FastQCFastQC Report
Thu 9 Feb 2017
SRR2727270.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727270.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3302029
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT264890.802203735945384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT167930.5085660967847344No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT144260.4368828983634002No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89050.26968267086691244No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT39240.11883602475932223No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATG38470.11650412519090535No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA37880.1147173450021184No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA37280.112900280403352No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG36590.11081065611477064No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC35580.10775193070684722No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA33970.1028761407001574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG8150.018.840495
CAAGACG9800.016.9897964
ATCGATA904.4500157E-516.44444523
CGAACGA10600.016.05660416
CGAACGT3800.015.5789484
ACGAACG11150.015.26457415
TAACGAA11200.015.19642813
CTTATAC16350.015.16208137
GTTACGG8000.015.03125125
TACGGAT9750.014.827
AACGAAC11500.014.814
GTACTAG1252.9626262E-614.7999991
CGCTTCG5850.014.54700932
ATAACGA11650.014.45064412
ACGGATG10250.014.43902428
TATACTG3900.014.230775
TCTTATA25400.014.20275537
CGTTACG5750.014.15652224
CCGATAA11900.014.14705859
TCGTTAC6500.013.94615423
Produced by FastQC (version 0.11.5)