##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727262.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5779435
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.573197033966125	31.0	31.0	34.0	30.0	34.0
2	31.7926553374162	31.0	31.0	34.0	30.0	34.0
3	31.962872841376363	31.0	31.0	34.0	30.0	34.0
4	35.6698812254139	37.0	35.0	37.0	35.0	37.0
5	35.47336115035466	37.0	35.0	37.0	33.0	37.0
6	35.47869488972538	37.0	35.0	37.0	33.0	37.0
7	35.38690477529378	37.0	35.0	37.0	33.0	37.0
8	35.348640135238135	37.0	35.0	37.0	33.0	37.0
9	36.8800554033396	39.0	37.0	39.0	33.0	39.0
10	36.68012340306622	39.0	35.0	39.0	32.0	39.0
11	36.74633489259763	39.0	35.0	39.0	32.0	39.0
12	36.58085920163476	38.0	35.0	39.0	32.0	39.0
13	36.57685673426555	38.0	35.0	39.0	32.0	39.0
14	37.557207062628095	39.0	36.0	41.0	32.0	41.0
15	37.492212820111305	39.0	36.0	41.0	32.0	41.0
16	37.40725209298141	39.0	36.0	41.0	32.0	41.0
17	37.738123190242646	39.0	37.0	41.0	32.0	41.0
18	37.76669743668715	40.0	37.0	41.0	32.0	41.0
19	37.85806363424798	40.0	37.0	41.0	32.0	41.0
20	37.826129024723	40.0	37.0	41.0	32.0	41.0
21	37.77923689772443	40.0	37.0	41.0	32.0	41.0
22	37.72707539750858	40.0	37.0	41.0	32.0	41.0
23	37.60644959239095	40.0	37.0	41.0	32.0	41.0
24	37.49705550802111	39.0	37.0	41.0	32.0	41.0
25	37.411835586004514	39.0	36.0	41.0	31.0	41.0
26	37.142770703364604	39.0	36.0	41.0	31.0	41.0
27	36.96304794499808	39.0	36.0	41.0	30.0	41.0
28	36.769825424111524	39.0	36.0	41.0	30.0	41.0
29	36.60777463540986	39.0	36.0	40.0	30.0	41.0
30	36.46841810661423	39.0	36.0	40.0	30.0	41.0
31	36.3164449466081	39.0	35.0	40.0	29.0	41.0
32	36.17911543256391	39.0	35.0	40.0	29.0	41.0
33	36.01376933904439	39.0	35.0	40.0	27.0	41.0
34	35.898947561483084	38.0	35.0	40.0	27.0	41.0
35	35.73720822191097	38.0	35.0	40.0	27.0	41.0
36	35.639924490888816	38.0	35.0	40.0	26.0	41.0
37	35.58035378890843	38.0	35.0	40.0	26.0	41.0
38	35.472037491554104	38.0	34.0	40.0	25.0	41.0
39	35.332018268221724	38.0	34.0	40.0	25.0	41.0
40	35.25044835697607	38.0	34.0	40.0	25.0	41.0
41	35.04903593517359	38.0	34.0	40.0	24.0	41.0
42	34.888143218151946	38.0	33.0	40.0	24.0	41.0
43	34.61187347898194	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	6.0
11	17.0
12	28.0
13	45.0
14	65.0
15	130.0
16	227.0
17	445.0
18	847.0
19	1709.0
20	3550.0
21	6513.0
22	11387.0
23	18755.0
24	28287.0
25	39632.0
26	54037.0
27	71775.0
28	93227.0
29	117807.0
30	147845.0
31	184294.0
32	227077.0
33	279781.0
34	346538.0
35	435137.0
36	555559.0
37	733851.0
38	1141504.0
39	1279359.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.22002150729267	20.80127209666689	12.762164467633946	26.216541928406496
2	19.212535481409514	22.70097336504347	33.98927403803313	24.097217115513885
3	18.865494637451587	25.090411086896903	30.341183870049584	25.70291040560193
4	14.763623087723973	16.409579829170152	34.504151357355866	34.32264572575001
5	13.119881095643432	39.34097364188714	34.13935791301399	13.399787349455439
6	34.28784993688829	37.406909152884324	14.834996846577564	13.470244063649819
7	28.630065049611254	32.33201861427631	20.852453570288446	18.185462765823996
8	27.17779506128194	35.983655841790764	19.13688794838942	17.701661148537877
9	28.147509228843305	14.11250407695562	17.919104549147104	39.82088214505397
10	15.981856357931182	27.88753571932204	33.475106130616574	22.6555017921302
11	37.25834791809234	22.206201817305672	20.573671993888677	19.961778270713314
12	21.309747406104577	25.619165195213718	29.670858829626077	23.400228569055624
13	30.365078939377295	20.477070855542106	24.245709139388193	24.91214106569241
14	22.654238692882608	20.75284175702296	24.534682023415783	32.058237526678646
15	25.168134947447285	28.615790297840533	21.493467787076074	24.72260696763611
16	24.873348346334893	26.84047835125752	23.691589229743045	24.594584072664542
17	23.990874540504393	26.80855481547937	24.33810571448593	24.862464929530308
18	24.257301968098957	26.266045037274406	25.174709984626524	24.301943010000112
19	25.26634523962982	25.894122868411877	25.089840096826073	23.749691795132225
20	24.686703804091575	25.139671957552945	25.046046888666453	25.12757734968903
21	24.92852675045225	25.780824596175922	25.39253404528297	23.898114608088854
22	25.355263966114332	25.0634361317326	24.65542046930193	24.925879432851136
23	24.524058147552484	25.41452581437459	25.162078992150615	24.89933704592231
24	24.417248398848677	25.379210943630305	25.094096568263165	25.10944408925786
25	25.016424615901034	24.95423860636896	24.80988193482581	25.21945484290419
26	24.74504860769262	25.47219581152829	25.116399786484315	24.66635579429477
27	25.512788014745386	25.58589204654088	24.456196842770964	24.44512309594277
28	24.652496308030113	26.02678289486775	24.436298703939052	24.884422093163085
29	24.811992867814933	25.980307763648174	24.98365324638135	24.224046122155542
30	25.476798337553756	25.562896719142962	24.569339390442146	24.390965552861136
31	25.151835776334536	25.26805820984231	25.01908923623157	24.561016777591583
32	24.803497227670178	25.629806373806435	24.508745232016622	25.057951166506758
33	24.599515350548973	25.005437382719936	24.893938594343563	25.501108672387524
34	25.20331139635622	25.365247640989132	25.3453321994278	24.086108763226854
35	25.16562605168152	25.088698116684416	25.19611346091789	24.549562370716167
36	24.69011244178713	25.302473338656807	25.406722283406598	24.60069193614947
37	24.78740568931046	25.231601358956368	25.65494723965232	24.32604571208085
38	25.127040965077036	24.53636038816943	25.447712449400328	24.8888861973532
39	24.593494000711143	25.070114985288356	25.670658117964816	24.665732896035685
40	25.208277279699487	24.478378941886188	25.76262212482708	24.550721653587246
41	24.11813611538152	25.18562800688995	26.014446048791967	24.681789828936566
42	24.458913371289754	24.9896918989486	26.02932639609235	24.522068333669296
43	24.029303902544108	24.41757715070764	25.880799766759203	25.672319179989046
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	475.0
1	458.0
2	441.0
3	466.0
4	491.0
5	491.0
6	674.0
7	857.0
8	1091.0
9	1325.0
10	1925.0
11	2525.0
12	2525.0
13	4856.0
14	7187.0
15	10672.0
16	14157.0
17	15152.0
18	16147.0
19	16147.0
20	20968.0
21	25789.0
22	29375.5
23	32962.0
24	41411.5
25	49861.0
26	49861.0
27	61906.0
28	73951.0
29	86822.0
30	99693.0
31	117489.5
32	135286.0
33	135286.0
34	156354.0
35	177422.0
36	204548.5
37	231675.0
38	259232.5
39	286790.0
40	286790.0
41	314443.0
42	342096.0
43	366485.5
44	390875.0
45	426123.0
46	461371.0
47	461371.0
48	496974.0
49	532577.0
50	538493.0
51	544409.0
52	529775.0
53	515141.0
54	515141.0
55	487393.5
56	459646.0
57	427902.5
58	396159.0
59	358626.5
60	321094.0
61	321094.0
62	275380.0
63	229666.0
64	196524.0
65	163382.0
66	135306.0
67	107230.0
68	107230.0
69	85087.5
70	62945.0
71	51500.0
72	40055.0
73	31657.0
74	23259.0
75	23259.0
76	18083.5
77	12908.0
78	10478.5
79	8049.0
80	6272.5
81	4496.0
82	4496.0
83	3669.0
84	2842.0
85	2273.0
86	1704.0
87	1433.0
88	1162.0
89	1162.0
90	865.0
91	568.0
92	450.0
93	332.0
94	307.5
95	283.0
96	283.0
97	169.0
98	55.0
99	76.0
100	97.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5779435.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.09226054873976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.3447357985863	42.65683646738202
2	9.84883659019397	10.457939966970798
3	3.502886718764411	5.579285230360806
4	1.7251160995233563	3.6636125373087878
5	1.0274047016598915	2.72736190547636
6	0.65483933310991	2.0860140294620595
7	0.47252153337329583	1.7561065455321534
8	0.3514324482505233	1.492667448623863
9	0.25433886242455356	1.2153082636363028
>10	1.6460682177203665	16.066423183043486
>50	0.11711230059598106	4.2277048997180735
>100	0.048906617518147094	4.798523604361371
>500	0.004194920799002871	1.5428930386620445
>1k	0.0015403122771356953	1.5384480053683889
>5k	6.554520327340371E-5	0.19087487409365453
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5894	0.10198228719589372	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.730272941905221E-5
2	0.0	0.0	0.0	0.0	1.730272941905221E-5
3	0.0	0.0	0.0	0.0	1.730272941905221E-5
4	0.0	0.0	0.0	1.730272941905221E-5	1.730272941905221E-5
5	0.0	0.0	0.0	1.730272941905221E-5	1.730272941905221E-5
6	0.0	0.0	0.0	1.730272941905221E-5	3.460545883810442E-5
7	0.0	0.0	0.0	1.730272941905221E-5	3.460545883810442E-5
8	0.0	0.0	0.0	1.730272941905221E-5	3.460545883810442E-5
9	0.0	0.0	0.0	1.730272941905221E-5	1.0381637651431325E-4
10	0.0	0.0	0.0	3.460545883810442E-5	1.0381637651431325E-4
11	0.0	0.0	0.0	3.460545883810442E-5	1.2111910593336546E-4
12	0.0	0.0	0.0	5.190818825715663E-5	1.2111910593336546E-4
13	0.0	0.0	0.0	6.921091767620884E-5	1.3842183535241767E-4
14	0.0	0.0	0.0	6.921091767620884E-5	1.9033002360957429E-4
15	0.0	0.0	0.0	6.921091767620884E-5	1.9033002360957429E-4
16	0.0	0.0	0.0	1.2111910593336546E-4	3.633573178000964E-4
17	0.0	0.0	0.0	1.5572456477146987E-4	3.633573178000964E-4
18	0.0	0.0	0.0	1.9033002360957429E-4	3.9796277663820077E-4
19	0.0	0.0	0.0	2.076327530286265E-4	4.325682354763052E-4
20	0.0	1.730272941905221E-5	0.0	2.4223821186673093E-4	4.325682354763052E-4
21	0.0	1.730272941905221E-5	0.0	3.9796277663820077E-4	4.325682354763052E-4
22	0.0	1.730272941905221E-5	0.0	5.190818825715663E-4	4.325682354763052E-4
23	0.0	1.730272941905221E-5	0.0	7.44017365019245E-4	5.709900708287228E-4
24	0.0	1.730272941905221E-5	0.0	0.0011073746828193414	6.055955296668273E-4
25	0.0	1.730272941905221E-5	0.0	0.0012284937887527068	6.055955296668273E-4
26	0.0	1.730272941905221E-5	0.0	0.0015572456477146988	6.055955296668273E-4
27	0.0	1.730272941905221E-5	0.0	0.004464104190115469	6.055955296668273E-4
28	0.0	1.730272941905221E-5	0.0	0.01010479398072649	6.055955296668273E-4
29	0.0	1.730272941905221E-5	0.0	0.018756158690252593	6.228982590858795E-4
30	0.0	1.730272941905221E-5	0.0	0.034640064296942524	6.228982590858795E-4
31	0.0	1.730272941905221E-5	0.0	0.11687993722569767	6.228982590858795E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3275	0.0	22.087023	1
AAGACGG	1060	0.0	16.40566	5
TATACTG	1045	0.0	15.047847	5
CGAACGT	260	0.0	14.230769	4
TCGTTAC	1370	0.0	14.178832	23
GTATCAA	5125	0.0	14.078049	2
TATAGCG	135	6.577464E-6	13.703704	5
TACTTAC	1055	0.0	13.677725	31
CAAGACG	1250	0.0	13.616	4
CTATACT	790	0.0	13.582278	4
TACGGAT	2570	0.0	13.245136	27
CGTTACG	1205	0.0	13.20332	24
AATTAAC	945	0.0	13.116403	2
TTAGAGT	925	0.0	13.0	4
AAATCGC	810	0.0	12.790123	14
GGGTAAG	4385	0.0	12.656784	1
ACGGATG	2705	0.0	12.652495	28
CAAATCG	835	0.0	12.628742	13
GTTACGG	1930	0.0	12.556995	25
CTTATAC	1330	0.0	12.518797	37
>>END_MODULE