FastQCFastQC Report
Thu 9 Feb 2017
SRR2727265.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727265.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4612114
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT189920.41178513800829725No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT138670.300664727714883No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT134020.2905825831711879No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77380.16777555801959795No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT64280.13937209704703743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC851.2469736E-619.5882363
CTTATAC28300.018.89222537
AAGACGG14300.017.5944065
CAAGACG14650.016.9215014
AGCGTTA855.368504E-415.23529410
GACGGAC17350.014.6080697
TATACTG4150.014.265065
ACGGACC17700.014.1101698
TCTTATA45600.013.9561437
GCGCAAG18700.013.9491971
TTCGCGA800.006302415413.87500122
TCGTTAC8200.013.76219623
CGCAAGA18750.013.6159992
AGACGGA18500.013.66
GTAAACG12000.013.56666827
ACCGTCG13300.013.4924818
TACCGTC13200.013.4545447
TCTAGCG11100.013.33333328
CTAGCGG10750.013.25116329
CGGACCA18900.013.2142869
Produced by FastQC (version 0.11.5)