FastQCFastQC Report
Thu 9 Feb 2017
SRR2727266.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727266.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4111375
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT166780.40565504241281813No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT114070.27744975829254204No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT109000.26511811741813873No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61930.15063087166702135No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT58740.14287191024900428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG13550.019.1143915
CAAGACG15200.018.1348694
CGCTTCG9500.015.96842132
GTAAACG10250.015.16097627
CTTATAC19350.015.10594437
TAAACGC10550.015.08056928
GACGGAC16900.014.7781087
ACGGACC17250.014.5855068
CGCAAGA18150.014.37190152
AGACGGA18300.014.1530066
CACTCTA11450.013.8951979
GCGCAAG18300.013.8497281
CGGACCA18750.013.8133339
TCTTATA30550.013.74631837
ACGTTAC950.00124602913.63157931
TTCGGGC11400.013.63157935
AAACGCT12200.013.49590229
TCTAGCG8550.013.41520528
CTAGCGG9000.013.36111229
AACGCTT11450.013.24890930
Produced by FastQC (version 0.11.5)