##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727272.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3262282
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.108908733211905	31.0	31.0	33.0	30.0	34.0
2	31.32838240225707	31.0	31.0	34.0	30.0	34.0
3	31.467810569411228	31.0	31.0	34.0	30.0	34.0
4	35.29927792876275	37.0	35.0	37.0	33.0	37.0
5	35.12736881728802	37.0	35.0	37.0	32.0	37.0
6	35.14637759703177	37.0	35.0	37.0	32.0	37.0
7	35.05178890114343	37.0	35.0	37.0	32.0	37.0
8	35.07549132784965	37.0	35.0	37.0	32.0	37.0
9	36.58681223756867	39.0	35.0	39.0	32.0	39.0
10	36.32592890498124	38.0	35.0	39.0	32.0	39.0
11	36.49907580031401	38.0	35.0	39.0	32.0	39.0
12	36.36152791205665	38.0	35.0	39.0	32.0	39.0
13	36.429246766527235	38.0	35.0	39.0	32.0	39.0
14	37.365094740430166	39.0	36.0	41.0	32.0	41.0
15	37.37883358949349	39.0	36.0	41.0	32.0	41.0
16	37.314229425904934	39.0	36.0	41.0	32.0	41.0
17	37.34748896631254	39.0	36.0	41.0	32.0	41.0
18	37.3024410519998	39.0	36.0	41.0	32.0	41.0
19	37.34273186683432	39.0	36.0	41.0	31.0	41.0
20	37.279241647411226	39.0	36.0	40.0	31.0	41.0
21	37.186867045828656	39.0	36.0	40.0	31.0	41.0
22	37.12015668786451	39.0	36.0	40.0	31.0	41.0
23	37.00507834699759	39.0	36.0	40.0	31.0	41.0
24	36.863379070233655	39.0	36.0	40.0	30.0	41.0
25	36.73828136255541	39.0	35.0	40.0	30.0	41.0
26	36.494587531059544	38.0	35.0	40.0	30.0	41.0
27	36.3194362106035	38.0	35.0	40.0	30.0	41.0
28	36.08831333404041	38.0	35.0	40.0	29.0	41.0
29	36.0120688524168	38.0	35.0	40.0	29.0	41.0
30	35.90143555952551	38.0	34.0	40.0	29.0	41.0
31	35.94460840601763	38.0	34.0	40.0	29.0	41.0
32	35.88094162307244	38.0	34.0	40.0	29.0	41.0
33	35.803200642985495	38.0	34.0	40.0	29.0	41.0
34	35.826030061165774	38.0	34.0	40.0	29.0	41.0
35	35.58520967837851	38.0	34.0	40.0	27.0	41.0
36	35.5079885797733	38.0	34.0	40.0	27.0	41.0
37	35.398848413472535	38.0	34.0	40.0	26.0	41.0
38	35.26891789244461	38.0	34.0	40.0	26.0	41.0
39	35.12564088573581	38.0	33.0	40.0	25.0	41.0
40	34.89045183708827	38.0	33.0	40.0	25.0	41.0
41	34.766111574658474	38.0	33.0	40.0	24.0	41.0
42	34.496671041927094	38.0	33.0	40.0	23.0	41.0
43	34.12123200875951	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	3.0
14	4.0
15	16.0
16	53.0
17	116.0
18	270.0
19	624.0
20	1307.0
21	2616.0
22	4777.0
23	8359.0
24	13713.0
25	21178.0
26	30996.0
27	44303.0
28	61390.0
29	80959.0
30	102963.0
31	127388.0
32	156526.0
33	189532.0
34	231169.0
35	278260.0
36	343153.0
37	442710.0
38	564396.0
39	555498.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.82689234100547	19.806442238899027	13.37931546077255	23.98734995932295
2	18.007885277851514	24.19913422567393	33.60451365026077	24.188466846213785
3	20.816685988519694	25.55373815016605	30.738605675413716	22.890970185900546
4	14.811472460075493	16.12487822941119	33.786748049371575	35.27690126114174
5	13.54321913310989	39.937626483547405	32.39204949173615	14.127104891606551
6	33.22713977516352	34.86372422739665	16.447964952140865	15.461171045298967
7	27.073410575787133	33.3951510016608	21.1941518237847	18.337286598767367
8	29.28036264185622	32.644878646297286	20.167416550745767	17.907342161100726
9	28.96588952150673	12.904954262077894	19.921974862994677	38.20718135342071
10	17.20320315656341	28.43880449329641	31.749646413154963	22.608345936985213
11	35.43675868609765	23.248817852043445	20.27378381145468	21.040639650404227
12	21.28920798385915	27.24053285399607	28.97373678915557	22.496522372989215
13	30.559865762677784	19.8359921061392	25.47707402364357	24.127068107539447
14	22.91779190149717	21.823619172101	26.43275474039338	28.825834186008443
15	26.160521990434916	28.01229323522614	23.40698934059042	22.420195433748525
16	23.30448440692742	26.54540594589922	26.46049605766761	23.68961358950575
17	23.127675657714448	26.763964611275178	26.42211801432249	23.68624171668789
18	22.272016950098124	25.214466437910644	28.818814559869445	23.694702052121798
19	23.056283914143535	26.175419537612015	29.409290797055558	21.359005751188892
20	22.66002755126626	24.91421649017467	28.94060047537276	23.48515548318631
21	23.460081010777117	26.047257717144014	29.318464804698063	21.17419646738081
22	24.165231577159794	25.345570983746963	27.606779548794368	22.882417890298875
23	22.36566305426692	26.115032360783037	28.63339220827629	22.88591237667375
24	21.775033550134538	26.685430628008245	28.654757620585837	22.88477820127138
25	22.88971339694116	25.69419197972462	27.791220991931414	23.624873631402803
26	22.17889195354663	26.845778507192204	28.650036998640825	22.32529254062034
27	23.481844917146955	26.65897675308266	27.59206592195279	22.267112407817596
28	21.71541270803689	27.75517260616955	28.069093965512486	22.46032072028108
29	22.038162243484773	27.42941903857484	28.7182407897294	21.81417792821099
30	22.610828861514733	27.639456061738375	28.1428460200559	21.606869056690993
31	22.47485042678714	27.508504782848327	27.926157211424396	22.09048757894014
32	21.89608378429578	27.639333448181365	27.65260636572804	22.811976401794816
33	21.28080895520375	27.121444436747037	28.39362139753706	23.20412521051215
34	22.28636273626866	27.558745687834467	28.65944758914159	21.495443986755284
35	22.05069947968937	27.28991546408312	28.74423486381619	21.915150192411325
36	21.229648448540008	27.787021477603712	28.51884049263675	22.464489581219528
37	21.596661478069645	27.79462351813853	28.769523909950152	21.839191093841674
38	21.857950968064685	26.88047814382693	28.36860823190638	22.89296265620201
39	21.336322243141456	27.164297874923136	28.885822868777133	22.61355701315827
40	22.086441331558706	26.72377801796411	29.00675048938136	22.18303016109582
41	20.769449115680374	27.53814661025626	29.344305611838585	22.348098662224785
42	21.32795386787531	27.20301310555004	29.200755789965427	22.268277236609222
43	20.450224719996616	26.37699622534165	29.025449056825863	24.14732999783587
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2006.0
1	1868.0
2	1730.0
3	2872.0
4	4014.0
5	4014.0
6	6340.0
7	8666.0
8	8371.5
9	8077.0
10	11190.0
11	14303.0
12	14303.0
13	26724.5
14	39146.0
15	61075.0
16	83004.0
17	71377.0
18	59750.0
19	59750.0
20	62327.5
21	64905.0
22	51910.5
23	38916.0
24	44121.0
25	49326.0
26	49326.0
27	54939.5
28	60553.0
29	64774.0
30	68995.0
31	73406.0
32	77817.0
33	77817.0
34	85062.5
35	92308.0
36	103566.0
37	114824.0
38	126280.5
39	137737.0
40	137737.0
41	153647.5
42	169558.0
43	179474.0
44	189390.0
45	209954.0
46	230518.0
47	230518.0
48	256773.5
49	283029.0
50	282870.0
51	282711.0
52	270482.0
53	258253.0
54	258253.0
55	240581.5
56	222910.0
57	210082.5
58	197255.0
59	178910.5
60	160566.0
61	160566.0
62	136945.5
63	113325.0
64	100257.0
65	87189.0
66	72434.5
67	57680.0
68	57680.0
69	46130.5
70	34581.0
71	28678.5
72	22776.0
73	16442.0
74	10108.0
75	10108.0
76	7945.0
77	5782.0
78	4980.5
79	4179.0
80	3374.5
81	2570.0
82	2570.0
83	2130.0
84	1690.0
85	1515.5
86	1341.0
87	899.5
88	458.0
89	458.0
90	318.0
91	178.0
92	121.5
93	65.0
94	77.0
95	89.0
96	89.0
97	44.5
98	0.0
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3262282.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.735945295072504
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.7386986637702	39.018740479041504
2	9.33513871549442	8.9124334208951
3	3.0694992438749136	4.3957634396663785
4	1.570864620223127	2.999468303077441
5	0.9134629837281792	2.1802509510161037
6	0.6027439094400288	1.7263530172780415
7	0.43174823683661745	1.4426937150413746
8	0.3242881585278566	1.2384161436260448
9	0.25080931440805315	1.077535774086972
>10	1.5427500395652356	13.495536783570227
>50	0.11518102039879516	3.769925304898911
>100	0.0876544734773412	8.528576334347663
>500	0.012064045066844505	3.9711984095205324
>1k	0.0047740071385626555	3.871080023123293
>5k	6.451360998057642E-5	0.16666893464712224
>10k+	2.580544399223057E-4	3.2053589661634194
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	39634	1.2149164296648787	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	25864	0.7928192596470814	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	24490	0.7507015028130616	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14095	0.4320595215251165	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	5412	0.16589614263880315	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3902	0.11960952486633589	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3328	0.10201447943494768	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.065338925328957E-5
2	0.0	0.0	0.0	0.0	3.065338925328957E-5
3	0.0	0.0	0.0	0.0	3.065338925328957E-5
4	0.0	0.0	0.0	0.0	3.065338925328957E-5
5	0.0	0.0	0.0	0.0	3.065338925328957E-5
6	0.0	0.0	0.0	0.0	3.065338925328957E-5
7	0.0	0.0	0.0	0.0	3.065338925328957E-5
8	0.0	0.0	0.0	0.0	3.065338925328957E-5
9	0.0	0.0	0.0	3.065338925328957E-5	3.065338925328957E-5
10	0.0	0.0	0.0	6.130677850657913E-5	3.065338925328957E-5
11	0.0	0.0	0.0	6.130677850657913E-5	3.065338925328957E-5
12	0.0	0.0	0.0	9.196016775986871E-5	6.130677850657913E-5
13	0.0	0.0	0.0	9.196016775986871E-5	6.130677850657913E-5
14	0.0	0.0	0.0	9.196016775986871E-5	1.2261355701315827E-4
15	0.0	0.0	0.0	1.5326694626644784E-4	1.2261355701315827E-4
16	0.0	0.0	0.0	7.050279528256601E-4	1.2261355701315827E-4
17	0.0	0.0	0.0	0.0013180957378914515	1.2261355701315827E-4
18	0.0	0.0	0.0	0.0016552830196776367	1.2261355701315827E-4
19	0.0	3.065338925328957E-5	0.0	0.002053777079970401	1.2261355701315827E-4
20	0.0	3.065338925328957E-5	0.0	0.0026055380865296135	1.2261355701315827E-4
21	0.0	3.065338925328957E-5	0.0	0.004046247381434223	1.5326694626644784E-4
22	0.0	3.065338925328957E-5	0.0	0.006498518521697388	1.5326694626644784E-4
23	0.0	3.065338925328957E-5	0.0	0.010422152346118454	1.8392033551973741E-4
24	0.0	3.065338925328957E-5	0.0	0.014805587009338862	1.8392033551973741E-4
25	0.0	3.065338925328957E-5	0.0	0.016706097143042813	1.8392033551973741E-4
26	0.0	3.065338925328957E-5	0.0	0.02124279875252967	1.8392033551973741E-4
27	0.0	3.065338925328957E-5	0.0	0.03773432217079946	1.8392033551973741E-4
28	0.0	3.065338925328957E-5	0.0	0.0672841894109706	1.8392033551973741E-4
29	0.0	3.065338925328957E-5	0.0	0.10722555560800691	1.8392033551973741E-4
30	0.0	3.065338925328957E-5	0.0	0.18226505250005978	1.8392033551973741E-4
31	0.0	3.065338925328957E-5	0.0	0.4173458946835375	1.8392033551973741E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAGACG	700	0.0	18.764284	4
CTTATAC	1630	0.0	18.5	37
AAGACGG	670	0.0	17.119404	5
ACGGACC	730	0.0	15.965754	8
CGACTAT	70	0.0025938633	15.857143	36
TCGTTAC	935	0.0	15.037433	23
GGGCGTC	960	0.0	14.645833	11
TCTTATA	2665	0.0	14.439025	37
TACGGAT	1320	0.0	14.435606	27
ACGGATG	1375	0.0	14.127273	28
GGCGTCA	1000	0.0	14.060001	12
CGTTACG	740	0.0	14.0	24
AGACGGA	875	0.0	13.954286	6
GAACAGT	2095	0.0	13.952267	1
TACTTAC	1070	0.0	13.831776	31
TAGTACT	175	3.5724952E-8	13.742857	4
AGGGCGT	1045	0.0	13.631578	10
AACAGTG	2145	0.0	13.627039	2
GACGGAC	815	0.0	13.619632	7
ACAGTGG	2315	0.0	13.5054	3
>>END_MODULE