##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727267.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8605151
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.142014126190233	31.0	31.0	34.0	30.0	34.0
2	31.39331860649511	31.0	31.0	34.0	30.0	34.0
3	31.550213006140158	31.0	31.0	34.0	30.0	34.0
4	35.37547115675251	37.0	35.0	37.0	33.0	37.0
5	35.186433335103594	37.0	35.0	37.0	32.0	37.0
6	35.18872138327381	37.0	35.0	37.0	32.0	37.0
7	35.09489234994249	37.0	35.0	37.0	32.0	37.0
8	35.1018508565393	37.0	35.0	37.0	32.0	37.0
9	36.657084808854606	39.0	35.0	39.0	32.0	39.0
10	36.40312180460285	38.0	35.0	39.0	32.0	39.0
11	36.54101769974751	38.0	35.0	39.0	32.0	39.0
12	36.40330006992323	38.0	35.0	39.0	32.0	39.0
13	36.462827903891515	38.0	35.0	39.0	32.0	39.0
14	37.479040867499016	39.0	36.0	41.0	32.0	41.0
15	37.487285231833816	39.0	36.0	41.0	32.0	41.0
16	37.44135890235976	39.0	36.0	41.0	32.0	41.0
17	37.41463037661977	39.0	36.0	41.0	32.0	41.0
18	37.349026879365624	39.0	36.0	41.0	32.0	41.0
19	37.367346255748444	39.0	36.0	41.0	32.0	41.0
20	37.2735291919921	39.0	36.0	40.0	31.0	41.0
21	37.1686329501946	39.0	36.0	40.0	31.0	41.0
22	37.0921271457061	39.0	36.0	40.0	31.0	41.0
23	36.98875650177435	39.0	36.0	40.0	31.0	41.0
24	36.85498801822304	39.0	36.0	40.0	30.0	41.0
25	36.727975836798215	39.0	35.0	40.0	30.0	41.0
26	36.50308321143929	38.0	35.0	40.0	30.0	41.0
27	36.33335731121976	38.0	35.0	40.0	30.0	41.0
28	36.1286542211752	38.0	35.0	40.0	29.0	41.0
29	36.073018242213294	38.0	35.0	40.0	29.0	41.0
30	35.99052811507898	38.0	34.0	40.0	29.0	41.0
31	36.11801454733334	38.0	35.0	40.0	30.0	41.0
32	36.1045269281155	38.0	35.0	40.0	30.0	41.0
33	36.10010178787101	38.0	35.0	40.0	29.0	41.0
34	36.143796198346784	38.0	35.0	40.0	30.0	41.0
35	35.93923953222901	38.0	35.0	40.0	28.0	41.0
36	35.90173455410602	38.0	35.0	40.0	28.0	41.0
37	35.826394562977455	38.0	34.0	40.0	28.0	41.0
38	35.72995848649257	38.0	34.0	40.0	27.0	41.0
39	35.66614519605757	38.0	34.0	40.0	27.0	41.0
40	35.47783728606273	38.0	34.0	40.0	26.0	41.0
41	35.421439670262615	38.0	34.0	40.0	26.0	41.0
42	35.21533962623085	38.0	34.0	40.0	26.0	41.0
43	34.863120937680236	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	4.0
14	21.0
15	51.0
16	134.0
17	352.0
18	677.0
19	1357.0
20	2907.0
21	5774.0
22	11155.0
23	19656.0
24	32571.0
25	50295.0
26	75481.0
27	108071.0
28	149550.0
29	198629.0
30	257214.0
31	322744.0
32	394376.0
33	478960.0
34	588184.0
35	716857.0
36	885486.0
37	1132350.0
38	1544697.0
39	1627597.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.61510936879551	19.56020295285928	12.96697756959756	25.857710108747657
2	18.376830342663364	22.288870933235224	34.39409720991532	24.940201514186096
3	20.10872325192202	24.031199452513967	30.191823478751274	25.66825381681274
4	15.00726715893771	15.88239416135754	34.29748066013019	34.812858019574556
5	14.505311992781996	37.594738314295704	33.39460283730059	14.50534685562171
6	34.71110501140538	35.306469346092825	15.4428086154444	14.5396170270574
7	28.477710617745117	31.806867770245983	21.199744199724098	18.515677412284806
8	27.597598229246646	34.01144268124987	19.80758966344693	18.583369426056557
9	27.849912221180084	13.624990427245262	19.266855398586266	39.25824195298839
10	16.78379612397272	26.951287664795192	32.73817042838644	23.526745782845644
11	36.62721316569576	21.948783931856628	20.889615998603627	20.534386903843988
12	21.547256985961084	25.41219788008369	29.52661725517658	23.513927878778652
13	30.37197139248341	19.450001516533526	24.95068360799247	25.227343482990594
14	22.709677029490823	20.78740977351821	25.370966761652408	31.131946435338552
15	25.49225458100619	27.789030082098503	22.37580723452732	24.34290810236799
16	24.432412632852113	26.32037485454933	24.81281269788293	24.434399814715626
17	23.767403965369112	26.203851623289353	25.533845948781142	24.494898462560393
18	23.695319233793803	25.25239824379607	26.963919633717058	24.088362888693062
19	24.395899618728365	25.51203343206877	27.164241510695163	22.927825438507703
20	24.034430075660495	24.765794348059668	26.694429882752786	24.50534569352705
21	24.646958548432213	25.54100445186842	26.907383728652757	22.90465327104661
22	24.95784211107975	24.826595140515256	26.058659516840553	24.156903231564446
23	23.638934401034913	25.201440393085488	26.75820563753036	24.401419568349237
24	23.640294051783638	25.53010400398552	26.715800803495487	24.113801140735358
25	24.292566161825633	24.837518830291298	26.239458203580625	24.630456804302447
26	23.740059878089298	25.760803035298274	26.777600997356117	23.721536089256308
27	24.692628868453326	25.559377168395997	25.98150805256061	23.766485910590063
28	23.433545791352177	26.23357800461607	26.401175296052333	23.93170090797942
29	23.654332155240507	26.110000858787952	26.851335903344403	23.38433108262714
30	24.155624927441714	26.052360963799476	26.385428913449633	23.406585195309184
31	24.059879948649364	25.837443177929124	26.3646157981423	23.738061075279212
32	23.479227732319863	25.930817483621148	26.119378962670147	24.47057582138884
33	23.09309854062991	25.441947503303545	26.75488204681126	24.710071909255284
34	24.09313909773344	25.60709277501348	27.08338296445931	23.216385162793774
35	24.06705007268321	25.403168404598595	26.9624205316095	23.567360991108696
36	23.08289534954122	25.800418842156287	27.109960069265487	24.006725739037
37	23.295279769059253	25.409466957639676	27.463980585581822	23.831272687719252
38	23.240231345155944	24.80702546649094	27.55309000388256	24.39965318447056
39	23.015342787128315	24.82305075181133	27.97201350679378	24.18959295426658
40	23.51325386387758	24.658684083521603	28.16637383818134	23.66168821441948
41	22.353820403616393	25.04810200309094	28.605692102323367	23.992385490969305
42	22.78753737151155	25.08100090283134	28.444009872691367	23.68745185296574
43	22.01458173133743	24.44432410308663	28.350077761563973	25.191016404011968
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3034.0
1	3162.0
2	3290.0
3	5381.0
4	7472.0
5	7472.0
6	11151.5
7	14831.0
8	13818.0
9	12805.0
10	17579.5
11	22354.0
12	22354.0
13	38735.5
14	55117.0
15	79541.0
16	103965.0
17	92271.0
18	80577.0
19	80577.0
20	84659.0
21	88741.0
22	75042.5
23	61344.0
24	72734.5
25	84125.0
26	84125.0
27	98315.0
28	112505.0
29	126085.5
30	139666.0
31	158876.5
32	178087.0
33	178087.0
34	208050.0
35	238013.0
36	280413.5
37	322814.0
38	365604.0
39	408394.0
40	408394.0
41	447798.0
42	487202.0
43	520174.0
44	553146.0
45	601824.0
46	650502.0
47	650502.0
48	699977.5
49	749453.0
50	758674.0
51	767895.0
52	746015.0
53	724135.0
54	724135.0
55	690739.0
56	657343.0
57	621959.0
58	586575.0
59	536774.0
60	486973.0
61	486973.0
62	410955.0
63	334937.0
64	290037.5
65	245138.0
66	204787.0
67	164436.0
68	164436.0
69	135241.5
70	106047.0
71	87967.5
72	69888.0
73	51963.5
74	34039.0
75	34039.0
76	26855.0
77	19671.0
78	16630.5
79	13590.0
80	10574.0
81	7558.0
82	7558.0
83	6122.0
84	4686.0
85	3871.5
86	3057.0
87	2008.0
88	959.0
89	959.0
90	677.0
91	395.0
92	292.0
93	189.0
94	188.5
95	188.0
96	188.0
97	97.5
98	7.0
99	7.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	8605151.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.44291728362496
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.32992551891722	35.595403261886595
2	10.65470132913954	9.68361422363634
3	3.803397467104853	5.185124294831835
4	1.9429714476111133	3.5317716311294745
5	1.1651554501968806	2.647403137293083
6	0.7497615309530008	2.0442810740124733
7	0.547957629796724	1.7430555272048602
8	0.38124498271176976	1.3859907371334383
9	0.32355289940296367	1.3232868880001314
>10	1.872367958512528	15.879044202924561
>50	0.13908073784362024	4.329986028497582
>100	0.07475294694245126	6.466849248600523
>500	0.008360550344163697	2.701287280004955
>1k	0.006564412637287665	5.589323261338887
>5k	1.0256894293307144E-4	0.2895782649324086
>10k+	1.0256894293307144E-4	1.604000938572931
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	49136	0.5710068306761845	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	34575	0.4017942276666615	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	34357	0.39926086131434535	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19584	0.22758461763192767	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1620946570257744E-5
2	0.0	0.0	0.0	1.1620946570257744E-5	1.1620946570257744E-5
3	0.0	0.0	0.0	1.1620946570257744E-5	1.1620946570257744E-5
4	0.0	0.0	0.0	2.3241893140515488E-5	1.1620946570257744E-5
5	0.0	0.0	0.0	2.3241893140515488E-5	1.1620946570257744E-5
6	0.0	0.0	1.1620946570257744E-5	3.486283971077323E-5	5.810473285128872E-5
7	0.0	0.0	1.1620946570257744E-5	4.6483786281030975E-5	6.972567942154646E-5
8	0.0	0.0	1.1620946570257744E-5	4.6483786281030975E-5	1.045885191323197E-4
9	0.0	0.0	1.1620946570257744E-5	5.810473285128872E-5	1.3945135884309293E-4
10	0.0	0.0	1.1620946570257744E-5	5.810473285128872E-5	1.3945135884309293E-4
11	0.0	0.0	1.1620946570257744E-5	6.972567942154646E-5	1.3945135884309293E-4
12	0.0	0.0	1.1620946570257744E-5	1.045885191323197E-4	1.3945135884309293E-4
13	0.0	0.0	1.1620946570257744E-5	1.278304122728352E-4	1.5107230541335068E-4
14	0.0	0.0	1.1620946570257744E-5	1.9755609169438165E-4	2.556608245456704E-4
15	0.0	0.0	1.1620946570257744E-5	2.3241893140515488E-4	2.9052366425644363E-4
16	0.0	0.0	1.1620946570257744E-5	5.578054353723717E-4	3.370074505374746E-4
17	0.0	0.0	1.1620946570257744E-5	7.902243667775266E-4	3.4862839710773235E-4
18	0.0	0.0	1.1620946570257744E-5	9.180547790503618E-4	3.951121833887633E-4
19	0.0	0.0	1.1620946570257744E-5	0.001092368977604228	4.183540765292788E-4
20	0.0	1.1620946570257744E-5	1.1620946570257744E-5	0.0014409973747119603	4.183540765292788E-4
21	0.0	1.1620946570257744E-5	1.1620946570257744E-5	0.0020452865963653632	4.5321691624005203E-4
22	0.0	1.1620946570257744E-5	1.1620946570257744E-5	0.003126034627399333	4.6483786281030975E-4
23	0.0	1.1620946570257744E-5	1.1620946570257744E-5	0.0047181043075246445	5.810473285128873E-4
24	0.0	1.1620946570257744E-5	1.1620946570257744E-5	0.006984188888724904	5.926682750831449E-4
25	0.0	1.1620946570257744E-5	1.1620946570257744E-5	0.008099799759469648	5.926682750831449E-4
26	0.0	1.1620946570257744E-5	1.1620946570257744E-5	0.011446632371703878	5.926682750831449E-4
27	0.0	1.1620946570257744E-5	1.1620946570257744E-5	0.032991867312961734	6.042892216534027E-4
28	0.0	1.1620946570257744E-5	1.1620946570257744E-5	0.07414163911824441	6.042892216534027E-4
29	0.0	1.1620946570257744E-5	1.1620946570257744E-5	0.13064268134283757	6.042892216534027E-4
30	0.0	1.1620946570257744E-5	1.1620946570257744E-5	0.21990317194898729	6.159101682236605E-4
31	0.0	1.1620946570257744E-5	1.1620946570257744E-5	0.5134366613671276	6.507730079344337E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	5650	0.0	18.532743	37
AAGACGG	1980	0.0	17.00505	5
CGTTACG	1375	0.0	15.607272	24
TCGTTAC	1565	0.0	15.485623	23
CAAGACG	2255	0.0	15.341464	4
TACGGAT	2860	0.0	14.748251	27
ACGGATG	3000	0.0	14.183334	28
GTTACGG	2445	0.0	14.149284	25
TATACTG	1120	0.0	13.709822	5
ATACCGT	1665	0.0	13.666667	6
TTACGGA	3075	0.0	13.536585	26
ACGGACC	2430	0.0	13.475308	8
AGGGCGT	2215	0.0	13.27991	10
TCTTATA	8880	0.0	13.229166	37
ACCGTCG	1540	0.0	13.094155	8
GGCGTCA	2050	0.0	13.085366	12
GGGCGTC	2095	0.0	13.069212	11
GACGGAC	2535	0.0	12.990138	7
TATACCG	400	0.0	12.950001	5
CGGATGG	3340	0.0	12.739521	29
>>END_MODULE