##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727265.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4612114
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.214190932834704	31.0	31.0	34.0	30.0	34.0
2	31.464101277635375	31.0	31.0	34.0	30.0	34.0
3	31.621922181455187	31.0	31.0	34.0	30.0	34.0
4	35.43141691640753	37.0	35.0	37.0	33.0	37.0
5	35.24980930653492	37.0	35.0	37.0	33.0	37.0
6	35.24963975305034	37.0	35.0	37.0	32.0	37.0
7	35.15619973834124	37.0	35.0	37.0	32.0	37.0
8	35.15781374874949	37.0	35.0	37.0	32.0	37.0
9	36.734613888555224	39.0	35.0	39.0	32.0	39.0
10	36.472430646770654	38.0	35.0	39.0	32.0	39.0
11	36.61859962698233	39.0	35.0	39.0	32.0	39.0
12	36.475390027219625	38.0	35.0	39.0	32.0	39.0
13	36.53782690540607	38.0	35.0	39.0	32.0	39.0
14	37.57338933946559	39.0	36.0	41.0	32.0	41.0
15	37.57966628752021	39.0	36.0	41.0	32.0	41.0
16	37.52868727876197	39.0	36.0	41.0	32.0	41.0
17	37.485636738380705	39.0	36.0	41.0	32.0	41.0
18	37.413638518041836	39.0	36.0	41.0	32.0	41.0
19	37.424721938789894	39.0	36.0	41.0	32.0	41.0
20	37.32870154553855	39.0	36.0	41.0	31.0	41.0
21	37.21916262260647	39.0	36.0	40.0	31.0	41.0
22	37.13895840389028	39.0	36.0	40.0	31.0	41.0
23	37.0223040887541	39.0	36.0	40.0	31.0	41.0
24	36.87897133505373	39.0	36.0	40.0	30.0	41.0
25	36.74851727429114	39.0	35.0	40.0	30.0	41.0
26	36.530842472670884	38.0	35.0	40.0	30.0	41.0
27	36.35983022102229	38.0	35.0	40.0	30.0	41.0
28	36.12785546931407	38.0	35.0	40.0	29.0	41.0
29	36.06375579614901	38.0	35.0	40.0	29.0	41.0
30	35.98113207956265	38.0	34.0	40.0	29.0	41.0
31	36.1352991274717	38.0	35.0	40.0	30.0	41.0
32	36.12947793571451	38.0	35.0	40.0	29.0	41.0
33	36.12018913669523	38.0	35.0	40.0	29.0	41.0
34	36.171714532641644	38.0	35.0	40.0	29.0	41.0
35	35.95572659305473	38.0	35.0	40.0	28.0	41.0
36	35.923301115280324	38.0	35.0	40.0	28.0	41.0
37	35.85325579549855	38.0	34.0	40.0	28.0	41.0
38	35.75828437892038	38.0	34.0	40.0	27.0	41.0
39	35.68862131335002	38.0	34.0	40.0	27.0	41.0
40	35.49699812276973	38.0	34.0	40.0	26.0	41.0
41	35.43292728670627	38.0	34.0	40.0	26.0	41.0
42	35.2312085087229	38.0	34.0	40.0	26.0	41.0
43	34.87526284042415	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	3.0
14	7.0
15	33.0
16	113.0
17	195.0
18	421.0
19	802.0
20	1668.0
21	3241.0
22	6126.0
23	10429.0
24	17262.0
25	26830.0
26	39102.0
27	56331.0
28	78110.0
29	104041.0
30	134462.0
31	168786.0
32	207884.0
33	253739.0
34	313746.0
35	381573.0
36	473895.0
37	605764.0
38	834653.0
39	892895.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.65285159907149	19.503160589699213	12.782771631403733	26.061216179825564
2	18.707104811372833	21.944275445056217	34.13144167728725	25.2171780662837
3	20.136622815481147	23.69824336519002	29.959623721356408	26.20551009797243
4	14.913616619190245	15.715634956117736	34.27911799231328	35.09163043237873
5	14.741049332258482	37.21274018812198	33.262382499652006	14.783827979967537
6	34.90982660012307	35.11251022849826	15.44239799796796	14.53526517341072
7	28.806768436339603	31.48712282480442	21.103944958862684	18.602163779993298
8	27.504068633169084	33.61358804227302	19.875137518283374	19.00720580627452
9	27.82543970075328	13.552635515947783	19.253166769078128	39.36875801422081
10	17.059877531214536	26.288530595731157	32.41006185016242	24.241530022891887
11	37.093619108287434	21.597579764940765	20.78372303893616	20.525078087835645
12	21.71262462289527	25.12305636851127	29.031220824116666	24.133098184476793
13	30.24094807717242	19.07949369855125	24.876336534612978	25.803221689663353
14	22.96421987834646	20.856964940589066	24.551387931868117	31.627427249196355
15	25.822843927968826	27.713408645146238	21.7879913636133	24.675756063271635
16	24.968159937070073	25.96147883595245	24.380837073845097	24.689524153132382
17	24.228282301781785	25.902286890566884	24.889562573691805	24.97986823395952
18	24.310370472195615	24.72690397505352	26.490195168636333	24.47253038411453
19	24.842382473633563	25.305380569517578	26.257243424598787	23.59499353225007
20	24.463402248947013	24.354883682406808	25.825727638128633	25.355986430517547
21	25.482826313486612	25.30093575310584	25.996885593027407	23.21935234038014
22	25.483368364268532	24.39200331995263	25.38796742665077	24.736660889128064
23	23.932040708447364	24.707606967217203	26.20121705577963	25.15913526855581
24	24.01807500855356	25.277866071827365	26.06644154936326	24.63761737025581
25	24.82158940563915	24.473419347396877	25.47280921503675	25.232182031927223
26	24.14109885401792	25.683840425453493	26.064772032954952	24.11028868757364
27	25.556068215139522	25.067029999692114	25.207291060021497	24.169610725146864
28	23.963457971767394	25.82468690062735	25.832991118606348	24.37886400899891
29	24.01172217338947	25.792250581837312	26.26491886367076	23.93110838110246
30	24.52144504667491	25.820480586559658	25.83537614204679	23.822698224718643
31	24.607414300687278	25.58197824251525	25.477687672074023	24.332919784723448
32	24.08541939769919	25.453945847825963	25.199745713137183	25.26088904133766
33	23.56411831971196	24.855500102556007	26.034894193855575	25.54548738387646
34	24.84227406347718	25.154994000581947	26.31038608325813	23.692345852682738
35	24.832018462683273	24.83505394706202	26.406589256033136	23.926338334221576
36	23.45143680316662	25.54234348934133	26.458105762346722	24.548113945145328
37	23.908645796699734	25.11692035365995	26.754390719743704	24.220043129896617
38	23.732847887107734	24.29068318779631	27.024960788046435	24.95150813704952
39	23.753077222288955	24.234678501008432	27.183521482773408	24.828722793929206
40	24.165491139204278	24.1583794329455	27.640405245837375	24.035724182012846
41	22.722833824142246	24.76142176884613	28.165934319923576	24.34981008708805
42	23.606615101014416	24.712940746911286	27.87706461722325	23.803379534851047
43	22.57112465129873	23.955890942851806	27.771950129593503	25.701034276255964
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1261.0
1	1332.0
2	1403.0
3	2397.0
4	3391.0
5	3391.0
6	4813.0
7	6235.0
8	5834.5
9	5434.0
10	7479.0
11	9524.0
12	9524.0
13	16072.5
14	22621.0
15	32280.0
16	41939.0
17	37863.0
18	33787.0
19	33787.0
20	36139.5
21	38492.0
22	33815.5
23	29139.0
24	34841.5
25	40544.0
26	40544.0
27	47191.5
28	53839.0
29	61115.0
30	68391.0
31	77973.5
32	87556.0
33	87556.0
34	103403.5
35	119251.0
36	143818.5
37	168386.0
38	191825.5
39	215265.0
40	215265.0
41	237053.5
42	258842.0
43	274785.5
44	290729.0
45	319633.5
46	348538.0
47	348538.0
48	377284.5
49	406031.0
50	409977.0
51	413923.0
52	401811.0
53	389699.0
54	389699.0
55	372598.0
56	355497.0
57	340666.5
58	325836.0
59	301514.0
60	277192.0
61	277192.0
62	232497.0
63	187802.0
64	166049.0
65	144296.0
66	121942.0
67	99588.0
68	99588.0
69	82233.5
70	64879.0
71	55177.0
72	45475.0
73	33570.5
74	21666.0
75	21666.0
76	17019.5
77	12373.0
78	10802.0
79	9231.0
80	7519.0
81	5807.0
82	5807.0
83	4772.5
84	3738.0
85	3329.0
86	2920.0
87	1910.0
88	900.0
89	900.0
90	629.5
91	359.0
92	259.5
93	160.0
94	159.5
95	159.0
96	159.0
97	84.5
98	10.0
99	8.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4612114.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.96467743037247
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.82956306021312	40.06758159511658
2	9.284641746344011	9.092389763326093
3	3.135543064608279	4.605925641827578
4	1.5341216909733864	3.004710949497977
5	0.9531190745656389	2.3334584019420817
6	0.6207281988664828	1.8236253617660052
7	0.4434688725069972	1.5200017204901264
8	0.3392252910692134	1.3288045562742594
9	0.2358957132266068	1.0395501754813605
>10	1.4430106900122275	12.859659452295357
>50	0.09457834702423286	3.2003542913106426
>100	0.06619139088744952	6.725680561469677
>500	0.01090994124989086	3.745058258896103
>1k	0.008781171692619856	7.340995716033101
>5k	8.869870396585521E-5	0.3076220158180035
>10k+	1.3304805594878283E-4	1.004581538455221
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	18992	0.41178513800829725	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13867	0.300664727714883	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13402	0.2905825831711879	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7738	0.16777555801959795	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	6428	0.13937209704703743	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	4.3364062553527516E-5
5	0.0	0.0	0.0	0.0	4.3364062553527516E-5
6	0.0	0.0	0.0	0.0	6.504609383029127E-5
7	0.0	0.0	0.0	0.0	6.504609383029127E-5
8	0.0	0.0	0.0	0.0	8.672812510705503E-5
9	0.0	0.0	0.0	0.0	8.672812510705503E-5
10	0.0	0.0	0.0	0.0	8.672812510705503E-5
11	0.0	0.0	0.0	2.1682031276763758E-5	8.672812510705503E-5
12	0.0	0.0	0.0	4.3364062553527516E-5	1.0841015638381879E-4
13	0.0	0.0	0.0	8.672812510705503E-5	1.0841015638381879E-4
14	0.0	0.0	0.0	8.672812510705503E-5	1.517742189373463E-4
15	0.0	0.0	0.0	1.9513828149087382E-4	2.3850234404440134E-4
16	2.1682031276763758E-5	0.0	0.0	4.3364062553527516E-4	2.8186640659792886E-4
17	2.1682031276763758E-5	0.0	0.0	8.239171885170228E-4	2.8186640659792886E-4
18	2.1682031276763758E-5	0.0	0.0	9.756914074543691E-4	3.252304691514564E-4
19	2.1682031276763758E-5	0.0	0.0	0.0012141937514987705	3.252304691514564E-4
20	2.1682031276763758E-5	0.0	0.0	0.001431014064266408	3.4691250042822013E-4
21	2.1682031276763758E-5	0.0	0.0	0.002146521096399612	4.119585942585114E-4
22	2.1682031276763758E-5	0.0	0.0	0.0033390328166216184	4.119585942585114E-4
23	2.1682031276763758E-5	0.0	0.0	0.005160323443869774	4.770046880888027E-4
24	2.1682031276763758E-5	0.0	0.0	0.0078055312596349525	4.770046880888027E-4
25	2.1682031276763758E-5	0.0	0.0	0.008932996886026667	4.986867193655664E-4
26	2.1682031276763758E-5	0.0	0.0	0.012944172672227963	4.986867193655664E-4
27	2.1682031276763758E-5	0.0	0.0	0.030983622694495408	5.203687506423302E-4
28	2.1682031276763758E-5	0.0	0.0	0.06743111727073528	5.420507819190939E-4
29	2.1682031276763758E-5	0.0	0.0	0.11814538842708572	5.420507819190939E-4
30	2.1682031276763758E-5	0.0	0.0	0.20027692290346683	5.420507819190939E-4
31	2.1682031276763758E-5	0.0	0.0	0.49482731779830247	5.420507819190939E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAC	85	1.2469736E-6	19.588236	3
CTTATAC	2830	0.0	18.892225	37
AAGACGG	1430	0.0	17.594406	5
CAAGACG	1465	0.0	16.921501	4
AGCGTTA	85	5.368504E-4	15.235294	10
GACGGAC	1735	0.0	14.608069	7
TATACTG	415	0.0	14.26506	5
ACGGACC	1770	0.0	14.110169	8
TCTTATA	4560	0.0	13.95614	37
GCGCAAG	1870	0.0	13.949197	1
TTCGCGA	80	0.0063024154	13.875001	22
TCGTTAC	820	0.0	13.762196	23
CGCAAGA	1875	0.0	13.615999	2
AGACGGA	1850	0.0	13.6	6
GTAAACG	1200	0.0	13.566668	27
ACCGTCG	1330	0.0	13.492481	8
TACCGTC	1320	0.0	13.454544	7
TCTAGCG	1110	0.0	13.333333	28
CTAGCGG	1075	0.0	13.251163	29
CGGACCA	1890	0.0	13.214286	9
>>END_MODULE