FastQCFastQC Report
Thu 9 Feb 2017
SRR2727263.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727263.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4110725
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG88270.2147309781121335No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC83190.20237306071313452No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG56600.1376886072408152No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA52160.12688759282121767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG7600.015.3355265
CAAGACG7950.014.6603774
TATACCG2650.013.9622645
TAGACCG2405.456968E-1213.8749995
TCGTTAC7500.013.56666623
GGACCGT3750.013.326
CGACGGT4250.013.0588247
GTATAAA6150.012.6341471
GTATTAG6350.012.5275591
ATACCGT6100.012.4344266
ACGGACC7600.012.4144748
CGCGCAA3150.012.33333421
GTATAAG5700.012.3333331
GACGGAC8600.012.2616277
ACCGTCG5200.012.0961538
TACGGAT13650.012.0622727
AGACGGA9250.012.06
TAGACAG7250.011.9931045
CGCGGTT3550.011.98591518
GTTACGG10750.011.87441925
Produced by FastQC (version 0.11.5)