FastQCFastQC Report
Thu 9 Feb 2017
SRR2727258.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727258.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9033425
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG13750.015.8763645
CCGATAA11000.015.6409099
CGATAAC11300.015.55309710
AACGAAC11350.015.1585914
CGAACGA11350.014.99559516
ACGAACG11950.014.70711315
TAGTACT8950.014.6759784
AAGACGG21450.014.2307695
TATACCG4750.014.0210525
TAACGAA12450.013.81927713
CAAGACG23600.013.796614
GGTATCA51350.013.6183051
GATAACG12650.013.45454611
ATAACGA12800.013.44140712
TCGTTAC7850.013.19745323
TTATACT13900.013.1762594
TAGAGTG14750.013.1694925
TTAGAGT14750.013.1694924
ATTCCGA14300.013.0664346
ATACCGT21250.012.8847066
Produced by FastQC (version 0.11.5)