##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727268.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6500214
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21758360570898	31.0	31.0	34.0	30.0	34.0
2	31.46336612917667	31.0	31.0	34.0	30.0	34.0
3	31.61688415181408	31.0	31.0	34.0	30.0	34.0
4	35.42842774099437	37.0	35.0	37.0	33.0	37.0
5	35.24165512089294	37.0	35.0	37.0	33.0	37.0
6	35.245837752418616	37.0	35.0	37.0	32.0	37.0
7	35.14740037789525	37.0	35.0	37.0	32.0	37.0
8	35.1498262980265	37.0	35.0	37.0	32.0	37.0
9	36.72879154440146	39.0	35.0	39.0	32.0	39.0
10	36.45919826639554	38.0	35.0	39.0	32.0	39.0
11	36.61335365266436	39.0	35.0	39.0	32.0	39.0
12	36.462719073556656	38.0	35.0	39.0	32.0	39.0
13	36.52496225508883	38.0	35.0	39.0	32.0	39.0
14	37.555478481170006	39.0	36.0	41.0	32.0	41.0
15	37.555741703273156	39.0	36.0	41.0	32.0	41.0
16	37.50210516146084	39.0	36.0	41.0	32.0	41.0
17	37.465810664079676	39.0	36.0	41.0	32.0	41.0
18	37.390328226116864	39.0	36.0	41.0	32.0	41.0
19	37.39584404451915	39.0	36.0	41.0	32.0	41.0
20	37.30977380129331	39.0	36.0	41.0	31.0	41.0
21	37.19896144957689	39.0	36.0	40.0	31.0	41.0
22	37.124252678450276	39.0	36.0	40.0	31.0	41.0
23	37.014557520721624	39.0	36.0	40.0	31.0	41.0
24	36.87477319977465	39.0	36.0	40.0	30.0	41.0
25	36.744318725506574	39.0	35.0	40.0	30.0	41.0
26	36.52315369924744	38.0	35.0	40.0	30.0	41.0
27	36.361845010025824	38.0	35.0	40.0	30.0	41.0
28	36.13333361024729	38.0	35.0	40.0	29.0	41.0
29	36.07360296137943	38.0	35.0	40.0	29.0	41.0
30	35.98469650383818	38.0	34.0	40.0	29.0	41.0
31	36.149058784833855	38.0	35.0	40.0	30.0	41.0
32	36.13576014574289	38.0	35.0	40.0	30.0	41.0
33	36.14226377777716	38.0	35.0	40.0	29.0	41.0
34	36.19283965112533	38.0	35.0	40.0	30.0	41.0
35	35.98494726481313	38.0	35.0	40.0	28.0	41.0
36	35.955343008707096	38.0	35.0	40.0	28.0	41.0
37	35.87983287934828	38.0	34.0	40.0	28.0	41.0
38	35.784587707420094	38.0	34.0	40.0	27.0	41.0
39	35.717427610844815	38.0	34.0	40.0	27.0	41.0
40	35.527548939158	38.0	34.0	40.0	27.0	41.0
41	35.46868226184554	38.0	34.0	40.0	26.0	41.0
42	35.25924531100053	38.0	33.0	40.0	26.0	41.0
43	34.895596360365985	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	4.0
14	8.0
15	29.0
16	119.0
17	278.0
18	477.0
19	1022.0
20	2193.0
21	4418.0
22	8343.0
23	14619.0
24	24047.0
25	37123.0
26	55157.0
27	79170.0
28	110178.0
29	146485.0
30	189056.0
31	237614.0
32	294872.0
33	359549.0
34	441950.0
35	539125.0
36	668245.0
37	855923.0
38	1178471.0
39	1251736.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.37669713643274	19.133539295783187	12.364116012180522	26.125647555603553
2	18.800011814995628	22.147086234391665	33.220475510498574	25.832426440114126
3	20.327930741972494	23.611268798227258	29.909799892741994	26.15100056705825
4	14.942046523391383	15.504843379002601	33.98091201305065	35.57219808455537
5	14.703669756103416	37.28783390823748	33.26664322128472	14.741853114374386
6	35.24614728068953	34.75307120657874	15.316418813288301	14.684362699443435
7	28.9893840418177	31.29109287786525	20.830560347705475	18.88896273261157
8	28.468308889522714	32.70752316769879	19.73411952283417	19.090048419944328
9	28.08859831384013	13.069600477768887	18.712768533466743	40.12903267492424
10	17.157942800037045	26.366393475660953	32.25918408224714	24.21647964205486
11	37.36893893031829	21.4669701643669	20.288501270881234	20.875589634433574
12	22.041705088478626	25.169848254226707	28.548137030565456	24.24030962672921
13	30.900536505413513	18.788519885652995	24.324306861281798	25.986636747651694
14	23.280633529911476	20.39983606693564	24.296876995126624	32.02265340802626
15	26.35374773815139	27.23165729620594	21.469447005898576	24.945147959744094
16	25.2580915028336	25.540343748682737	24.152081762231212	25.049482986252453
17	24.598097847240105	25.61426439191079	24.66163421696578	25.126003543883325
18	24.58339063913896	24.350321389418873	26.189568528051538	24.876719443390634
19	25.105019619354067	25.10771183841024	26.051865369355532	23.735403172880154
20	24.755938804476283	24.127128737607716	25.62434098323532	25.49259147468068
21	25.550728022185115	25.038298739087665	25.65304773042857	23.757925508298648
22	25.80453505069218	24.370197658107873	24.777045801876678	25.048221489323275
23	24.250016999440327	24.553868534174413	25.6726470851575	25.523467381227753
24	24.349767561498744	25.309828261038792	25.449977493048692	24.890426684413775
25	25.09900443277714	24.299630750618366	24.88484225288583	25.71652256371867
26	24.54397655215659	25.40960959131499	25.54706660426872	24.499347252259696
27	25.72555303563852	24.822444307218195	24.6502345922765	24.80176806486679
28	24.16486595672081	25.687769664198747	25.455577308685527	24.691787070394913
29	24.38796630387861	25.475084358761112	25.772243806126998	24.36470553123328
30	24.65644977226904	25.7935354128341	25.479853432517764	24.070161382379105
31	25.012868807088505	25.40900961106819	24.906533846424132	24.671587735419173
32	24.15658930613669	25.482253353504973	24.860735354251414	25.500421986106918
33	23.76881745739448	25.053436702237804	25.5872652808046	25.590480559563115
34	24.860212294549072	25.268352703464842	25.750536828479802	24.12089817350629
35	24.80490642308084	25.180478673471367	25.999636319665782	24.01497858378201
36	23.859676004513084	25.709415105410375	25.6890926975635	24.741816192513046
37	24.24730939627526	25.384025818226906	26.043988705602615	24.324676079895216
38	24.05651567779153	24.74980054502821	26.25018191708765	24.943501860092606
39	24.09911427531463	24.60100544382077	26.241520663781227	25.058359617083376
40	24.447918176232353	24.581990685229748	26.761903531176053	24.20818760736185
41	23.291402406136168	24.861719937220528	27.14398325962807	24.70289439701524
42	24.021778359912457	24.84782808689068	27.01174145958887	24.118652093607995
43	23.291987002274077	23.82855395222373	27.08769280519072	25.791766240311475
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	488.0
1	551.0
2	614.0
3	1441.5
4	2269.0
5	2269.0
6	3853.0
7	5437.0
8	5251.0
9	5065.0
10	6941.0
11	8817.0
12	8817.0
13	16855.0
14	24893.0
15	41071.0
16	57249.0
17	50508.5
18	43768.0
19	43768.0
20	46344.5
21	48921.0
22	42313.5
23	35706.0
24	43660.5
25	51615.0
26	51615.0
27	60951.5
28	70288.0
29	79384.5
30	88481.0
31	101791.0
32	115101.0
33	115101.0
34	135095.5
35	155090.0
36	186710.0
37	218330.0
38	251981.0
39	285632.0
40	285632.0
41	317601.5
42	349571.0
43	375050.5
44	400530.0
45	442975.5
46	485421.0
47	485421.0
48	541763.0
49	598105.0
50	606029.5
51	613954.0
52	585371.0
53	556788.0
54	556788.0
55	536929.0
56	517070.0
57	494335.0
58	471600.0
59	438710.5
60	405821.0
61	405821.0
62	344425.5
63	283030.0
64	248913.0
65	214796.0
66	180994.0
67	147192.0
68	147192.0
69	121166.0
70	95140.0
71	79744.5
72	64349.0
73	47914.0
74	31479.0
75	31479.0
76	24799.0
77	18119.0
78	15435.5
79	12752.0
80	10039.0
81	7326.0
82	7326.0
83	5945.5
84	4565.0
85	3820.5
86	3076.0
87	2047.0
88	1018.0
89	1018.0
90	694.5
91	371.0
92	283.5
93	196.0
94	178.5
95	161.0
96	161.0
97	85.5
98	10.0
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	6500214.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.129880221526406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.33924750832394	33.787623616785226
2	10.71174060222969	9.239921782764554
3	3.7816016543179267	4.893000791887749
4	1.805435816061663	3.1147292197757324
5	1.1027467322186104	2.378066723763416
6	0.7541179132802496	1.9515009163610764
7	0.5143250010377721	1.5527942982786889
8	0.4034464844910438	1.3920478841515704
9	0.31526399450336606	1.2237568488981125
>10	2.0081028054741785	16.417031804986973
>50	0.1568585488875906	4.640842769090412
>100	0.0864625774754793	7.179023185189182
>500	0.01096830543461665	3.2989764521661282
>1k	0.009324784814905446	7.242557925936638
>5k	2.5009001416855626E-4	0.6919907308418741
>10k+	1.0718143464366698E-4	0.9961350491227353
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	28520	0.4387547856116737	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	18484	0.2843598687673975	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	17642	0.2714064490799841	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8939	0.13751854938929703	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	8325	0.12807270652935426	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.5384108892414925E-5	0.0	0.0	0.0	6.15364355696597E-5
7	1.5384108892414925E-5	0.0	0.0	0.0	6.15364355696597E-5
8	1.5384108892414925E-5	0.0	0.0	0.0	7.692054446207463E-5
9	1.5384108892414925E-5	0.0	0.0	0.0	9.230465335448956E-5
10	1.5384108892414925E-5	0.0	0.0	0.0	1.0768876224690449E-4
11	1.5384108892414925E-5	0.0	0.0	0.0	1.0768876224690449E-4
12	1.5384108892414925E-5	1.5384108892414925E-5	0.0	3.076821778482985E-5	1.5384108892414926E-4
13	1.5384108892414925E-5	1.5384108892414925E-5	0.0	3.076821778482985E-5	1.5384108892414926E-4
14	1.5384108892414925E-5	3.076821778482985E-5	0.0	7.692054446207463E-5	1.9999341560139405E-4
15	1.5384108892414925E-5	3.076821778482985E-5	0.0	1.0768876224690449E-4	2.461457422786388E-4
16	3.076821778482985E-5	3.076821778482985E-5	0.0	2.307616333862239E-4	2.6152985117105376E-4
17	4.615232667724478E-5	3.076821778482985E-5	0.0	6.769007912662568E-4	2.6152985117105376E-4
18	4.615232667724478E-5	3.076821778482985E-5	0.0	7.845895535131612E-4	3.076821778482985E-4
19	4.615232667724478E-5	3.076821778482985E-5	0.0	0.001046119404684215	3.230662867407135E-4
20	4.615232667724478E-5	3.076821778482985E-5	0.0	0.0014922585625642478	4.15370940095203E-4
21	4.615232667724478E-5	3.076821778482985E-5	0.0	0.002338384551647069	4.4613915788003285E-4
22	4.615232667724478E-5	3.076821778482985E-5	0.0	0.003953715985350636	4.615232667724478E-4
23	4.615232667724478E-5	3.076821778482985E-5	0.0	0.006138259448073556	4.769073756648627E-4
24	4.615232667724478E-5	4.615232667724478E-5	0.0	0.009199697117664126	4.769073756648627E-4
25	4.615232667724478E-5	4.615232667724478E-5	0.0	0.010415041720164905	5.076755934496926E-4
26	4.615232667724478E-5	4.615232667724478E-5	0.0	0.013153413103014763	5.230597023421075E-4
27	4.615232667724478E-5	4.615232667724478E-5	0.0	0.025429931999161874	5.384438112345225E-4
28	4.615232667724478E-5	4.615232667724478E-5	0.0	0.046598465835124815	5.384438112345225E-4
29	4.615232667724478E-5	4.615232667724478E-5	0.0	0.07402833199030062	5.384438112345225E-4
30	4.615232667724478E-5	4.615232667724478E-5	0.0	0.12081140713213442	5.538279201269373E-4
31	4.615232667724478E-5	4.615232667724478E-5	0.0	0.28111382179109795	5.692120290193523E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2595	0.0	16.183044	37
TCGTTAC	940	0.0	16.138298	23
AAGACGG	2240	0.0	16.104912	5
CGTTACG	690	0.0	15.81884	24
CAAGACG	2370	0.0	15.2215185	4
TATACCG	290	0.0	14.672415	5
TCTAGCG	1380	0.0	14.47826	28
CTAGCGG	1370	0.0	14.3138685	29
TCTTATA	4070	0.0	14.272727	37
GTTACGG	1345	0.0	13.754646	25
TACGGAT	1765	0.0	13.626062	27
ACGGACC	2545	0.0	13.59332	8
GACGGAC	2600	0.0	13.5903845	7
TACTTAC	1090	0.0	13.577982	31
ATCTCGT	1350	0.0	13.566668	20
TCTATAC	550	0.0	13.454546	3
TATACTG	635	0.0	13.401575	5
AGACGGA	2755	0.0	13.362977	6
CGCAATA	1510	0.0	13.354303	36
TAGAGTG	1060	0.0	13.264152	5
>>END_MODULE