##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727271.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5990731
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.202448248803027	31.0	31.0	34.0	30.0	34.0
2	31.437072704482976	31.0	31.0	34.0	30.0	34.0
3	31.586742920020946	31.0	31.0	34.0	30.0	34.0
4	35.40417521668057	37.0	35.0	37.0	33.0	37.0
5	35.22203834557085	37.0	35.0	37.0	33.0	37.0
6	35.22561904381953	37.0	35.0	37.0	32.0	37.0
7	35.12730883760263	37.0	35.0	37.0	32.0	37.0
8	35.13249952301314	37.0	35.0	37.0	32.0	37.0
9	36.698518094035606	39.0	35.0	39.0	32.0	39.0
10	36.432707293984656	38.0	35.0	39.0	32.0	39.0
11	36.58702535633798	39.0	35.0	39.0	32.0	39.0
12	36.43774991733062	38.0	35.0	39.0	32.0	39.0
13	36.50141593738727	38.0	35.0	39.0	32.0	39.0
14	37.51673243215227	39.0	36.0	41.0	32.0	41.0
15	37.52213978561214	39.0	36.0	41.0	32.0	41.0
16	37.47014646459672	39.0	36.0	41.0	32.0	41.0
17	37.45140684834622	39.0	36.0	41.0	32.0	41.0
18	37.385350135066986	39.0	36.0	41.0	32.0	41.0
19	37.402842491175115	39.0	36.0	41.0	32.0	41.0
20	37.32075768382857	39.0	36.0	41.0	31.0	41.0
21	37.21730386492066	39.0	36.0	40.0	31.0	41.0
22	37.14373871903112	39.0	36.0	40.0	31.0	41.0
23	37.03850915689588	39.0	36.0	40.0	31.0	41.0
24	36.90190245564356	39.0	36.0	40.0	30.0	41.0
25	36.78220788080787	39.0	35.0	40.0	30.0	41.0
26	36.558299980419754	38.0	35.0	40.0	30.0	41.0
27	36.39569211169722	38.0	35.0	40.0	30.0	41.0
28	36.183513831617546	38.0	35.0	40.0	29.0	41.0
29	36.12501162212091	38.0	35.0	40.0	29.0	41.0
30	36.03629740677724	38.0	35.0	40.0	29.0	41.0
31	36.16937031557585	38.0	35.0	40.0	30.0	41.0
32	36.15023141583222	38.0	35.0	40.0	30.0	41.0
33	36.14142297492577	38.0	35.0	40.0	30.0	41.0
34	36.188941049097345	38.0	35.0	40.0	30.0	41.0
35	35.97963887879459	38.0	35.0	40.0	29.0	41.0
36	35.94452062694853	38.0	35.0	40.0	28.0	41.0
37	35.86131508825885	38.0	34.0	40.0	28.0	41.0
38	35.765098282663665	38.0	34.0	40.0	27.0	41.0
39	35.69236392019605	38.0	34.0	40.0	27.0	41.0
40	35.49900070625772	38.0	34.0	40.0	27.0	41.0
41	35.431691725099995	38.0	34.0	40.0	26.0	41.0
42	35.22045957329748	38.0	33.0	40.0	26.0	41.0
43	34.86584057938839	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	5.0
14	7.0
15	45.0
16	127.0
17	247.0
18	444.0
19	968.0
20	1978.0
21	4005.0
22	7591.0
23	13122.0
24	21749.0
25	34200.0
26	51361.0
27	73563.0
28	102304.0
29	136340.0
30	175941.0
31	220901.0
32	271530.0
33	332115.0
34	406431.0
35	496468.0
36	615242.0
37	790508.0
38	1071523.0
39	1162013.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.512608227610286	19.319729094829995	12.496488325047478	25.67117435251224
2	18.42649920351957	22.638088740756345	33.54991235627172	25.385499699452367
3	20.1487765015655	24.15825380909275	30.229916849880254	25.463052839461497
4	14.631135999930558	15.653315096271223	34.33691147207244	35.37863743172578
5	14.317251100074433	37.95057731685832	33.24021726230071	14.491954320766531
6	34.53157886742035	35.201447035428565	15.633150612170702	14.633823484980379
7	28.58305605776657	31.84704838190865	20.945340393350996	18.62455516697378
8	28.531826917282714	32.88465130549177	19.81192278538295	18.771598991842563
9	28.178864983254964	13.070057727512719	19.01674436725668	39.734332921975636
10	16.930755194983718	26.963771199207574	32.34202971223378	23.763443893574923
11	36.87798701026636	21.911833464063065	20.570043956238397	20.64013556943218
12	21.86212333686824	25.670005880751447	28.96401123669215	23.50385954568816
13	30.637980573656204	19.189778342576222	24.739685357262744	25.43255572650483
14	23.035803143222424	20.72932334968804	25.00345951103463	31.231413996054908
15	25.8532222528436	27.56182843128827	22.12818435680053	24.456764959067602
16	24.708069849906465	26.010398397123822	24.58361091492841	24.697920838041302
17	24.114920199221096	26.02470382996666	25.234900381940033	24.62547558887221
18	24.02536518498327	24.66558755517482	26.936562499634853	24.372484760207062
19	24.501567504867104	25.36456736248047	27.00251438430469	23.13135074834774
20	24.231249909234784	24.49615914986001	26.535693223414636	24.73689771749057
21	24.956169789630014	25.33230084942889	26.540433880272708	23.171095480668384
22	25.268902910179076	24.741504834718835	25.704959878852847	24.28463237624924
23	23.76918609765653	24.963547854176728	26.49205914937593	24.775206898790817
24	23.73211215793198	25.65790385180039	26.33207867286981	24.277905317397828
25	24.445447475441647	24.692111864144792	25.826414172160295	25.03602648825327
26	23.87852166955919	25.803695742639754	26.442098635375217	23.875683952425838
27	24.931565112838484	25.405914570358778	25.628792212502947	24.033728104299794
28	23.553870137050055	26.11357445360174	26.313767051132825	24.018788358215385
29	23.76608130126357	26.07528196475522	26.545959082455884	23.61267765152533
30	23.949898601689846	26.315352834236755	26.28098307201575	23.453765492057645
31	24.14606831787306	26.03817464012322	25.809955412786856	24.00580162921687
32	23.50330869471522	26.115110159344496	25.694577172635526	24.687003973304762
33	23.113623362491158	25.64501727752423	26.40831310903461	24.83304625095001
34	24.10118230980493	25.849716837561225	26.60995795003982	23.439142902594025
35	24.133765979477296	25.718330534287055	26.72814052241705	23.419762963818606
36	23.139363126136026	26.311229798166536	26.488587119001007	24.060819956696434
37	23.499920126608924	25.986227724129158	26.795110646764144	23.718741502497775
38	23.34471369186832	25.366637226742444	26.900606954309918	24.388042127079316
39	23.306888591726118	25.245867324037754	27.071854837080817	24.37538924715531
40	23.650035362963216	25.21910932071562	27.450105838502846	23.680749477818317
41	22.61842503026759	25.466808641549754	27.827038136080557	24.087728192102098
42	23.235762046401348	25.468361039746235	27.7172518679273	23.578625045925115
43	22.52422951389405	24.550125852754864	27.75228932829733	25.173355305053757
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	877.0
1	968.5
2	1060.0
3	2153.5
4	3247.0
5	3247.0
6	5250.0
7	7253.0
8	7084.0
9	6915.0
10	9452.0
11	11989.0
12	11989.0
13	22314.5
14	32640.0
15	54008.0
16	75376.0
17	66646.0
18	57916.0
19	57916.0
20	61118.0
21	64320.0
22	54544.5
23	44769.0
24	53236.0
25	61703.0
26	61703.0
27	71123.5
28	80544.0
29	90157.5
30	99771.0
31	111586.0
32	123401.0
33	123401.0
34	142125.5
35	160850.0
36	188280.5
37	215711.0
38	244786.0
39	273861.0
40	273861.0
41	301656.5
42	329452.0
43	348956.0
44	368460.0
45	402312.5
46	436165.0
47	436165.0
48	478880.5
49	521596.0
50	528826.0
51	536056.0
52	517085.0
53	498114.0
54	498114.0
55	477964.5
56	457815.0
57	435542.5
58	413270.0
59	383090.0
60	352910.0
61	352910.0
62	298816.0
63	244722.0
64	215158.5
65	185595.0
66	155299.5
67	125004.0
68	125004.0
69	102642.0
70	80280.0
71	66929.5
72	53579.0
73	39748.5
74	25918.0
75	25918.0
76	20257.5
77	14597.0
78	12633.5
79	10670.0
80	8290.0
81	5910.0
82	5910.0
83	4870.0
84	3830.0
85	3335.5
86	2841.0
87	1919.5
88	998.0
89	998.0
90	686.5
91	375.0
92	278.5
93	182.0
94	178.0
95	174.0
96	174.0
97	91.5
98	9.0
99	7.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5990731.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.65366465946291
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.70647702446082	37.185992502863805
2	10.375259911989342	9.68087793377439
3	3.4955971812300057	4.892472560330054
4	1.6998083724102737	3.1720915916710544
5	1.0090534396038424	2.353802039737764
6	0.6731291800271365	1.8842365822491163
7	0.48087465086766434	1.5704195293371421
8	0.35335928804470135	1.3188404582995246
9	0.29568507917104514	1.2415313275607414
>10	1.6950303247193395	14.695774704930741
>50	0.12394560243480925	3.9512832113540073
>100	0.07447775394615701	6.829985854603065
>500	0.009707865064546973	3.2420636135741336
>1k	0.007415214567423383	6.21315679384934
>5k	3.5822292595168956E-5	0.12176663573132716
>10k+	1.4328917038067583E-4	1.6457046601338523
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	38042	0.6350143246291646	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	24677	0.4119196805865595	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23939	0.39960064973706877	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11814	0.19720464831420406	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	7286	0.12162121784470042	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6692453725597094E-5
2	0.0	0.0	0.0	0.0	1.6692453725597094E-5
3	0.0	0.0	0.0	0.0	1.6692453725597094E-5
4	0.0	0.0	0.0	0.0	3.338490745119419E-5
5	0.0	0.0	0.0	0.0	3.338490745119419E-5
6	0.0	0.0	0.0	0.0	1.0015472235358257E-4
7	0.0	1.6692453725597094E-5	0.0	1.6692453725597094E-5	1.0015472235358257E-4
8	0.0	1.6692453725597094E-5	0.0	1.6692453725597094E-5	1.0015472235358257E-4
9	0.0	1.6692453725597094E-5	0.0	1.6692453725597094E-5	1.6692453725597093E-4
10	0.0	1.6692453725597094E-5	0.0	5.007736117679128E-5	1.6692453725597093E-4
11	0.0	1.6692453725597094E-5	0.0	5.007736117679128E-5	1.6692453725597093E-4
12	0.0	1.6692453725597094E-5	0.0	5.007736117679128E-5	1.8361699098156802E-4
13	0.0	1.6692453725597094E-5	0.0	6.676981490238838E-5	2.0030944470716513E-4
14	0.0	1.6692453725597094E-5	0.0	8.346226862798546E-5	3.004641670607477E-4
15	0.0	1.6692453725597094E-5	0.0	1.8361699098156802E-4	4.1731134313992735E-4
16	0.0	1.6692453725597094E-5	0.0	5.50850972944704E-4	4.506962505911215E-4
17	0.0	1.6692453725597094E-5	0.0	9.514698623590344E-4	4.506962505911215E-4
18	0.0	1.6692453725597094E-5	0.0	0.0011350868533406024	4.840811580423157E-4
19	0.0	1.6692453725597094E-5	0.0	0.0013353962980477674	4.840811580423157E-4
20	0.0	1.6692453725597094E-5	0.0	0.0016692453725597094	4.840811580423157E-4
21	0.0	1.6692453725597094E-5	0.0	0.002537252966290758	4.840811580423157E-4
22	0.0	1.6692453725597094E-5	0.0	0.003989496440417705	5.007736117679128E-4
23	0.0	1.6692453725597094E-5	0.0	0.006426594684354881	5.842358803958982E-4
24	0.0	1.6692453725597094E-5	0.0	0.00913077218790161	5.842358803958982E-4
25	0.0	1.6692453725597094E-5	0.0	0.010416091124772587	5.842358803958982E-4
26	0.0	1.6692453725597094E-5	0.0	0.013487502610282451	5.842358803958982E-4
27	0.0	1.6692453725597094E-5	0.0	0.026691233507229753	5.842358803958982E-4
28	0.0	1.6692453725597094E-5	0.0	0.04944304793521859	5.842358803958982E-4
29	0.0	1.6692453725597094E-5	0.0	0.08252749121935203	5.842358803958982E-4
30	0.0	1.6692453725597094E-5	0.0	0.13762928096754803	5.842358803958982E-4
31	0.0	1.6692453725597094E-5	0.0	0.318391862362039	5.842358803958982E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	1825	0.0	16.523289	5
CTTATAC	2785	0.0	16.407541	37
CAAGACG	1980	0.0	15.883839	4
ACGGACC	1975	0.0	14.987341	8
CGCATCG	1375	0.0	14.530909	13
TCTTATA	4410	0.0	14.388889	37
TCGTTTA	1310	0.0	14.263359	30
CGCAAGA	2180	0.0	14.172018	2
CTAGCGG	1065	0.0	14.070422	29
TCTAGCG	1070	0.0	14.004673	28
TAACGCC	1490	0.0	13.90604	4
ATAACGC	1635	0.0	13.804281	3
ATACCGT	1455	0.0	13.731958	6
TACCGTC	1380	0.0	13.673914	7
GACGGAC	2165	0.0	13.586605	7
GCGCAAG	2350	0.0	13.540424	1
CGGACCA	2155	0.0	13.477959	9
TCGTTAC	1100	0.0	13.454546	23
TTAGAGT	1040	0.0	13.341346	4
TACGGAT	1805	0.0	13.3241	27
>>END_MODULE