##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727270.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3302029
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.926268364087658	31.0	31.0	33.0	28.0	34.0
2	31.160558856387997	31.0	31.0	34.0	28.0	34.0
3	31.294217585611754	31.0	31.0	34.0	28.0	34.0
4	35.14595631958411	35.0	35.0	37.0	32.0	37.0
5	34.950918056746325	35.0	35.0	37.0	32.0	37.0
6	34.977922967969086	36.0	35.0	37.0	32.0	37.0
7	34.889127260844774	35.0	35.0	37.0	32.0	37.0
8	34.90571978622841	36.0	35.0	37.0	32.0	37.0
9	36.41248426346346	38.0	35.0	39.0	32.0	39.0
10	36.12672844484406	38.0	35.0	39.0	31.0	39.0
11	36.30178959663891	38.0	35.0	39.0	32.0	39.0
12	36.14017442003084	38.0	35.0	39.0	31.0	39.0
13	36.21698810034679	38.0	35.0	39.0	32.0	39.0
14	37.13099672958656	39.0	36.0	40.0	31.0	41.0
15	37.142416374901615	39.0	36.0	40.0	31.0	41.0
16	37.07589061149978	39.0	36.0	40.0	31.0	41.0
17	37.070063588175636	39.0	36.0	40.0	31.0	41.0
18	37.00590212866089	39.0	36.0	40.0	31.0	41.0
19	37.02374691439718	39.0	36.0	40.0	31.0	41.0
20	36.942353322760034	39.0	36.0	40.0	31.0	41.0
21	36.82787916157005	39.0	36.0	40.0	30.0	41.0
22	36.74186447181415	39.0	35.0	40.0	30.0	41.0
23	36.610049457469934	38.0	35.0	40.0	30.0	41.0
24	36.45298663336997	38.0	35.0	40.0	30.0	41.0
25	36.31996296822348	38.0	35.0	40.0	30.0	41.0
26	36.06140194407742	38.0	34.0	40.0	29.0	41.0
27	35.87714129706311	38.0	34.0	40.0	28.0	41.0
28	35.620564810303	38.0	34.0	40.0	27.0	41.0
29	35.54595704640995	38.0	34.0	40.0	27.0	41.0
30	35.44068450034812	38.0	34.0	40.0	27.0	41.0
31	35.572728161987676	38.0	34.0	40.0	27.0	41.0
32	35.538544634223385	38.0	34.0	40.0	27.0	41.0
33	35.477682055487705	38.0	34.0	40.0	27.0	41.0
34	35.52265531283947	38.0	34.0	40.0	27.0	41.0
35	35.263818700562595	38.0	34.0	40.0	26.0	41.0
36	35.214091396532254	38.0	33.0	40.0	26.0	41.0
37	35.11645900142003	38.0	33.0	40.0	25.0	41.0
38	34.99638616135715	38.0	33.0	40.0	25.0	41.0
39	34.884095808970784	38.0	33.0	40.0	25.0	41.0
40	34.64903185283957	38.0	33.0	40.0	24.0	41.0
41	34.55804991415884	37.0	33.0	40.0	24.0	41.0
42	34.28776428068924	37.0	33.0	40.0	23.0	41.0
43	33.941621045726734	37.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	4.0
14	8.0
15	26.0
16	89.0
17	191.0
18	376.0
19	944.0
20	1743.0
21	3486.0
22	6292.0
23	10516.0
24	17077.0
25	25845.0
26	37313.0
27	52074.0
28	69602.0
29	91108.0
30	114120.0
31	140441.0
32	168120.0
33	201603.0
34	242002.0
35	289056.0
36	353100.0
37	443422.0
38	577610.0
39	455859.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.285797308261074	20.839217341822255	13.61826319514456	24.256722154772113
2	19.288080147085324	23.928045453265252	32.54595886347455	24.237915536174878
3	21.194362617651148	24.962530613752936	30.274809821476435	23.56829694711948
4	15.39838081373604	16.67810912623723	33.807728520857935	34.11578153916879
5	14.092244495732775	39.12367214218894	32.640597644660296	14.143485717417986
6	33.77583903714959	35.43539441961291	15.84774088901097	14.94102565422654
7	27.969893662351243	32.698471152131006	20.791579964924598	18.540055220593157
8	28.891508826845552	32.66603654904303	19.80621611742356	18.636238506687857
9	28.395571329022246	13.14737090437425	19.622086904748564	38.83497086185494
10	17.467896254091045	27.588824931579946	31.635124948932912	23.308153865396093
11	36.60061737798184	22.520335224190944	20.210906687978817	20.668140709848398
12	21.44393644029171	26.66242483030888	28.605866271919478	23.28777245747993
13	30.625291298168488	19.453523878803004	25.155624011781846	24.76556081124666
14	23.05748980399627	21.433276327978948	25.309680805347256	30.199553062677527
15	26.13614235368617	28.161200280191363	22.196292037410934	23.506365328711528
16	24.53370336844407	26.223452307656895	25.025461617690215	24.217382706208817
17	23.953847770567734	26.648160873208564	25.166102417634733	24.231888938588973
18	23.48241036041779	24.63842685815297	27.24748934670168	24.631673434727556
19	23.91120732131668	25.895532716399522	27.467596438432253	22.725663523851548
20	23.898669575585192	24.53318853347442	26.928715647258095	24.639426243682294
21	24.925644202397983	25.581543953732687	27.175806148280344	22.317005695588986
22	25.294054049797865	25.095146045052907	26.01415675028899	23.59664315486024
23	23.673050721238365	25.38463472004637	26.782108818547627	24.160205740167637
24	23.059428006234956	26.211126552795267	26.86012145865466	23.869323982315116
25	24.037705301800802	25.231698449650196	26.174997251689796	24.555598996859203
26	23.30633680079733	26.5411660527512	26.783441332586722	23.369055813864748
27	24.7937858813475	25.9173677759947	25.969941511719007	23.318904830938795
28	23.26502886558537	26.62575040982378	26.688560276121137	23.420660448469715
29	23.210062661472687	26.5270232332908	27.080622247714963	23.18229185752154
30	23.43719573631849	27.064904638935637	26.697827305574844	22.80007231917103
31	23.76132977632843	26.791254710361418	26.10379860382813	23.34361690948202
32	23.279535097965525	26.83489454514179	25.76367439534904	24.121895961543643
33	22.616427657055706	26.258339947953214	26.728535697294	24.39669669769708
34	23.81893072410933	26.500645512198712	27.01160407737182	22.668819686320138
35	23.48734671924444	26.21624461808179	27.43498012888439	22.861428533789375
36	22.419881836289143	27.015329059799292	26.874385415754983	23.690403688156586
37	23.205519999975774	26.674902007220407	27.111088364154284	23.008489628649535
38	22.904856377699893	25.877271217181917	27.159846264221184	24.058026140897006
39	22.99537647912844	26.069304660861548	27.21669010175259	23.718628758257424
40	23.015000776795116	25.758495761242557	27.78585530290618	23.440648159056142
41	21.963404924669046	26.492014455354575	28.174495136172332	23.370085483804047
42	22.638747267210555	25.966367951341436	28.162684216280354	23.23220056516766
43	21.86855415261344	25.04366254808786	28.05705219427207	25.03073110502664
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1677.0
1	1485.0
2	1293.0
3	1780.5
4	2268.0
5	2268.0
6	3706.5
7	5145.0
8	5164.5
9	5184.0
10	7206.0
11	9228.0
12	9228.0
13	17413.5
14	25599.0
15	40685.5
16	55772.0
17	47995.5
18	40219.0
19	40219.0
20	43465.5
21	46712.0
22	39348.5
23	31985.0
24	36924.0
25	41863.0
26	41863.0
27	46968.0
28	52073.0
29	56847.0
30	61621.0
31	66878.5
32	72136.0
33	72136.0
34	80482.0
35	88828.0
36	101154.0
37	113480.0
38	125875.5
39	138271.0
40	138271.0
41	157498.0
42	176725.0
43	188779.0
44	200833.0
45	219725.5
46	238618.0
47	238618.0
48	261876.5
49	285135.0
50	284041.5
51	282948.0
52	278911.5
53	274875.0
54	274875.0
55	258440.0
56	242005.0
57	231001.0
58	219997.0
59	200895.5
60	181794.0
61	181794.0
62	155917.5
63	130041.0
64	117384.0
65	104727.0
66	87750.5
67	70774.0
68	70774.0
69	56353.0
70	41932.0
71	34729.0
72	27526.0
73	19713.0
74	11900.0
75	11900.0
76	9172.0
77	6444.0
78	5662.0
79	4880.0
80	3871.5
81	2863.0
82	2863.0
83	2419.5
84	1976.0
85	1793.5
86	1611.0
87	1104.5
88	598.0
89	598.0
90	425.5
91	253.0
92	180.5
93	108.0
94	103.5
95	99.0
96	99.0
97	55.0
98	11.0
99	6.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3302029.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.68671992764771
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.1523897603998	37.887388922030915
2	9.49175354794652	8.862776790304713
3	3.2265101421255333	4.519055260473888
4	1.6123127919547156	3.010943830150141
5	0.9896431903822399	2.3101597228839688
6	0.6397363303092233	1.79203145284127
7	0.4738928633064703	1.5487152369430153
8	0.3510365596246092	1.3110996434847306
9	0.25926400180000203	1.089376725542206
>10	1.5819085098306571	13.602201732319209
>50	0.11257509376112068	3.6594577341855645
>100	0.0885216472332449	8.466973608375698
>500	0.013224455252300584	4.3469062967456225
>1k	0.00697052567461321	5.566937916115924
>5k	6.514509976274029E-5	0.27083765197943754
>10k+	1.9543529928822085E-4	1.7551374756237377
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	26489	0.802203735945384	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16793	0.5085660967847344	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	14426	0.4368828983634002	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8905	0.26968267086691244	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	3924	0.11883602475932223	No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATG	3847	0.11650412519090535	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	3788	0.1147173450021184	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	3728	0.112900280403352	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	3659	0.11081065611477064	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	3558	0.10775193070684722	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	3397	0.1028761407001574	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.0284409979439913E-5
2	0.0	0.0	0.0	0.0	3.0284409979439913E-5
3	0.0	0.0	0.0	0.0	3.0284409979439913E-5
4	0.0	0.0	0.0	0.0	3.0284409979439913E-5
5	0.0	0.0	0.0	0.0	3.0284409979439913E-5
6	0.0	0.0	0.0	0.0	3.0284409979439913E-5
7	0.0	0.0	0.0	0.0	6.056881995887983E-5
8	0.0	0.0	0.0	0.0	9.085322993831975E-5
9	0.0	0.0	0.0	0.0	9.085322993831975E-5
10	0.0	0.0	0.0	0.0	9.085322993831975E-5
11	0.0	0.0	0.0	0.0	9.085322993831975E-5
12	0.0	0.0	0.0	0.0	9.085322993831975E-5
13	0.0	0.0	0.0	0.0	9.085322993831975E-5
14	0.0	0.0	0.0	6.056881995887983E-5	1.5142204989719956E-4
15	0.0	0.0	0.0	2.119908698560794E-4	1.817064598766395E-4
16	0.0	0.0	0.0	4.845505596710386E-4	1.817064598766395E-4
17	0.0	0.0	0.0	7.873946594654377E-4	2.119908698560794E-4
18	0.0	0.0	0.0	9.993855293215172E-4	2.119908698560794E-4
19	0.0	0.0	0.0	0.0012719452191364763	2.119908698560794E-4
20	0.0	0.0	0.0	0.0016353581388897553	2.119908698560794E-4
21	0.0	0.0	0.0	0.002422752798355193	2.422752798355193E-4
22	0.0	0.0	0.0	0.003876404477368309	2.422752798355193E-4
23	0.0	0.0	0.0	0.006026597585908543	2.7255968981495924E-4
24	0.0	0.0	0.0	0.008933900943934774	2.7255968981495924E-4
25	3.0284409979439913E-5	0.0	0.0	0.010266414983030131	3.028440997943991E-4
26	3.0284409979439913E-5	0.0	0.0	0.013658268900727402	3.028440997943991E-4
27	3.0284409979439913E-5	0.0	0.0	0.026710849601866003	3.028440997943991E-4
28	3.0284409979439913E-5	0.0	0.0	0.051786341064842256	3.028440997943991E-4
29	3.0284409979439913E-5	0.0	0.0	0.0882487706800879	3.028440997943991E-4
30	3.0284409979439913E-5	0.0	0.0	0.15481390381489685	3.028440997943991E-4
31	3.0284409979439913E-5	0.0	0.0	0.36023305670543776	3.028440997943991E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	815	0.0	18.84049	5
CAAGACG	980	0.0	16.989796	4
ATCGATA	90	4.4500157E-5	16.444445	23
CGAACGA	1060	0.0	16.056604	16
CGAACGT	380	0.0	15.578948	4
ACGAACG	1115	0.0	15.264574	15
TAACGAA	1120	0.0	15.196428	13
CTTATAC	1635	0.0	15.162081	37
GTTACGG	800	0.0	15.031251	25
TACGGAT	975	0.0	14.8	27
AACGAAC	1150	0.0	14.8	14
GTACTAG	125	2.9626262E-6	14.799999	1
CGCTTCG	585	0.0	14.547009	32
ATAACGA	1165	0.0	14.450644	12
ACGGATG	1025	0.0	14.439024	28
TATACTG	390	0.0	14.23077	5
TCTTATA	2540	0.0	14.202755	37
CGTTACG	575	0.0	14.156522	24
CCGATAA	1190	0.0	14.1470585	9
TCGTTAC	650	0.0	13.946154	23
>>END_MODULE