FastQCFastQC Report
Thu 9 Feb 2017
SRR2727261.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727261.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2086312
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC130480.6254098140642435No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG106480.5103742872590484No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG89780.4303287331904337No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT87220.41805827699787956No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT86720.41566170352277126No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC83300.399269140953031No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG79560.38134277135922146No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC68810.3298164416443945No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG60050.2878284743604983No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA51870.2486205323077277No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC47930.22973553332387486No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA45610.2186154323993727No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT44630.21391814838816053No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG41200.1974776543489181No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCA40710.19512901234331204No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT39810.1908151800881172No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG38970.18678893664993537No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT38770.1858303072598921No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG38450.1842965002358228No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT34370.1647404606789397No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG33230.15927627315569293No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA32660.15654417939406953No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT31850.1526617303643942No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA30640.1468620225546323No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC30470.14604718757309548No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT29340.1406309315193509No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG28860.138330220983247No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27540.13200326700896128No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAG23360.11196791275705648No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC23050.11048203720248936No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG22560.10813339519688331No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCC21900.10496991820974046No Hit
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC21180.1015188524055846No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC21030.10079988036305212No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT358.871829E-426.42857234
CTAGTAC1950.020.8717963
TGCGACG1251.8189894E-1120.72000122
AGGTTAT2850.020.1228071
TAGTACT1950.019.9230774
AGACCGC851.2463042E-619.58823617
ATTAGAG2400.019.2708343
CGGGTAC609.2402566E-418.524
GGTTATC3300.018.52
ACGTAAT500.00703675918.49999821
AAGACGG6450.018.0697675
TAGGTCG1603.45608E-1117.3437521
GTACTAG1603.45608E-1117.343751
CGTCATT1603.45608E-1117.3437526
ATGCGAC1502.5102054E-1017.26666621
CGAGCCG6350.016.60629815
GTATAAG2800.016.5178591
GATTTCG1352.2220775E-816.44444519
TTATCTA3600.016.4444454
GTCGTCA1801.0913936E-1116.44444524
Produced by FastQC (version 0.11.5)