FastQCFastQC Report
Thu 9 Feb 2017
SRR2727272.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727272.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3262282
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT396341.2149164296648787No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT258640.7928192596470814No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT244900.7507015028130616No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140950.4320595215251165No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA54120.16589614263880315No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT39020.11960952486633589No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33280.10201447943494768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACG7000.018.7642844
CTTATAC16300.018.537
AAGACGG6700.017.1194045
ACGGACC7300.015.9657548
CGACTAT700.002593863315.85714336
TCGTTAC9350.015.03743323
GGGCGTC9600.014.64583311
TCTTATA26650.014.43902537
TACGGAT13200.014.43560627
ACGGATG13750.014.12727328
GGCGTCA10000.014.06000112
CGTTACG7400.014.024
AGACGGA8750.013.9542866
GAACAGT20950.013.9522671
TACTTAC10700.013.83177631
TAGTACT1753.5724952E-813.7428574
AGGGCGT10450.013.63157810
AACAGTG21450.013.6270392
GACGGAC8150.013.6196327
ACAGTGG23150.013.50543
Produced by FastQC (version 0.11.5)