##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727260.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5365235
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.54815157211194	31.0	31.0	34.0	30.0	34.0
2	31.76593420418677	31.0	31.0	34.0	30.0	34.0
3	31.937888275164088	31.0	31.0	34.0	30.0	34.0
4	35.64309671430981	37.0	35.0	37.0	33.0	37.0
5	35.4406002719359	37.0	35.0	37.0	33.0	37.0
6	35.44661491994293	37.0	35.0	37.0	33.0	37.0
7	35.3539237330704	37.0	35.0	37.0	33.0	37.0
8	35.31750538420032	37.0	35.0	37.0	33.0	37.0
9	36.842805021588056	39.0	37.0	39.0	33.0	39.0
10	36.63559359468877	39.0	35.0	39.0	32.0	39.0
11	36.71094816909231	39.0	35.0	39.0	32.0	39.0
12	36.53700443689792	38.0	35.0	39.0	32.0	39.0
13	36.53840456196234	38.0	35.0	39.0	32.0	39.0
14	37.50241638996241	39.0	36.0	41.0	32.0	41.0
15	37.43794130173236	39.0	36.0	41.0	32.0	41.0
16	37.33842245493441	39.0	36.0	41.0	32.0	41.0
17	37.68183947208277	39.0	37.0	41.0	32.0	41.0
18	37.707637037333875	39.0	37.0	41.0	32.0	41.0
19	37.807681676571484	40.0	37.0	41.0	32.0	41.0
20	37.76519835571042	40.0	37.0	41.0	32.0	41.0
21	37.72485361778189	40.0	37.0	41.0	32.0	41.0
22	37.67080715010619	40.0	37.0	41.0	32.0	41.0
23	37.55558051045294	39.0	37.0	41.0	32.0	41.0
24	37.451432602672575	39.0	36.0	41.0	32.0	41.0
25	37.35486777373219	39.0	36.0	41.0	31.0	41.0
26	37.086526498839284	39.0	36.0	41.0	31.0	41.0
27	36.90379936759527	39.0	36.0	41.0	30.0	41.0
28	36.70383701739066	39.0	36.0	40.0	30.0	41.0
29	36.54894650467314	39.0	36.0	40.0	30.0	41.0
30	36.416666185171756	39.0	35.0	40.0	30.0	41.0
31	36.24995941463887	39.0	35.0	40.0	29.0	41.0
32	36.10476148761424	39.0	35.0	40.0	28.0	41.0
33	35.940520778679776	38.0	35.0	40.0	27.0	41.0
34	35.81199854246832	38.0	35.0	40.0	27.0	41.0
35	35.64775559691235	38.0	35.0	40.0	26.0	41.0
36	35.53299100598576	38.0	34.0	40.0	26.0	41.0
37	35.48358273216364	38.0	34.0	40.0	25.0	41.0
38	35.37631902423659	38.0	34.0	40.0	25.0	41.0
39	35.21156575620639	38.0	34.0	40.0	25.0	41.0
40	35.14946801025491	38.0	34.0	40.0	24.0	41.0
41	34.94258070708925	38.0	34.0	40.0	24.0	41.0
42	34.78542673340497	38.0	33.0	40.0	24.0	41.0
43	34.514505701986955	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	6.0
11	10.0
12	33.0
13	27.0
14	49.0
15	109.0
16	205.0
17	399.0
18	733.0
19	1683.0
20	3309.0
21	6278.0
22	10795.0
23	17809.0
24	26702.0
25	38132.0
26	51672.0
27	67937.0
28	88484.0
29	112893.0
30	141344.0
31	175813.0
32	216080.0
33	264247.0
34	328065.0
35	407580.0
36	520535.0
37	681929.0
38	1054584.0
39	1147792.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.21525618169567	20.3821081462415	12.796475830042858	26.606159842019967
2	19.402710226113115	22.318686879512267	33.99427984049161	24.284323053883007
3	19.074784235918838	24.55564388139569	30.31824328291305	26.05132859977242
4	15.146307664063178	16.21662424851847	34.01213553553572	34.62493255188263
5	13.397866076695616	39.01776902596065	33.93374195165729	13.650622945686441
6	34.869302090215996	36.78750325009063	14.694398288239006	13.648796371454372
7	28.84356416820512	32.13831267409535	20.563628620181596	18.45449453751793
8	27.219814229945193	36.191518172083796	18.98722050385491	17.6014470941161
9	28.564750658638438	13.96026455504745	17.541710661322384	39.93327412499173
10	16.058718770007278	27.753323013810206	33.28562495398617	22.902333262196343
11	37.56981753828118	21.964368755515835	20.239001646712584	20.226812059490403
12	21.533707284023905	25.064922598916915	29.46506536992322	23.93630474713596
13	30.724469664422898	20.280751914874184	23.82972973224845	25.165048688454466
14	22.67719866883743	20.429654991813035	24.385455623099453	32.50769071625008
15	25.686423800635016	28.30381148262844	21.22648868129728	24.78327603543927
16	24.93012887599518	26.736200744235806	23.588976065354082	24.744694314414932
17	24.355112124632004	26.273481031119793	24.0793926081523	25.2920142360959
18	24.331627598791105	26.372153316676716	24.997432544893186	24.298786539638993
19	25.799895065174216	25.644654893960844	24.833860958560063	23.721589082304874
20	24.79032139319154	24.949960253371938	24.804915348535523	25.454803004900995
21	24.992884747825585	25.542404013990065	25.50866830623449	23.95604293194986
22	25.650749687571935	24.67206003092129	24.254389602692147	25.422800678814628
23	24.816117094591384	25.237328840209234	25.078976037396313	24.86757802780307
24	24.577898265406827	24.960956975789504	25.03370309035858	25.427441668445088
25	25.33190065300029	24.573667323052952	24.557955056954633	25.536476966992126
26	24.918591636713025	25.10760479270712	24.983658684102377	24.99014488647748
27	25.866341362493905	25.329570838928774	24.14744181755319	24.65664598102413
28	24.642629819569876	26.211023375490544	23.882924047129343	25.263422757810233
29	25.062797808483694	25.87666709845887	24.777088049265316	24.283447043792115
30	26.200362146299277	25.10113722884459	24.06750123713127	24.630999387724863
31	25.47804523007846	24.997413906380615	24.854419237927136	24.67012162561379
32	25.03806450230046	25.483506314262094	24.293176347354777	25.185252836082668
33	24.886682503189515	24.606508382205067	24.576034414149614	25.930774700455807
34	25.403267517639023	25.17110620504041	25.239397715104744	24.18622856221582
35	25.48333856764895	24.86340300098691	24.813861834570154	24.83939659679399
36	25.076273453073348	24.97448853591688	25.195373548409343	24.75386446260043
37	24.915889052390064	25.135077960238462	25.55546961130314	24.393563376068336
38	25.697215499414284	24.175008177647392	24.960584205538062	25.167192117400262
39	24.56470219850575	24.94656804408381	25.73986041617935	24.748869341231092
40	25.722936646763845	23.956434340713873	25.58353921123679	24.737089801285496
41	24.396135490803292	24.84776528893888	25.68072041578794	25.07537880446989
42	24.64583564373229	24.820198928844682	25.75574042889081	24.778224998532217
43	24.179555974714994	24.22421385083785	25.535955088640105	26.06027508580705
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	370.0
1	395.0
2	420.0
3	450.0
4	480.0
5	480.0
6	660.5
7	841.0
8	1026.0
9	1211.0
10	1763.5
11	2316.0
12	2316.0
13	4312.5
14	6309.0
15	9367.5
16	12426.0
17	12857.5
18	13289.0
19	13289.0
20	17118.5
21	20948.0
22	23514.5
23	26081.0
24	33228.0
25	40375.0
26	40375.0
27	50033.5
28	59692.0
29	70426.5
30	81161.0
31	96541.0
32	111921.0
33	111921.0
34	131439.0
35	150957.0
36	176229.5
37	201502.0
38	227649.5
39	253797.0
40	253797.0
41	279447.0
42	305097.0
43	330524.0
44	355951.0
45	391051.5
46	426152.0
47	426152.0
48	464140.0
49	502128.0
50	511208.5
51	520289.0
52	510013.0
53	499737.0
54	499737.0
55	472464.5
56	445192.0
57	415144.5
58	385097.0
59	347267.0
60	309437.0
61	309437.0
62	263839.5
63	218242.0
64	186555.5
65	154869.0
66	127660.0
67	100451.0
68	100451.0
69	80826.0
70	61201.0
71	50434.5
72	39668.0
73	31633.5
74	23599.0
75	23599.0
76	18571.5
77	13544.0
78	11013.5
79	8483.0
80	6704.0
81	4925.0
82	4925.0
83	3939.5
84	2954.0
85	2338.0
86	1722.0
87	1443.0
88	1164.0
89	1164.0
90	865.0
91	566.0
92	429.5
93	293.0
94	263.0
95	233.0
96	233.0
97	145.5
98	58.0
99	72.5
100	87.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5365235.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.12893968134512
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.86928642253007	44.58238013261082
2	9.69830351013717	10.693143784434596
3	3.433183973564941	5.678033765808673
4	1.6735628387076893	3.6904697915222764
5	0.9652397807186771	2.660632282463737
6	0.666896444340257	2.2059176312242537
7	0.4595144131392331	1.7732779655263038
8	0.3307637925141297	1.4587725733027528
9	0.2526988659688331	1.2537918485586104
>10	1.5071939900409193	15.116517460525678
>50	0.09540181227011633	3.5954907473441904
>100	0.0430352722368277	4.571058439203072
>500	0.0033244928543660897	1.2051865510018749
>1k	0.0015604677645668465	1.41557318690057
>5k	3.3923212272131364E-5	0.09975383957270348
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.591553771642808E-5
2	0.0	0.0	0.0	1.8638512572142693E-5	5.591553771642808E-5
3	0.0	0.0	0.0	1.8638512572142693E-5	5.591553771642808E-5
4	0.0	0.0	0.0	1.8638512572142693E-5	5.591553771642808E-5
5	0.0	0.0	0.0	1.8638512572142693E-5	5.591553771642808E-5
6	0.0	0.0	0.0	1.8638512572142693E-5	1.8638512572142693E-4
7	0.0	0.0	0.0	1.8638512572142693E-5	1.8638512572142693E-4
8	0.0	0.0	0.0	1.8638512572142693E-5	2.42300663437855E-4
9	0.0	0.0	0.0	1.8638512572142693E-5	3.1685471372642577E-4
10	0.0	0.0	0.0	1.8638512572142693E-5	3.1685471372642577E-4
11	0.0	0.0	0.0	1.8638512572142693E-5	3.1685471372642577E-4
12	0.0	0.0	0.0	1.8638512572142693E-5	3.1685471372642577E-4
13	0.0	0.0	0.0	1.8638512572142693E-5	3.541317388707112E-4
14	0.0	0.0	0.0	3.7277025144285385E-5	4.4732430173142465E-4
15	0.0	0.0	0.0	5.591553771642808E-5	5.032398394478528E-4
16	1.8638512572142693E-5	0.0	0.0	9.319256286071347E-5	5.964324023085662E-4
17	1.8638512572142693E-5	0.0	0.0	1.1183107543285616E-4	5.964324023085662E-4
18	1.8638512572142693E-5	0.0	0.0	1.3046958800499886E-4	6.150709148807088E-4
19	1.8638512572142693E-5	0.0	0.0	1.3046958800499886E-4	6.150709148807088E-4
20	1.8638512572142693E-5	0.0	0.0	1.6774661314928422E-4	6.337094274528515E-4
21	1.8638512572142693E-5	0.0	0.0	2.2366215086571233E-4	6.523479400249942E-4
22	1.8638512572142693E-5	0.0	0.0	2.795776885821404E-4	6.523479400249942E-4
23	1.8638512572142693E-5	0.0	0.0	3.9140876401499655E-4	8.760100908907065E-4
24	1.8638512572142693E-5	0.0	0.0	8.200945531742785E-4	8.946486034628493E-4
25	1.8638512572142693E-5	0.0	0.0	9.878411663235627E-4	9.505641411792773E-4
26	1.8638512572142693E-5	0.0	0.0	0.0013792499303385592	0.001025118191467848
27	1.8638512572142693E-5	0.0	0.0	0.00464098963046353	0.0010623952166121334
28	1.8638512572142693E-5	0.0	0.0	0.010884891342131333	0.0010623952166121334
29	1.8638512572142693E-5	0.0	0.0	0.020315978703635534	0.0010810337291842762
30	1.8638512572142693E-5	0.0	0.0	0.03738885621971824	0.0010810337291842762
31	1.8638512572142693E-5	0.0	0.0	0.13199794603591455	0.0010996722417564188
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3385	0.0	19.838997	1
TTCGCGA	90	4.4508502E-5	16.444445	22
TACTTAC	1665	0.0	15.333333	31
TACGGAT	3135	0.0	14.929825	27
CGTTACG	1610	0.0	14.822982	24
TCGTTAC	1750	0.0	14.694286	23
GTTACGG	2820	0.0	14.3670225	25
ACGGATG	3280	0.0	14.213414	28
TATACTG	990	0.0	14.01515	5
GTATCAA	4910	0.0	13.639511	2
ATTATAC	750	0.0	13.566668	3
GTACTTA	1940	0.0	13.541237	30
TTACGGA	3505	0.0	13.459344	26
TAATCCC	1490	0.0	13.409395	5
CGGATGG	3505	0.0	13.300999	29
TCTTATA	2170	0.0	13.129032	37
TTATACT	960	0.0	13.104166	4
TAATACG	170	3.7388054E-7	13.058824	4
CGAACGT	255	1.6370905E-11	13.058824	4
GGGCGTC	2010	0.0	12.977612	11
>>END_MODULE