##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727269.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3327293
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2195201925409	31.0	31.0	34.0	30.0	34.0
2	31.4605155001378	31.0	31.0	34.0	30.0	34.0
3	31.61113012890659	31.0	31.0	34.0	30.0	34.0
4	35.42413517535125	37.0	35.0	37.0	33.0	37.0
5	35.24167754387726	37.0	35.0	37.0	33.0	37.0
6	35.2447494104066	37.0	35.0	37.0	32.0	37.0
7	35.14173924568711	37.0	35.0	37.0	32.0	37.0
8	35.14975356844137	37.0	35.0	37.0	32.0	37.0
9	36.72032850728806	39.0	35.0	39.0	32.0	39.0
10	36.447852052704704	38.0	35.0	39.0	32.0	39.0
11	36.61321590854788	39.0	35.0	39.0	32.0	39.0
12	36.46230494278682	38.0	35.0	39.0	32.0	39.0
13	36.52292118548021	38.0	35.0	39.0	32.0	39.0
14	37.531869600903796	39.0	36.0	41.0	32.0	41.0
15	37.52966901321886	39.0	36.0	41.0	32.0	41.0
16	37.48068985809185	39.0	36.0	41.0	32.0	41.0
17	37.46143696993322	39.0	36.0	41.0	32.0	41.0
18	37.40055624797696	39.0	36.0	41.0	32.0	41.0
19	37.41333179855216	39.0	36.0	41.0	32.0	41.0
20	37.334777850943695	39.0	36.0	41.0	31.0	41.0
21	37.23299961860888	39.0	36.0	41.0	31.0	41.0
22	37.16182223807762	39.0	36.0	40.0	31.0	41.0
23	37.049765379844814	39.0	36.0	40.0	31.0	41.0
24	36.906629503322975	39.0	36.0	40.0	31.0	41.0
25	36.774394981145335	39.0	35.0	40.0	30.0	41.0
26	36.54137222060095	38.0	35.0	40.0	30.0	41.0
27	36.37152423907362	38.0	35.0	40.0	30.0	41.0
28	36.140391002535694	38.0	35.0	40.0	29.0	41.0
29	36.06650841990771	38.0	35.0	40.0	29.0	41.0
30	35.96728902444119	38.0	34.0	40.0	29.0	41.0
31	36.096448374098706	38.0	35.0	40.0	30.0	41.0
32	36.066010116932894	38.0	35.0	40.0	29.0	41.0
33	36.04116168909681	38.0	35.0	40.0	29.0	41.0
34	36.07272127822828	38.0	35.0	40.0	29.0	41.0
35	35.85524148309151	38.0	34.0	40.0	28.0	41.0
36	35.81050661904437	38.0	34.0	40.0	28.0	41.0
37	35.72073574524396	38.0	34.0	40.0	27.0	41.0
38	35.61011909681534	38.0	34.0	40.0	27.0	41.0
39	35.52341347756269	38.0	34.0	40.0	27.0	41.0
40	35.31549731268031	38.0	34.0	40.0	26.0	41.0
41	35.22571682145215	38.0	34.0	40.0	26.0	41.0
42	34.99797462982671	38.0	33.0	40.0	25.0	41.0
43	34.62228995162133	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	9.0
15	13.0
16	71.0
17	122.0
18	239.0
19	567.0
20	1162.0
21	2363.0
22	4409.0
23	7656.0
24	12517.0
25	19171.0
26	28904.0
27	41758.0
28	57872.0
29	77164.0
30	99398.0
31	123506.0
32	153265.0
33	185576.0
34	228810.0
35	277652.0
36	343493.0
37	441873.0
38	590079.0
39	629644.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.8080725081921	19.069916595863365	12.59236863119659	25.52964226474795
2	18.449291961964278	22.569638441820423	33.446438290826805	25.534631305388494
3	20.25538478276485	24.057214077630075	30.33577145144717	25.35162968815791
4	14.663662021949975	15.562951624639007	34.03162871439335	35.74175763901766
5	14.305983873376945	37.97711833613691	33.094320217666436	14.622577572819706
6	34.6466632184181	34.85638325209111	15.6060797771642	14.890873752326591
7	28.343641512785318	31.98101279328271	20.89115686535571	18.784188828576262
8	28.73218559351401	32.58531785448411	19.810458531905667	18.872038020096216
9	28.19048397601293	13.008532762218417	19.14087517991352	39.66010808185513
10	17.217239359443248	26.87289637552208	32.0060481598705	23.903816105164168
11	36.902310677178114	21.942522044196288	20.34137059766002	20.813796680965577
12	21.81854738972492	25.649589621352852	28.807712455741047	23.724150533181177
13	30.803388820882322	19.009296746634575	24.78988174470959	25.397432687773513
14	23.09246585738016	20.757324347450016	24.994402356510232	31.155807438659593
15	26.043002524875327	27.48312216567642	22.236665060756597	24.237210248691653
16	24.637235133785936	25.88025761482382	24.732627995190086	24.74987925620016
17	24.048708664971798	25.909861259588503	25.31962168645803	24.72180838898167
18	23.913253206134836	24.472055812337537	27.145881051052612	24.468809930475015
19	24.284335644621617	25.27357825114891	27.291645190249252	23.150440913980226
20	24.114678208381406	24.20348914267544	26.834186228865324	24.847646420077822
21	24.945744183034076	25.20385190002804	26.967748256615813	22.882655660322072
22	25.31273921473101	24.451498560541555	26.022325055232585	24.213437169494842
23	23.700708053062954	24.842627324975588	26.801426865623196	24.65523775633826
24	23.57144381333414	25.44515917293728	26.73103931634515	24.252357697383427
25	24.428777387503896	24.417506964370137	26.11320974738323	25.040505900742737
26	23.733407307381707	25.685354430763986	26.779998034438208	23.801240227416102
27	25.03957421243034	25.122704853464967	25.91977923194621	23.917941702158483
28	23.4615647013954	26.106086839962693	26.516089806338066	23.91625865230384
29	23.650366829732157	25.88025761482382	26.911877012334052	23.55749854310997
30	23.917731320926652	26.09355412943796	26.660471440296963	23.32824310933843
31	24.102776641552158	25.921312009492404	26.096259030990055	23.879652317965387
32	23.507998844706492	25.85846813009855	25.99608751017719	24.637445515017763
33	22.95767159670038	25.50851397817986	26.66873641726172	24.865078007858042
34	24.070107441695097	25.790454883294018	26.897841578724808	23.24159609628608
35	24.045793382187863	25.456609922841178	27.11116814780063	23.386428547170325
36	22.8623688986813	26.15071771557239	26.934928784450303	24.05198460129601
37	23.370229192319403	25.87568933664694	27.14885644275993	23.605225028273736
38	23.281538475872125	25.142210199101793	27.196612982385382	24.3796383426407
39	23.249079657246895	25.090997396381987	27.43572627959125	24.22419666677987
40	23.68324641082105	24.880646219013475	27.774710552993078	23.6613968171724
41	22.446324985506237	25.400528297327586	28.214527545364952	23.93861917180122
42	23.132798944968176	25.240698670060013	28.10155282387214	23.52494956109967
43	22.39706572279628	24.281841124301344	28.005889472312777	25.315203680589597
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	523.0
1	574.5
2	626.0
3	1353.0
4	2080.0
5	2080.0
6	3446.0
7	4812.0
8	4620.5
9	4429.0
10	6083.5
11	7738.0
12	7738.0
13	14537.5
14	21337.0
15	35311.0
16	49285.0
17	42959.0
18	36633.0
19	36633.0
20	37611.0
21	38589.0
22	31712.5
23	24836.0
24	29174.5
25	33513.0
26	33513.0
27	38409.5
28	43306.0
29	47621.0
30	51936.0
31	57973.5
32	64011.0
33	64011.0
34	73423.0
35	82835.0
36	97712.5
37	112590.0
38	128208.5
39	143827.0
40	143827.0
41	160656.0
42	177485.0
43	189188.0
44	200891.0
45	222822.5
46	244754.0
47	244754.0
48	270812.5
49	296871.0
50	299042.5
51	301214.0
52	290197.5
53	279181.0
54	279181.0
55	267144.0
56	255107.0
57	242757.5
58	230408.0
59	212636.5
60	194865.0
61	194865.0
62	166064.0
63	137263.0
64	121047.5
65	104832.0
66	87613.0
67	70394.0
68	70394.0
69	57747.0
70	45100.0
71	37668.5
72	30237.0
73	22381.5
74	14526.0
75	14526.0
76	11243.5
77	7961.0
78	6845.5
79	5730.0
80	4518.0
81	3306.0
82	3306.0
83	2651.5
84	1997.0
85	1707.0
86	1417.0
87	952.0
88	487.0
89	487.0
90	332.0
91	177.0
92	130.5
93	84.0
94	88.0
95	92.0
96	92.0
97	49.0
98	6.0
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3327293.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.38894878116417
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.30633864586974	40.1563459496485
2	9.594307047434677	9.477054787130273
3	3.191589773544315	4.728877915682025
4	1.6051007790136624	3.1709696065324993
5	0.9597195761556527	2.369977049551606
6	0.6242920883884508	1.8499877986741928
7	0.44451583268315553	1.5367918784963437
8	0.331424976912576	1.3094984967626986
9	0.2618350984166626	1.1638584238330465
>10	1.4904410216805764	13.254527963134095
>50	0.09918404792239363	3.3640466801568616
>100	0.07386304658177639	7.466852296049543
>500	0.012202151155908121	4.164893820251091
>1k	0.004941871217413632	3.999345475159571
>5k	6.101075577053867E-5	0.2403373856460557
>10k+	1.8303226731161598E-4	1.746634473291577
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	25664	0.7713177048128915	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	16573	0.4980925935888424	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15728	0.47269657346076827	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7976	0.2397143864396673	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	4165	0.1251768329389687	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.0108923380057E-5
2	0.0	0.0	0.0	0.0	6.0108923380057E-5
3	0.0	0.0	0.0	0.0	6.0108923380057E-5
4	0.0	0.0	0.0	0.0	9.01633850700855E-5
5	0.0	0.0	0.0	0.0	9.01633850700855E-5
6	0.0	0.0	0.0	0.0	1.20217846760114E-4
7	0.0	0.0	0.0	0.0	1.20217846760114E-4
8	0.0	0.0	0.0	0.0	1.20217846760114E-4
9	0.0	0.0	0.0	3.00544616900285E-5	1.502723084501425E-4
10	0.0	0.0	0.0	3.00544616900285E-5	1.502723084501425E-4
11	0.0	0.0	0.0	9.01633850700855E-5	1.502723084501425E-4
12	0.0	0.0	0.0	9.01633850700855E-5	1.80326770140171E-4
13	0.0	0.0	0.0	1.80326770140171E-4	1.80326770140171E-4
14	0.0	0.0	0.0	2.704901552102565E-4	3.305990785903135E-4
15	0.0	0.0	0.0	4.80871387040456E-4	4.508169253504275E-4
16	0.0	0.0	0.0	7.81416003940741E-4	4.508169253504275E-4
17	0.0	0.0	0.0	0.001442614161121368	4.80871387040456E-4
18	0.0	0.0	0.0	0.001502723084501425	4.80871387040456E-4
19	0.0	0.0	0.0	0.001683049854641596	4.80871387040456E-4
20	0.0	0.0	0.0	0.0022540846267521376	5.109258487304845E-4
21	0.0	0.0	0.0	0.0038169166346336196	5.710347721105415E-4
22	0.0	0.0	0.0	0.005950783414625643	5.710347721105415E-4
23	0.0	0.0	0.0	0.008866066198558408	5.710347721105415E-4
24	0.0	0.0	0.0	0.01280320067995214	5.710347721105415E-4
25	0.0	0.0	0.0	0.014907012998254136	5.710347721105415E-4
26	0.0	0.0	0.0	0.01902447424978804	6.0108923380057E-4
27	0.0	0.0	0.0	0.03372110601621198	6.0108923380057E-4
28	0.0	0.0	0.0	0.05953788860794646	6.0108923380057E-4
29	0.0	3.00544616900285E-5	0.0	0.09346937585598863	6.0108923380057E-4
30	0.0	3.00544616900285E-5	0.0	0.1570045078687089	6.0108923380057E-4
31	0.0	3.00544616900285E-5	0.0	0.35001426084207193	6.311436954905985E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	1015	0.0	20.778326	5
CAAGACG	1095	0.0	19.429224	4
TAGTACT	265	0.0	18.150944	4
ACGGACC	1190	0.0	17.411766	8
GACGGAC	1230	0.0	17.146341	7
GCGCAAG	1250	0.0	16.872	1
CGCAAGA	1285	0.0	16.556421	2
CGGACCA	1275	0.0	16.105883	9
AGAGCGA	1310	0.0	15.816794	15
AGACGGA	1345	0.0	15.680297	6
TATTCCG	95	7.0661066E-5	15.578948	5
CAGAGCG	1340	0.0	15.462687	14
CGAACGT	385	0.0	15.376625	4
AGCGAAA	1335	0.0	15.104869	17
TATACCG	125	2.9626408E-6	14.800001	5
GAGCGAA	1375	0.0	14.799999	16
CGAAAGC	1390	0.0	14.773381	19
GTAGTAC	215	1.2732926E-11	14.627907	3
GCAAGAC	1595	0.0	14.498432	3
CTTATAC	1560	0.0	14.467949	37
>>END_MODULE