FastQCFastQC Report
Thu 9 Feb 2017
SRR2727259.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727259.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7652093
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31450.020.7647061
TATACTG13400.015.3246275
TCGTTAC22700.015.32158623
CGTTACG18500.015.224
TATACCG3150.014.682545
TACTTAC15050.014.50498231
CTCGTTA24900.014.11646622
ACGAACG4500.013.97777715
CCGATAA5100.013.7843159
ACGGATG45350.013.70672528
GTTACGG32750.013.67022825
TACGGAT43850.013.62713727
GTATCAA48200.013.5103732
TCTATAC7300.013.4315073
CGGATGG48350.013.08583429
TTATACT11900.013.0588234
ATTATAC9250.013.03
CGTTACA8050.012.86956524
TTACGGA47650.012.61804926
TTAGACT7350.012.5850334
Produced by FastQC (version 0.11.5)