##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727263.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4110725
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.5837600423283	31.0	31.0	34.0	30.0	34.0
2	31.79905320837565	31.0	31.0	34.0	30.0	34.0
3	31.97627279859392	31.0	31.0	34.0	30.0	34.0
4	35.67358799238577	37.0	35.0	37.0	35.0	37.0
5	35.469983032190186	37.0	35.0	37.0	33.0	37.0
6	35.48842649410992	37.0	35.0	37.0	33.0	37.0
7	35.391123706888685	37.0	35.0	37.0	33.0	37.0
8	35.353617914114906	37.0	35.0	37.0	33.0	37.0
9	36.90468226407751	39.0	37.0	39.0	33.0	39.0
10	36.690700302258115	39.0	35.0	39.0	32.0	39.0
11	36.768527936069674	39.0	35.0	39.0	32.0	39.0
12	36.59379890408626	38.0	35.0	39.0	32.0	39.0
13	36.59134070024144	38.0	35.0	39.0	32.0	39.0
14	37.59306497029113	39.0	36.0	41.0	32.0	41.0
15	37.52408078866866	39.0	36.0	41.0	32.0	41.0
16	37.42257412013696	39.0	36.0	41.0	32.0	41.0
17	37.754890439034476	39.0	37.0	41.0	32.0	41.0
18	37.78031539448637	40.0	37.0	41.0	32.0	41.0
19	37.858174166357514	40.0	37.0	41.0	32.0	41.0
20	37.83197100268201	40.0	37.0	41.0	32.0	41.0
21	37.780450163900525	40.0	37.0	41.0	32.0	41.0
22	37.74264296444058	40.0	37.0	41.0	32.0	41.0
23	37.62912795188197	40.0	37.0	41.0	32.0	41.0
24	37.52993085161377	39.0	37.0	41.0	32.0	41.0
25	37.44901982010472	39.0	37.0	41.0	32.0	41.0
26	37.182371722749636	39.0	36.0	41.0	31.0	41.0
27	37.01135420150947	39.0	36.0	41.0	30.0	41.0
28	36.82391889508542	39.0	36.0	41.0	30.0	41.0
29	36.674639631695136	39.0	36.0	40.0	30.0	41.0
30	36.53877016827932	39.0	36.0	40.0	30.0	41.0
31	36.39691198024679	39.0	35.0	40.0	30.0	41.0
32	36.24362758394201	39.0	35.0	40.0	29.0	41.0
33	36.097487426183946	39.0	35.0	40.0	28.0	41.0
34	35.97886552858681	38.0	35.0	40.0	27.0	41.0
35	35.8291914443316	38.0	35.0	40.0	27.0	41.0
36	35.76035662808872	38.0	35.0	40.0	27.0	41.0
37	35.695005625528346	38.0	35.0	40.0	26.0	41.0
38	35.60775629603051	38.0	35.0	40.0	26.0	41.0
39	35.47236241782167	38.0	34.0	40.0	25.0	41.0
40	35.398768830315824	38.0	34.0	40.0	25.0	41.0
41	35.20992404016323	38.0	34.0	40.0	24.0	41.0
42	35.050626835898775	38.0	34.0	40.0	24.0	41.0
43	34.770541449501	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	4.0
12	22.0
13	11.0
14	36.0
15	79.0
16	169.0
17	282.0
18	577.0
19	1147.0
20	2318.0
21	4351.0
22	7789.0
23	12620.0
24	18844.0
25	26957.0
26	36971.0
27	48996.0
28	63812.0
29	82378.0
30	103614.0
31	129794.0
32	159574.0
33	196878.0
34	244273.0
35	306323.0
36	394027.0
37	523692.0
38	830147.0
39	915035.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.910423343814045	19.855524268833356	12.181914382499437	27.05213800485316
2	19.14596573597115	22.35089917228713	33.574612750792134	24.928522340949588
3	19.23429565344313	24.529055093687855	29.7760370737522	26.460612179116822
4	15.108891983774154	16.14158086468932	33.850646783718204	34.89888036781833
5	13.399923371181483	38.570154899683146	34.41502411375122	13.614897615384148
6	35.24631299831538	36.534090701761855	14.58903234830839	13.630563951614375
7	29.062537630223378	31.40908233948999	20.868119370670623	18.660260659616004
8	27.41132038752288	35.77103795559178	19.07400762639194	17.743634030493403
9	28.18091699152826	13.756916359036422	17.32879236631008	40.73337428312524
10	15.954314628198189	27.40158974390162	33.750518460855446	22.893577167044743
11	37.74480170772795	21.695005138996162	20.201594609223434	20.358598544052448
12	21.68442306405804	24.950732535015113	29.694178034288356	23.67066636663849
13	30.922014973027874	19.85214287017497	23.884472933606602	25.341369223190558
14	22.793813743317784	20.196631980976594	24.160677252796038	32.84887702290958
15	25.786765108344632	27.734669675057322	21.137220320016542	25.341344896581504
16	25.179281027069432	26.378534200171504	23.640744637503115	24.801440135255948
17	24.787890214013345	26.308692505579916	24.052423842509533	24.85099343789721
18	25.110558356494295	25.54778050100651	24.879747489798028	24.461913652701167
19	25.73570355594208	25.85198474721612	24.503950033145006	23.90836166369679
20	25.159625126954495	24.892154060415134	24.593253014979112	25.354967797651266
21	24.988146859738855	25.503992604710845	25.045119778141327	24.462740757408973
22	25.565319986133833	24.797328938325965	23.87401249171375	25.763338583826457
23	24.60653534352213	25.001283228627553	24.696568123627827	25.69561330422249
24	24.749016292746415	25.56806889295684	24.42369654987867	25.259218264418077
25	25.526227125385425	24.587487608633513	23.927409398585407	25.958875867395655
26	25.29334849691964	25.045168431359432	24.54635131272464	25.115131758996284
27	26.008769742563658	25.03385655814972	23.734621021839214	25.222752677447406
28	24.984157295854136	25.514477373212756	24.12520905679655	25.37615627413656
29	25.383697522943034	25.324291943635245	24.461621733392526	24.83038880002919
30	25.57310450103084	25.37827268912418	24.23239209628472	24.816230713560262
31	26.009864439971054	24.684112899792616	24.10555315668161	25.20046950355472
32	24.909888158414876	25.235037615019245	24.2832833624239	25.57179086414197
33	25.062221384305687	24.73407975478778	24.47463646923596	25.72906239167057
34	25.47258695242323	24.92095676553406	24.709436899816943	24.897019382225764
35	25.406394249189617	25.094551447737324	24.76098498440056	24.738069318672498
36	25.415322114712126	24.935041872175834	24.61490369703641	25.03473231607563
37	25.13780415863382	24.92404624488381	25.091534948214733	24.846614648267643
38	25.76029775769481	24.349913944620475	24.80788668665503	25.081901611029682
39	25.25530168036052	24.397910344282334	24.878847405263063	25.467940570094083
40	25.65479325423131	24.209500757165706	25.270749077109265	24.864956911493717
41	24.80075899020246	24.73707192770132	25.06448375894763	25.397685323148593
42	25.16186317498738	24.697200615463206	25.37119364588972	24.769742563659694
43	24.94075862530332	23.88931392880818	25.137171666798437	26.03275577909006
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	104.0
1	106.5
2	109.0
3	131.0
4	153.0
5	153.0
6	244.0
7	335.0
8	398.5
9	462.0
10	665.0
11	868.0
12	868.0
13	1586.0
14	2304.0
15	3542.5
16	4781.0
17	5372.5
18	5964.0
19	5964.0
20	8018.0
21	10072.0
22	12372.0
23	14672.0
24	19043.5
25	23415.0
26	23415.0
27	30229.5
28	37044.0
29	45003.5
30	52963.0
31	64871.0
32	76779.0
33	76779.0
34	92799.5
35	108820.0
36	129691.0
37	150562.0
38	171469.5
39	192377.0
40	192377.0
41	214069.5
42	235762.0
43	257605.0
44	279448.0
45	308684.5
46	337921.0
47	337921.0
48	373708.5
49	409496.0
50	419969.5
51	430443.0
52	402504.0
53	374565.0
54	374565.0
55	356523.5
56	338482.0
57	315052.5
58	291623.0
59	264310.5
60	236998.0
61	236998.0
62	203957.5
63	170917.0
64	145408.0
65	119899.0
66	99221.0
67	78543.0
68	78543.0
69	63456.0
70	48369.0
71	39848.5
72	31328.0
73	25013.0
74	18698.0
75	18698.0
76	14548.0
77	10398.0
78	8578.0
79	6758.0
80	5219.0
81	3680.0
82	3680.0
83	2985.5
84	2291.0
85	1822.0
86	1353.0
87	1184.0
88	1015.0
89	1015.0
90	717.0
91	419.0
92	327.5
93	236.0
94	221.5
95	207.0
96	207.0
97	122.5
98	38.0
99	46.0
100	54.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4110725.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.65255551002766
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.21565766097	46.01074553921506
2	9.62499760085626	10.905614217327846
3	3.354004027654099	5.700386980725906
4	1.6916819433185053	3.8335242079665237
5	0.9463262893560916	2.680590131917224
6	0.6321991229674861	2.148941754438379
7	0.45721896854419575	1.8131836096981349
8	0.33574373809515334	1.521659260766389
9	0.231547913277871	1.1806002909185094
>10	1.3944108953355234	14.224246561015446
>50	0.073970917031801	2.8313023199011234
>100	0.037660950017784835	4.114296287200063
>500	0.002808473846873922	1.0891011417122418
>1k	0.001598669584014062	1.259884292441284
>5k	1.7282914421773175E-4	0.6859234047559537
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	8827	0.2147309781121335	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	8319	0.20237306071313452	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	5660	0.1376886072408152	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	5216	0.12688759282121767	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.432660905314756E-5
2	0.0	0.0	0.0	0.0	2.432660905314756E-5
3	0.0	0.0	0.0	0.0	2.432660905314756E-5
4	0.0	0.0	0.0	0.0	2.432660905314756E-5
5	0.0	0.0	0.0	0.0	2.432660905314756E-5
6	2.432660905314756E-5	0.0	0.0	0.0	7.297982715944267E-5
7	2.432660905314756E-5	0.0	0.0	0.0	7.297982715944267E-5
8	2.432660905314756E-5	0.0	0.0	0.0	9.730643621259024E-5
9	2.432660905314756E-5	0.0	0.0	4.865321810629512E-5	1.9461287242518048E-4
10	2.432660905314756E-5	0.0	0.0	1.4595965431888535E-4	1.9461287242518048E-4
11	2.432660905314756E-5	0.0	0.0	1.4595965431888535E-4	2.1893948147832804E-4
12	2.432660905314756E-5	0.0	0.0	1.4595965431888535E-4	2.1893948147832804E-4
13	2.432660905314756E-5	2.432660905314756E-5	0.0	1.4595965431888535E-4	2.4326609053147558E-4
14	2.432660905314756E-5	2.432660905314756E-5	0.0	1.4595965431888535E-4	4.135523539035085E-4
15	2.432660905314756E-5	2.432660905314756E-5	0.0	1.702862633720329E-4	4.378789629566561E-4
16	2.432660905314756E-5	2.432660905314756E-5	0.0	2.919193086377707E-4	4.8653218106295117E-4
17	2.432660905314756E-5	2.432660905314756E-5	0.0	3.405725267440658E-4	4.8653218106295117E-4
18	2.432660905314756E-5	2.432660905314756E-5	0.0	4.135523539035085E-4	5.595120082223939E-4
19	2.432660905314756E-5	2.432660905314756E-5	0.0	4.622055720098036E-4	5.595120082223939E-4
20	2.432660905314756E-5	2.432660905314756E-5	0.0	4.8653218106295117E-4	5.595120082223939E-4
21	2.432660905314756E-5	2.432660905314756E-5	0.0	5.838386172755414E-4	6.08165226328689E-4
22	2.432660905314756E-5	2.432660905314756E-5	0.0	6.324918353818365E-4	6.324918353818365E-4
23	2.432660905314756E-5	2.432660905314756E-5	0.0	7.297982715944268E-4	9.730643621259023E-4
24	2.432660905314756E-5	2.432660905314756E-5	0.0	9.730643621259023E-4	9.730643621259023E-4
25	2.432660905314756E-5	2.432660905314756E-5	0.0	0.001216330452657378	9.730643621259023E-4
26	2.432660905314756E-5	2.432660905314756E-5	0.0	0.0015082497612951486	9.730643621259023E-4
27	2.432660905314756E-5	2.432660905314756E-5	0.0	0.0036733179670252816	9.730643621259023E-4
28	2.432660905314756E-5	2.432660905314756E-5	0.0	0.008392680123335907	9.730643621259023E-4
29	2.432660905314756E-5	2.432660905314756E-5	0.0	0.015958255538864798	9.730643621259023E-4
30	2.432660905314756E-5	2.432660905314756E-5	0.0	0.028535112419342087	9.730643621259023E-4
31	2.432660905314756E-5	2.432660905314756E-5	0.0	0.09696586368584617	9.730643621259023E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	760	0.0	15.335526	5
CAAGACG	795	0.0	14.660377	4
TATACCG	265	0.0	13.962264	5
TAGACCG	240	5.456968E-12	13.874999	5
TCGTTAC	750	0.0	13.566666	23
GGACCGT	375	0.0	13.32	6
CGACGGT	425	0.0	13.058824	7
GTATAAA	615	0.0	12.634147	1
GTATTAG	635	0.0	12.527559	1
ATACCGT	610	0.0	12.434426	6
ACGGACC	760	0.0	12.414474	8
CGCGCAA	315	0.0	12.333334	21
GTATAAG	570	0.0	12.333333	1
GACGGAC	860	0.0	12.261627	7
ACCGTCG	520	0.0	12.096153	8
TACGGAT	1365	0.0	12.06227	27
AGACGGA	925	0.0	12.0	6
TAGACAG	725	0.0	11.993104	5
CGCGGTT	355	0.0	11.985915	18
GTTACGG	1075	0.0	11.874419	25
>>END_MODULE