##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727266.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4111375
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.136840108236296	31.0	31.0	33.0	30.0	34.0
2	31.383733179289166	31.0	31.0	34.0	30.0	34.0
3	31.53539460642729	31.0	31.0	34.0	30.0	34.0
4	35.358960201878936	37.0	35.0	37.0	33.0	37.0
5	35.160736979720895	37.0	35.0	37.0	32.0	37.0
6	35.15948849229272	37.0	35.0	37.0	32.0	37.0
7	35.060328722142835	37.0	35.0	37.0	32.0	37.0
8	35.062911799580434	37.0	35.0	37.0	32.0	37.0
9	36.62919363959746	39.0	35.0	39.0	32.0	39.0
10	36.3607338177617	38.0	35.0	39.0	32.0	39.0
11	36.51226244261348	38.0	35.0	39.0	32.0	39.0
12	36.36082551457846	38.0	35.0	39.0	32.0	39.0
13	36.425910309811194	38.0	35.0	39.0	32.0	39.0
14	37.44390964093521	39.0	36.0	41.0	32.0	41.0
15	37.44580365449515	39.0	36.0	41.0	32.0	41.0
16	37.39760347815512	39.0	36.0	41.0	32.0	41.0
17	37.350319053844515	39.0	36.0	41.0	32.0	41.0
18	37.27600085129671	39.0	36.0	41.0	31.0	41.0
19	37.28294816211122	39.0	36.0	41.0	31.0	41.0
20	37.18090930649722	39.0	36.0	40.0	31.0	41.0
21	37.06870913015719	39.0	36.0	40.0	31.0	41.0
22	36.99108424797057	39.0	36.0	40.0	31.0	41.0
23	36.88142361132225	39.0	36.0	40.0	30.0	41.0
24	36.73761551792283	39.0	35.0	40.0	30.0	41.0
25	36.60563558420237	38.0	35.0	40.0	30.0	41.0
26	36.37580128302575	38.0	35.0	40.0	30.0	41.0
27	36.19831564865769	38.0	35.0	40.0	29.0	41.0
28	35.976106047246965	38.0	34.0	40.0	28.0	41.0
29	35.91927834361984	38.0	34.0	40.0	28.0	41.0
30	35.83987789972941	38.0	34.0	40.0	28.0	41.0
31	36.000714116323614	38.0	34.0	40.0	29.0	41.0
32	35.99452591894439	38.0	35.0	40.0	29.0	41.0
33	35.98974965796114	38.0	35.0	40.0	29.0	41.0
34	36.0425949955915	38.0	35.0	40.0	29.0	41.0
35	35.82766884558086	38.0	34.0	40.0	27.0	41.0
36	35.795247818552184	38.0	34.0	40.0	27.0	41.0
37	35.71957216259767	38.0	34.0	40.0	27.0	41.0
38	35.621259311057734	38.0	34.0	40.0	27.0	41.0
39	35.554161807181295	38.0	34.0	40.0	27.0	41.0
40	35.361427989419596	38.0	34.0	40.0	26.0	41.0
41	35.29626609102794	38.0	34.0	40.0	26.0	41.0
42	35.08503408227175	38.0	33.0	40.0	25.0	41.0
43	34.735089355750816	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	4.0
14	11.0
15	24.0
16	116.0
17	197.0
18	378.0
19	763.0
20	1659.0
21	3135.0
22	5880.0
23	10161.0
24	16735.0
25	25982.0
26	38372.0
27	53909.0
28	74437.0
29	98740.0
30	126513.0
31	157320.0
32	192592.0
33	233247.0
34	284495.0
35	343969.0
36	422663.0
37	537330.0
38	736641.0
39	746101.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.68009485877596	19.716712778571647	12.621081754887356	25.98211060776504
2	19.034872761545714	22.213347116232406	33.331598309567966	25.42018181265392
3	20.271977136602718	23.755653522240127	29.943850901462405	26.028518439694746
4	15.033316104709495	15.777349426894896	34.34077407193457	34.84856039646104
5	14.77602991699857	37.1574716487793	33.35395092882551	14.712547505396614
6	34.98343011766137	35.3420206135417	15.267665926849288	14.406883341947646
7	28.961259919126814	31.358584415189565	20.984196284697944	18.69595938098568
8	27.851460885956648	33.20015809795993	19.745754157672312	19.202626858411115
9	27.727293180505306	13.35776960262686	19.148897874798575	39.76603934206926
10	16.982299109178804	26.330120701711714	32.609820315587854	24.077759873521632
11	37.167371013347115	21.58316864795841	20.752619257547657	20.49684108114682
12	21.76354625885501	25.187923748137784	28.96186798820346	24.086662004803745
13	30.41612599191268	19.051728436350366	24.692469064485724	25.839676507251223
14	23.013882217019855	20.717740415311177	24.50421087835578	31.764166489313183
15	25.750290352984102	27.703359581648474	21.67895168891186	24.867398376455565
16	25.159903925085892	25.92628986652884	24.05158858046274	24.86221762792253
17	24.259888723358973	25.902794077407194	24.888170016113833	24.94914718312
18	24.347864157368278	24.651582499771973	26.336201392478188	24.664351950381562
19	24.802286339728195	25.22214587577149	26.260387340001824	23.715180444498497
20	24.564604299048373	24.483731111854308	25.72263537137819	25.229029217719134
21	25.440539965340065	25.252841202760635	25.80051685871515	23.506101973184155
22	25.532504332492174	24.56713386640722	25.236569274269556	24.66379252683105
23	24.134358943175947	24.727323583959137	25.90145632543857	25.23686114742635
24	24.239603538962026	25.334711623240402	25.77682648748898	24.648858350308593
25	24.904612203946368	24.518342403697062	25.342592198473746	25.23445319388283
26	24.337989115563527	25.713781885622204	25.75936274360767	24.18886625520659
27	25.42672463591864	25.12242254720136	25.09822139795081	24.352631418929192
28	24.10916056063969	25.72582165333982	25.71446292298805	24.45055486303244
29	24.18154510352376	25.742920555775132	26.00820893253474	24.06732540816637
30	24.456562585509715	25.79703870359673	25.739247818552187	24.007150892341368
31	24.671843361405855	25.620795962421333	25.282685233042475	24.42467544313034
32	24.14547444589705	25.504557477729474	25.14932352315223	25.200644553221245
33	23.65155209631814	25.079565838679272	25.810708096439754	25.458173968562832
34	24.814131525341278	25.278720622662735	26.040631175701563	23.866516676294427
35	24.91570338390441	25.069082727797877	26.11678574686084	23.898428141436867
36	23.646614575415768	25.75046061232556	26.043744489373992	24.55918032288468
37	24.14413669392843	25.2838040801435	26.362397008300142	24.209662217627923
38	23.777300781368762	24.66413304551397	26.6932352315223	24.865330941594966
39	23.97086132984707	24.573044297832233	26.686084339180933	24.770010033139762
40	24.183296342464505	24.51926666869356	27.183801039798123	24.11363594904381
41	23.035383539570095	24.870730594995592	27.63335866954486	24.460527195889455
42	23.827527287099816	24.81996898847709	27.44291143473899	23.90959228968411
43	23.003763947584446	24.029090024626797	27.425496336383816	25.541649691404945
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	622.0
1	693.5
2	765.0
3	1392.5
4	2020.0
5	2020.0
6	3081.0
7	4142.0
8	3991.0
9	3840.0
10	5367.0
11	6894.0
12	6894.0
13	12585.0
14	18276.0
15	27218.5
16	36161.0
17	32202.0
18	28243.0
19	28243.0
20	30603.0
21	32963.0
22	29265.0
23	25567.0
24	30542.0
25	35517.0
26	35517.0
27	42140.0
28	48763.0
29	55209.0
30	61655.0
31	70407.5
32	79160.0
33	79160.0
34	93444.0
35	107728.0
36	129121.5
37	150515.0
38	171686.0
39	192857.0
40	192857.0
41	212381.5
42	231906.0
43	246472.0
44	261038.0
45	284123.0
46	307208.0
47	307208.0
48	331427.0
49	355646.0
50	359804.0
51	363962.0
52	355213.5
53	346465.0
54	346465.0
55	334220.0
56	321975.0
57	307821.5
58	293668.0
59	272914.5
60	252161.0
61	252161.0
62	212558.0
63	172955.0
64	151681.5
65	130408.0
66	110060.5
67	89713.0
68	89713.0
69	73910.0
70	58107.0
71	49220.5
72	40334.0
73	29988.5
74	19643.0
75	19643.0
76	15189.0
77	10735.0
78	9422.5
79	8110.0
80	6506.5
81	4903.0
82	4903.0
83	4002.5
84	3102.0
85	2708.0
86	2314.0
87	1536.5
88	759.0
89	759.0
90	524.0
91	289.0
92	219.5
93	150.0
94	137.5
95	125.0
96	125.0
97	66.0
98	7.0
99	5.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4111375.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.21852286784417
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.30872676958548	41.33422677501566
2	8.878713445103651	8.917517483599463
3	3.118766642242017	4.698595618287008
4	1.5063382944013424	3.0258433633641277
5	0.9079070467461753	2.2796875394449834
6	0.5999174326593727	1.8076180386493845
7	0.41633633573552226	1.4635457057794141
8	0.33280778654316123	1.3370492351291474
9	0.246829474836673	1.1155870463889086
>10	1.5024878237334858	13.806633712715788
>50	0.09902338823080073	3.42522122207093
>100	0.06449039556958346	6.517097683341133
>500	0.01125274233269266	3.9651964923575838
>1k	0.00615990628449592	5.0626763926976315
>5k	9.700639818103801E-5	0.2939230400614021
>10k+	1.45509597271557E-4	0.9495806510975189
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	16678	0.40565504241281813	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11407	0.27744975829254204	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10900	0.26511811741813873	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6193	0.15063087166702135	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	5874	0.14287191024900428	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.4322763065884285E-5
2	0.0	0.0	0.0	0.0	2.4322763065884285E-5
3	0.0	0.0	0.0	0.0	2.4322763065884285E-5
4	0.0	0.0	0.0	0.0	2.4322763065884285E-5
5	0.0	2.4322763065884285E-5	0.0	0.0	2.4322763065884285E-5
6	0.0	2.4322763065884285E-5	0.0	0.0	4.864552613176857E-5
7	0.0	2.4322763065884285E-5	0.0	0.0	4.864552613176857E-5
8	0.0	2.4322763065884285E-5	0.0	0.0	4.864552613176857E-5
9	0.0	2.4322763065884285E-5	0.0	2.4322763065884285E-5	7.296828919765286E-5
10	0.0	2.4322763065884285E-5	0.0	2.4322763065884285E-5	7.296828919765286E-5
11	0.0	2.4322763065884285E-5	0.0	2.4322763065884285E-5	7.296828919765286E-5
12	0.0	2.4322763065884285E-5	0.0	2.4322763065884285E-5	9.729105226353714E-5
13	0.0	2.4322763065884285E-5	0.0	2.4322763065884285E-5	9.729105226353714E-5
14	0.0	2.4322763065884285E-5	0.0	7.296828919765286E-5	9.729105226353714E-5
15	0.0	2.4322763065884285E-5	0.0	1.7025934146119E-4	1.2161381532942143E-4
16	0.0	2.4322763065884285E-5	0.0	6.080690766471071E-4	1.459365783953057E-4
17	0.0	2.4322763065884285E-5	0.0	8.756194703718343E-4	1.459365783953057E-4
18	0.0	2.4322763065884285E-5	0.0	9.485877595694871E-4	1.7025934146119E-4
19	0.0	2.4322763065884285E-5	0.0	0.0010458788118330243	1.7025934146119E-4
20	0.0	2.4322763065884285E-5	0.0	0.0013134292055577514	1.7025934146119E-4
21	0.0	2.4322763065884285E-5	0.0	0.0017512389407436685	1.9458210452707428E-4
22	0.0	2.4322763065884285E-5	0.0	0.0031133136724331885	2.1890486759295857E-4
23	0.0	2.4322763065884285E-5	0.0	0.004645647745583899	3.4051868292238E-4
24	0.0	2.4322763065884285E-5	0.0	0.006931987473777021	3.4051868292238E-4
25	0.0	2.4322763065884285E-5	0.0	0.007977866285610046	3.8916420905414856E-4
26	0.0	2.4322763065884285E-5	0.0	0.011188471010306771	3.8916420905414856E-4
27	0.0	2.4322763065884285E-5	0.0	0.026098324769693836	4.134869721200328E-4
28	0.0	2.4322763065884285E-5	0.0	0.05134535283208173	4.134869721200328E-4
29	0.0	2.4322763065884285E-5	0.0	0.0889726672950047	4.134869721200328E-4
30	0.0	2.4322763065884285E-5	0.0	0.14773646286218114	4.3780973518591713E-4
31	0.0	2.4322763065884285E-5	0.0	0.34596698184913804	4.621324982518014E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	1355	0.0	19.114391	5
CAAGACG	1520	0.0	18.134869	4
CGCTTCG	950	0.0	15.968421	32
GTAAACG	1025	0.0	15.160976	27
CTTATAC	1935	0.0	15.105944	37
TAAACGC	1055	0.0	15.080569	28
GACGGAC	1690	0.0	14.778108	7
ACGGACC	1725	0.0	14.585506	8
CGCAAGA	1815	0.0	14.3719015	2
AGACGGA	1830	0.0	14.153006	6
CACTCTA	1145	0.0	13.895197	9
GCGCAAG	1830	0.0	13.849728	1
CGGACCA	1875	0.0	13.813333	9
TCTTATA	3055	0.0	13.746318	37
ACGTTAC	95	0.001246029	13.631579	31
TTCGGGC	1140	0.0	13.631579	35
AAACGCT	1220	0.0	13.495902	29
TCTAGCG	855	0.0	13.415205	28
CTAGCGG	900	0.0	13.361112	29
AACGCTT	1145	0.0	13.248909	30
>>END_MODULE