35.70715766472133	38.0	34.0	40.0	27.0	41.0
39	35.60159341719878	38.0	34.0	40.0	27.0	41.0
40	35.37088203391311	38.0	34.0	40.0	26.0	41.0
41	35.26478850946235	38.0	34.0	40.0	26.0	41.0
42	35.02596894741573	38.0	33.0	40.0	25.0	41.0
43	34.642645682758285	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	2.0
14	6.0
15	14.0
16	66.0
17	116.0
18	206.0
19	447.0
20	828.0
21	1743.0
22	3230.0
23	5697.0
24	9241.0
25	14702.0
26	21464.0
27	31057.0
28	43173.0
29	58834.0
30	76493.0
31	96889.0
32	120542.0
33	150653.0
34	188363.0
35	232901.0
36	294630.0
37	388978.0
38	510040.0
39	542173.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.86527180590505	19.466970147420458	13.146837821026331	24.520920225648158
2	17.820947548942897	23.21377094054802	34.36342990070865	24.60185160980043
3	20.351127614110563	24.996947167920805	30.201050030993855	24.450875186974773
4	14.539144111221209	16.026455251927583	34.08006262513478	35.35433801171643
5	13.944620730824722	38.60477158549237	32.94251114328472	14.508096540398189
6	33.66319437605662	34.98248336877961	16.214280521785405	15.140041733378359
7	27.697011519114312	32.272821844598134	21.560550462329484	18.46961617395807
8	28.31907305633075	33.130336413142544	20.23782367629738	18.312766854229327
9	28.0717668001557	13.499390686946306	19.890857224504735	38.53798528839326
10	17.38921800587218	27.39190736292963	31.891370028673343	23.327504602524847
11	35.508537365769385	22.92603480264381	20.68351925468641	20.881908576900393
12	21.44738976590418	26.615861333742046	29.214152678846723	22.72259622150705
13	30.105543835624776	19.65794672781164	25.414997158448966	24.821512278114614
14	22.82157602053222	21.58887644678278	25.88715658324885	29.702390949436147
15	25.719564159429098	27.7617924367831	23.067557293869374	23.45108610991843
16	23.6621522949598	26.35698833549568	25.910218446697552	24.070640922846962
17	23.382437674776874	26.84669483031088	26.093209319714422	23.677658175197823
18	23.20546294005097	24.80321319081293	28.268544656756035	23.722779212380065
19	23.47300204226409	26.24701475995071	28.311946797283717	21.96803640050149
20	23.284317324078984	24.94777956153095	27.992590123004963	23.775312991385107
21	24.199629792633022	25.991257261890738	27.85217775253546	21.956935192940776
22	24.435405117083718	25.306742479558558	26.762934428747975	23.49491797460975
23	22.620429301601547	25.804076578278373	27.70492560579469	23.87056851432539
24	22.639480406189605	26.757025721497918	27.492964162079065	23.110529710233415
25	23.41280484900746	25.483072628039	26.814214845609058	24.289907677344477
26	22.838693366383897	26.643471111255945	27.745570349605675	22.772265172754487
27	24.091661596518374	26.121606924861656	26.68096454453357	23.1057669340864
28	22.52889805474614	26.993696304622866	27.72100445158423	22.75640118904676
29	22.73466430843595	26.802934586313498	27.955383172503094	22.507017932747452
30	22.847180418615796	27.268683959005745	27.650708740482056	22.2334268818964
31	23.201237319108508	26.849022502863935	26.876596470030854	23.073143707996703
32	22.234895106122174	26.953660336710367	27.062846084622	23.74859847254546
33	21.91672017329343	26.513193068978964	27.73937515957986	23.830711598147747
34	22.971585563989688	26.62843076552853	27.917531635755772	22.482452034726013
35	22.89530952494352	26.520211896985092	28.16401425395051	22.420464324120886
36	21.983112556575872	26.948897560563356	27.83935764832019	23.228632234540584
37	22.22218243294781	26.60579862624347	28.193486169506844	22.978532771301875
38	22.1234891166984	25.77610869729478	28.497014670424843	23.60338751558198
39	22.14572734216679	25.517916095640842	28.61389964293503	23.722456919257336
40	22.311314386556223	25.74148009177476	29.158252727226497	22.788952794442523
41	21.06436229471271	26.10961045862669	29.574762872834953	23.251264373825645
42	21.815162029286416	26.195483670660835	29.34722392818736	22.642130371865385
43	20.953206977717727	25.206652559777314	29.262317595521413	24.57782286698354
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2262.0
1	2051.5
2	1841.0
3	3042.5
4	4244.0
5	4244.0
6	6129.5
7	8015.0
8	7554.5
9	7094.0
10	9512.0
11	11930.0
12	11930.0
13	20541.5
14	29153.0
15	42477.0
16	55801.0
17	48978.0
18	42155.0
19	42155.0
20	43198.5
21	44242.0
22	35550.0
23	26858.0
24	30811.5
25	34765.0
26	34765.0
27	38775.0
28	42785.0
29	46900.0
30	51015.0
31	55384.0
32	59753.0
33	59753.0
34	68198.0
35	76643.0
36	89145.0
37	101647.0
38	113980.0
39	126313.0
40	126313.0
41	139118.5
42	151924.0
43	160719.0
44	169514.0
45	186157.5
46	202801.0
47	202801.0
48	226204.5
49	249608.0
50	247898.0
51	246188.0
52	229490.0
53	212792.0
54	212792.0
55	201921.5
56	191051.0
57	182729.5
58	174408.0
59	160889.0
60	147370.0
61	147370.0
62	124521.0
63	101672.0
64	90073.5
65	78475.0
66	65660.5
67	52846.0
68	52846.0
69	43556.0
70	34266.0
71	28901.5
72	23537.0
73	17439.5
74	11342.0
75	11342.0
76	8822.5
77	6303.0
78	5559.5
79	4816.0
80	3842.0
81	2868.0
82	2868.0
83	2420.5
84	1973.0
85	1678.0
86	1383.0
87	932.5
88	482.0
89	482.0
90	328.0
91	174.0
92	131.5
93	89.0
94	87.0
95	85.0
96	85.0
97	43.5
98	2.0
99	3.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2792489.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.55088847705341
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.00811974935226	41.96134204139402
2	8.773440778751192	8.870104527333684
3	2.9250338991922478	4.43589187289004
4	1.4674595699501993	2.967255402605492
5	0.862038074658293	2.1788395287512605
6	0.577960292325566	1.752984376890893
7	0.40214365178320566	1.4230103225133481
8	0.3084235680520836	1.2472868313836618
9	0.22563608942074345	1.026549431343581
>10	1.2618969921337917	11.44265411531052
>50	0.09502866265974264	3.317482144588303
>100	0.07402929077554128	7.849887305474927
>500	0.013166801041019173	4.483941447908882
>1k	0.005337892313920942	4.530892772542224
>5k	7.11718975189459E-5	0.35978026541805086
>10k+	2.1351569255683767E-4	2.152097613651155
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	25619	0.9174252790252709	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	17288	0.6190892784179275	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16910	0.6055529672632551	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10000	0.35810346970032825	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	3922	0.14044818081646876	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	3545	0.12694768000876638	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	3544	0.12691186966179635	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	3294	0.11795928291928814	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3182	0.11394852405864446	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.581034697003283E-5
2	0.0	0.0	0.0	0.0	3.581034697003283E-5
3	0.0	0.0	0.0	0.0	3.581034697003283E-5
4	0.0	0.0	0.0	0.0	7.162069394006566E-5
5	0.0	0.0	0.0	0.0	7.162069394006566E-5
6	0.0	0.0	0.0	0.0	7.162069394006566E-5
7	0.0	0.0	0.0	0.0	7.162069394006566E-5
8	0.0	0.0	0.0	0.0	7.162069394006566E-5
9	0.0	0.0	0.0	0.0	1.0743104091009848E-4
10	0.0	0.0	0.0	3.581034697003283E-5	1.0743104091009848E-4
11	0.0	0.0	0.0	3.581034697003283E-5	1.0743104091009848E-4
12	0.0	0.0	0.0	1.432413878801313E-4	1.432413878801313E-4
13	0.0	0.0	0.0	2.1486208182019696E-4	1.7905173485016415E-4
14	0.0	0.0	0.0	3.581034697003283E-4	1.7905173485016415E-4
15	0.0	0.0	0.0	6.803965924306238E-4	2.1486208182019696E-4
16	0.0	0.0	0.0	0.0012891724909211818	2.864827757602626E-4
17	0.0	0.0	0.0	0.0022202415121420353	2.864827757602626E-4
18	0.0	0.0	0.0	0.0026141553288123965	2.864827757602626E-4
19	0.0	0.0	0.0	0.0032229312273029543	2.864827757602626E-4
20	0.0	0.0	0.0	0.004046569207613709	2.864827757602626E-4
21	0.0	0.0	0.0	0.0056938451682352195	2.864827757602626E-4
22	0.0	0.0	0.0	0.008701914313717977	2.864827757602626E-4
23	0.0	0.0	0.0	0.014037656012252868	3.2229312273029546E-4
24	0.0	0.0	0.0	0.021486208182019698	3.2229312273029546E-4
25	0.0	0.0	0.0	0.024637518715382584	3.2229312273029546E-4
26	0.0	0.0	0.0	0.03201445019120935	3.2229312273029546E-4
27	0.0	0.0	0.0	0.06463767628090926	3.2229312273029546E-4
28	0.0	0.0	0.0	0.12744902486634682	3.581034697003283E-4
29	0.0	0.0	0.0	0.2054081502201083	3.581034697003283E-4
30	0.0	0.0	0.0	0.3351132269455672	3.581034697003283E-4
31	0.0	0.0	0.0	0.7376215268887362	3.581034697003283E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGTA	45	1.323874E-4	24.666668	6
CTTATAC	2585	0.0	17.676981	37
AAGACGG	850	0.0	17.629412	5
CAAGACG	885	0.0	17.350285	4
GTATAGA	245	0.0	16.612244	1
CGCGTAT	70	0.0025937385	15.857142	7
CGCTTCG	680	0.0	15.235294	32
GTAAACG	720	0.0	14.902779	27
TACGCGT	75	0.00410636	14.8	5
TTCGGGC	710	0.0	14.59155	35
TTAGTAC	115	2.2119704E-5	14.478261	3
CTAGCGG	540	0.0	14.388888	29
TCTAGCG	545	0.0	14.256881	28
ACTCTAA	820	0.0	14.213414	10
TAAACGC	745	0.0	14.154362	28
ATACCGA	105	1.6569685E-4	14.095239	6
TTTTACG	105	1.6569685E-4	14.095239	2
ACGCTTC	750	0.0	14.060001	31
AGACGGA	1015	0.0	14.034483	6
GACGGAC	1015	0.0	14.034483	7
>>END_MODULE