FastQCFastQC Report
Thu 9 Feb 2017
SRR2727264.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727264.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7387323
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT112540.1523420594984137No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT96750.13096760490911252No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT82590.11179963296582539No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39450.020.2585561
TAGTACT8100.014.1604944
TATACTG15050.013.8903655
TCTTATA33850.013.00738637
AAGACGG11700.012.9658135
TACCGTG6700.012.7014927
GTATCAA63100.012.5776552
GTATTAG12650.012.5770751
ATACCGT11650.012.3862676
GTATAAG12250.012.3836741
ATTATAC10950.012.3333343
GTACATG132450.012.3333331
CTTATAC23750.012.30736937
GTACTAG5150.012.2135921
TACATGG136000.011.8889692
GTTACGG12150.011.87654325
ACGGACC12000.011.8708338
TATACCG4400.011.7727285
TTATACT13950.011.6702514
TTAGTAC4500.011.5111113
Produced by FastQC (version 0.11.5)