FastQCFastQC Report
Thu 9 Feb 2017
SRR2727262.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727262.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5779435
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT58940.10198228719589372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32750.022.0870231
AAGACGG10600.016.405665
TATACTG10450.015.0478475
CGAACGT2600.014.2307694
TCGTTAC13700.014.17883223
GTATCAA51250.014.0780492
TATAGCG1356.577464E-613.7037045
TACTTAC10550.013.67772531
CAAGACG12500.013.6164
CTATACT7900.013.5822784
TACGGAT25700.013.24513627
CGTTACG12050.013.2033224
AATTAAC9450.013.1164032
TTAGAGT9250.013.04
AAATCGC8100.012.79012314
GGGTAAG43850.012.6567841
ACGGATG27050.012.65249528
CAAATCG8350.012.62874213
GTTACGG19300.012.55699525
CTTATAC13300.012.51879737
Produced by FastQC (version 0.11.5)