##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727264.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7387323
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.554925647626344	31.0	31.0	34.0	30.0	34.0
2	31.77262399383376	31.0	31.0	34.0	30.0	34.0
3	31.948182988614413	31.0	31.0	34.0	30.0	34.0
4	35.657885407203665	37.0	35.0	37.0	35.0	37.0
5	35.45636098489263	37.0	35.0	37.0	33.0	37.0
6	35.46617969730036	37.0	35.0	37.0	33.0	37.0
7	35.36807284587394	37.0	35.0	37.0	33.0	37.0
8	35.32743430874757	37.0	35.0	37.0	33.0	37.0
9	36.875167905884176	39.0	37.0	39.0	33.0	39.0
10	36.66338888931755	39.0	35.0	39.0	32.0	39.0
11	36.73048017529489	39.0	35.0	39.0	32.0	39.0
12	36.564851570724606	38.0	35.0	39.0	32.0	39.0
13	36.55663993573856	38.0	35.0	39.0	32.0	39.0
14	37.55790074428856	39.0	36.0	41.0	32.0	41.0
15	37.48192883944563	39.0	36.0	41.0	32.0	41.0
16	37.41003486648682	39.0	36.0	41.0	32.0	41.0
17	37.72642308993393	39.0	37.0	41.0	32.0	41.0
18	37.75143066033528	39.0	37.0	41.0	32.0	41.0
19	37.847124188288504	40.0	37.0	41.0	32.0	41.0
20	37.813593638724065	40.0	37.0	41.0	32.0	41.0
21	37.770992821080114	40.0	37.0	41.0	32.0	41.0
22	37.712853627762044	40.0	37.0	41.0	32.0	41.0
23	37.604355867477295	40.0	37.0	41.0	32.0	41.0
24	37.499248374546504	39.0	37.0	41.0	32.0	41.0
25	37.42292695202308	39.0	36.0	41.0	31.0	41.0
26	37.148711515660004	39.0	36.0	41.0	31.0	41.0
27	36.969493143862806	39.0	36.0	41.0	30.0	41.0
28	36.79508544570205	39.0	36.0	41.0	30.0	41.0
29	36.63803884032146	39.0	36.0	40.0	30.0	41.0
30	36.49880044503266	39.0	36.0	40.0	30.0	41.0
31	36.34378556345783	39.0	35.0	40.0	29.0	41.0
32	36.212726315067044	39.0	35.0	40.0	29.0	41.0
33	36.044168232524825	39.0	35.0	40.0	28.0	41.0
34	35.92060182017221	38.0	35.0	40.0	27.0	41.0
35	35.76837793609403	38.0	35.0	40.0	27.0	41.0
36	35.686016436535944	38.0	35.0	40.0	26.0	41.0
37	35.61594639898648	38.0	35.0	40.0	26.0	41.0
38	35.516063396713534	38.0	34.0	40.0	26.0	41.0
39	35.38178403732989	38.0	34.0	40.0	25.0	41.0
40	35.2973106766822	38.0	34.0	40.0	25.0	41.0
41	35.10157860973454	38.0	34.0	40.0	24.0	41.0
42	34.93115354506632	38.0	34.0	40.0	24.0	41.0
43	34.64303537289489	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	10.0
11	21.0
12	44.0
13	41.0
14	75.0
15	148.0
16	296.0
17	553.0
18	999.0
19	2144.0
20	4176.0
21	7906.0
22	14091.0
23	23069.0
24	34889.0
25	49821.0
26	68284.0
27	89972.0
28	118055.0
29	149920.0
30	189050.0
31	236206.0
32	292439.0
33	358185.0
34	445537.0
35	555689.0
36	712635.0
37	947402.0
38	1464685.0
39	1620977.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.93274695041763	21.005119175105786	12.765273699281865	26.29686017519472
2	19.022939703597636	22.660536164453617	34.22456280847609	24.091961323472656
3	18.738357589075232	25.09241845794478	30.226159056535096	25.943064896444895
4	14.636750010795522	16.57808924829739	34.82518633610579	33.95997440480131
5	13.1428935759273	38.68854793542938	34.687951237545725	13.48060725109759
6	34.15885565041626	37.8511810029154	14.728718373353919	13.26124497331442
7	28.566721666292377	31.808816265377864	21.35274171712811	18.271720351201644
8	26.70315078953499	36.320951446146324	19.082826079217057	17.893071685101628
9	27.37597909283241	14.339849496224815	18.28803207873813	39.99613933220464
10	15.73296578476398	27.795481529642064	34.034886521138986	22.436666164454973
11	36.86497801707059	22.359209147887537	21.016192198445903	19.75962063659596
12	21.132377723297058	25.60045093466199	30.232737894363087	23.034433447677866
13	30.113669051698427	20.639370987298104	24.616021256956007	24.630938704047463
14	22.282699700554584	20.76906614209234	25.00299499561614	31.94523916173694
15	24.67186015827384	28.810016835597956	21.733935283457892	24.78418772267031
16	24.604772256472337	27.03707418776734	23.82637120374999	24.531782352010328
17	23.709671284171545	27.09464578711395	24.93689527315917	24.258787655555334
18	24.05439697167702	26.348259037813833	25.596349313547005	24.000994676962144
19	24.724355493864287	26.138940993916197	25.6056490287483	23.531054483471213
20	24.486678056448866	25.68316019212914	25.477713645389542	24.35244810603246
21	24.65725405535943	25.996237067202827	25.542067133114394	23.80444174432335
22	24.857719095266308	25.489355210270354	25.272050511396348	24.38087518306699
23	24.30498842408813	25.65642520301332	25.396168544410475	24.64241782848807
24	24.337923223338144	25.790641616726383	25.35037387697817	24.521061282957305
25	24.715082852069685	25.412020024033062	25.30055880865098	24.572338315246267
26	24.544303802608873	25.806168215468578	25.39033422526672	24.25919375665583
27	24.83501804374873	25.92993429419561	24.955440556748364	24.279607105307292
28	24.512966334354136	25.947532008550322	25.190925048221118	24.34857660887442
29	24.510069479837284	26.035128015926745	25.35718283876311	24.097619665472862
30	24.721363882423987	25.957129531225316	25.11149167296462	24.21001491338608
31	24.687143096355744	25.501240435811457	25.432839473785023	24.378776994047776
32	24.313787281265487	25.888038197328044	25.155621325884898	24.64255319552157
33	24.283817020049074	25.52453710227643	25.36332850208391	24.82831737559059
34	24.682797814580464	25.549742443913715	25.707837602335786	24.059622139170035
35	24.70436178301666	25.50795464067295	25.516645204223504	24.271038372086885
36	24.390337338708488	25.575164372804597	25.66890604350182	24.36559224498509
37	24.46435603262508	25.227284633418627	25.87649138937068	24.43186794458561
38	24.336461259376367	25.052133228775837	25.95254058878974	24.658864923058054
39	24.242895565822696	25.17584516068947	26.078973398076677	24.50228587541116
40	24.448084915198645	24.875885351161713	26.29040316769688	24.385626565942765
41	23.824746799348016	25.082712641642992	26.55199995993136	24.540540599077637
42	24.003905068182345	25.116838670787782	26.461317584191193	24.417938676838688
43	23.722734202904082	24.605773972520222	26.551566785424164	25.119925039151532
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	678.0
1	743.5
2	809.0
3	918.5
4	1028.0
5	1028.0
6	1425.5
7	1823.0
8	2283.0
9	2743.0
10	4004.0
11	5265.0
12	5265.0
13	9332.0
14	13399.0
15	19743.0
16	26087.0
17	26677.5
18	27268.0
19	27268.0
20	33912.0
21	40556.0
22	43970.0
23	47384.0
24	59084.5
25	70785.0
26	70785.0
27	87479.5
28	104174.0
29	121153.5
30	138133.0
31	161521.0
32	184909.0
33	184909.0
34	215422.0
35	245935.0
36	284021.0
37	322107.0
38	358646.0
39	395185.0
40	395185.0
41	428472.5
42	461760.0
43	493472.5
44	525185.0
45	558010.0
46	590835.0
47	590835.0
48	619332.5
49	647830.0
50	650927.5
51	654025.0
52	636929.0
53	619833.0
54	619833.0
55	591592.0
56	563351.0
57	523151.0
58	482951.0
59	438063.5
60	393176.0
61	393176.0
62	338498.0
63	283820.0
64	240459.5
65	197099.0
66	163882.5
67	130666.0
68	130666.0
69	105463.5
70	80261.0
71	66022.0
72	51783.0
73	41524.0
74	31265.0
75	31265.0
76	24523.0
77	17781.0
78	14595.0
79	11409.0
80	8897.0
81	6385.0
82	6385.0
83	5117.0
84	3849.0
85	3096.5
86	2344.0
87	2005.5
88	1667.0
89	1667.0
90	1218.5
91	770.0
92	594.5
93	419.0
94	417.5
95	416.0
96	416.0
97	244.0
98	72.0
99	87.5
100	103.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	7387323.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.84621728883767
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.58993305005251	44.2005297935606
2	9.80998211299059	10.760808211373856
3	3.5419847520579215	5.827933960353557
4	1.722368925959099	3.7786168145877883
5	0.9899420945874923	2.7147289611556347
6	0.6384920316215167	2.101132362210307
7	0.46300774344544005	1.7775956312396104
8	0.3193825285892922	1.4013538849013385
9	0.2521042747254149	1.2444269247931437
>10	1.5133310460776614	15.514840076207603
>50	0.10980439313795214	4.124503406210005
>100	0.04607210247653622	4.412610110795356
>500	0.0026777051247601964	0.9800360009149477
>1k	8.42868412979401E-4	0.7640288486193382
>5k	4.9580493917143184E-5	0.24383985900099733
>10k+	2.4790246958571592E-5	0.15301515407590188
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11254	0.1523420594984137	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9675	0.13096760490911252	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8259	0.11179963296582539	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.082936267982326E-4
2	0.0	0.0	0.0	0.0	1.082936267982326E-4
3	0.0	0.0	0.0	0.0	1.082936267982326E-4
4	0.0	0.0	0.0	0.0	1.2183033014801166E-4
5	0.0	0.0	0.0	0.0	1.2183033014801166E-4
6	2.707340669955815E-5	0.0	0.0	0.0	1.3536703349779073E-4
7	2.707340669955815E-5	0.0	0.0	0.0	1.489037368475698E-4
8	2.707340669955815E-5	0.0	0.0	0.0	1.489037368475698E-4
9	2.707340669955815E-5	0.0	0.0	2.707340669955815E-5	2.3012395694624427E-4
10	2.707340669955815E-5	0.0	0.0	2.707340669955815E-5	2.3012395694624427E-4
11	2.707340669955815E-5	0.0	0.0	4.061011004933722E-5	2.3012395694624427E-4
12	2.707340669955815E-5	0.0	0.0	4.061011004933722E-5	2.3012395694624427E-4
13	2.707340669955815E-5	0.0	0.0	4.061011004933722E-5	2.3012395694624427E-4
14	2.707340669955815E-5	0.0	0.0	4.061011004933722E-5	3.248808803946978E-4
15	2.707340669955815E-5	0.0	0.0	8.122022009867445E-5	3.248808803946978E-4
16	4.061011004933722E-5	0.0	0.0	1.082936267982326E-4	4.196378038431513E-4
17	4.061011004933722E-5	0.0	0.0	1.3536703349779073E-4	4.196378038431513E-4
18	4.061011004933722E-5	0.0	0.0	1.7597714354712797E-4	5.008580239418257E-4
19	4.061011004933722E-5	0.0	0.0	1.8951384689690703E-4	5.143947272916048E-4
20	4.061011004933722E-5	0.0	0.0	2.030505502466861E-4	5.143947272916048E-4
21	4.061011004933722E-5	0.0	0.0	2.7073406699558146E-4	5.414681339911629E-4
22	4.061011004933722E-5	0.0	0.0	4.061011004933722E-4	5.685415406907211E-4
23	4.061011004933722E-5	0.0	0.0	5.414681339911629E-4	7.174452775382909E-4
24	4.061011004933722E-5	0.0	0.0	8.392756076863026E-4	7.445186842378491E-4
25	4.061011004933722E-5	1.3536703349779074E-5	0.0	9.746426411840933E-4	7.715920909374072E-4
26	4.061011004933722E-5	1.3536703349779074E-5	0.0	0.0016920879187223843	7.986654976369654E-4
27	4.061011004933722E-5	1.3536703349779074E-5	0.0	0.008473976296961701	8.257389043365235E-4
28	4.061011004933722E-5	1.3536703349779074E-5	0.0	0.02016968799117082	8.257389043365235E-4
29	4.061011004933722E-5	1.3536703349779074E-5	0.0	0.03807874652292854	8.257389043365235E-4
30	4.061011004933722E-5	1.3536703349779074E-5	0.0	0.0645565382750964	8.257389043365235E-4
31	4.061011004933722E-5	1.3536703349779074E-5	0.0	0.17581470310693062	8.528123110360816E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3945	0.0	20.258556	1
TAGTACT	810	0.0	14.160494	4
TATACTG	1505	0.0	13.890365	5
TCTTATA	3385	0.0	13.007386	37
AAGACGG	1170	0.0	12.965813	5
TACCGTG	670	0.0	12.701492	7
GTATCAA	6310	0.0	12.577655	2
GTATTAG	1265	0.0	12.577075	1
ATACCGT	1165	0.0	12.386267	6
GTATAAG	1225	0.0	12.383674	1
ATTATAC	1095	0.0	12.333334	3
GTACATG	13245	0.0	12.333333	1
CTTATAC	2375	0.0	12.307369	37
GTACTAG	515	0.0	12.213592	1
TACATGG	13600	0.0	11.888969	2
GTTACGG	1215	0.0	11.876543	25
ACGGACC	1200	0.0	11.870833	8
TATACCG	440	0.0	11.772728	5
TTATACT	1395	0.0	11.670251	4
TTAGTAC	450	0.0	11.511111	3
>>END_MODULE