FastQCFastQC Report
Thu 9 Feb 2017
SRR2727269.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727269.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3327293
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT256640.7713177048128915No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT165730.4980925935888424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT157280.47269657346076827No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79760.2397143864396673No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT41650.1251768329389687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG10150.020.7783265
CAAGACG10950.019.4292244
TAGTACT2650.018.1509444
ACGGACC11900.017.4117668
GACGGAC12300.017.1463417
GCGCAAG12500.016.8721
CGCAAGA12850.016.5564212
CGGACCA12750.016.1058839
AGAGCGA13100.015.81679415
AGACGGA13450.015.6802976
TATTCCG957.0661066E-515.5789485
CAGAGCG13400.015.46268714
CGAACGT3850.015.3766254
AGCGAAA13350.015.10486917
TATACCG1252.9626408E-614.8000015
GAGCGAA13750.014.79999916
CGAAAGC13900.014.77338119
GTAGTAC2151.2732926E-1114.6279073
GCAAGAC15950.014.4984323
CTTATAC15600.014.46794937
Produced by FastQC (version 0.11.5)