FastQCFastQC Report
Thu 9 Feb 2017
SRR2727268.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727268.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6500214
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT285200.4387547856116737No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT184840.2843598687673975No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT176420.2714064490799841No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89390.13751854938929703No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT83250.12807270652935426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC25950.016.18304437
TCGTTAC9400.016.13829823
AAGACGG22400.016.1049125
CGTTACG6900.015.8188424
CAAGACG23700.015.22151854
TATACCG2900.014.6724155
TCTAGCG13800.014.4782628
CTAGCGG13700.014.313868529
TCTTATA40700.014.27272737
GTTACGG13450.013.75464625
TACGGAT17650.013.62606227
ACGGACC25450.013.593328
GACGGAC26000.013.59038457
TACTTAC10900.013.57798231
ATCTCGT13500.013.56666820
TCTATAC5500.013.4545463
TATACTG6350.013.4015755
AGACGGA27550.013.3629776
CGCAATA15100.013.35430336
TAGAGTG10600.013.2641525
Produced by FastQC (version 0.11.5)