FastQCFastQC Report
Thu 9 Feb 2017
SRR2727267.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727267.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8605151
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT491360.5710068306761845No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT345750.4017942276666615No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT343570.39926086131434535No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195840.22758461763192767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC56500.018.53274337
AAGACGG19800.017.005055
CGTTACG13750.015.60727224
TCGTTAC15650.015.48562323
CAAGACG22550.015.3414644
TACGGAT28600.014.74825127
ACGGATG30000.014.18333428
GTTACGG24450.014.14928425
TATACTG11200.013.7098225
ATACCGT16650.013.6666676
TTACGGA30750.013.53658526
ACGGACC24300.013.4753088
AGGGCGT22150.013.2799110
TCTTATA88800.013.22916637
ACCGTCG15400.013.0941558
GGCGTCA20500.013.08536612
GGGCGTC20950.013.06921211
GACGGAC25350.012.9901387
TATACCG4000.012.9500015
CGGATGG33400.012.73952129
Produced by FastQC (version 0.11.5)