FastQCFastQC Report
Thu 9 Feb 2017
SRR2727271.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727271.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5990731
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT380420.6350143246291646No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT246770.4119196805865595No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT239390.39960064973706877No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118140.19720464831420406No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT72860.12162121784470042No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG18250.016.5232895
CTTATAC27850.016.40754137
CAAGACG19800.015.8838394
ACGGACC19750.014.9873418
CGCATCG13750.014.53090913
TCTTATA44100.014.38888937
TCGTTTA13100.014.26335930
CGCAAGA21800.014.1720182
CTAGCGG10650.014.07042229
TCTAGCG10700.014.00467328
TAACGCC14900.013.906044
ATAACGC16350.013.8042813
ATACCGT14550.013.7319586
TACCGTC13800.013.6739147
GACGGAC21650.013.5866057
GCGCAAG23500.013.5404241
CGGACCA21550.013.4779599
TCGTTAC11000.013.45454623
TTAGAGT10400.013.3413464
TACGGAT18050.013.324127
Produced by FastQC (version 0.11.5)