FastQCFastQC Report
Thu 9 Feb 2017
SRR2727260.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727260.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5365235
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33850.019.8389971
TTCGCGA904.4508502E-516.44444522
TACTTAC16650.015.33333331
TACGGAT31350.014.92982527
CGTTACG16100.014.82298224
TCGTTAC17500.014.69428623
GTTACGG28200.014.367022525
ACGGATG32800.014.21341428
TATACTG9900.014.015155
GTATCAA49100.013.6395112
ATTATAC7500.013.5666683
GTACTTA19400.013.54123730
TTACGGA35050.013.45934426
TAATCCC14900.013.4093955
CGGATGG35050.013.30099929
TCTTATA21700.013.12903237
TTATACT9600.013.1041664
TAATACG1703.7388054E-713.0588244
CGAACGT2551.6370905E-1113.0588244
GGGCGTC20100.012.97761211
Produced by FastQC (version 0.11.5)