##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727258.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9033425
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.570110340208725	31.0	31.0	34.0	30.0	34.0
2	31.790581756089193	31.0	31.0	34.0	30.0	34.0
3	31.964723789703243	31.0	31.0	34.0	30.0	34.0
4	35.6721973116509	37.0	35.0	37.0	35.0	37.0
5	35.47337228127759	37.0	35.0	37.0	33.0	37.0
6	35.47898001035045	37.0	35.0	37.0	33.0	37.0
7	35.37874693153483	37.0	35.0	37.0	33.0	37.0
8	35.33558534000116	37.0	35.0	37.0	33.0	37.0
9	36.883107680641615	39.0	37.0	39.0	33.0	39.0
10	36.67427415404456	39.0	35.0	39.0	32.0	39.0
11	36.73213559641	39.0	35.0	39.0	32.0	39.0
12	36.56200444460435	38.0	35.0	39.0	32.0	39.0
13	36.54817679894392	38.0	35.0	39.0	32.0	39.0
14	37.55722032341	39.0	36.0	41.0	32.0	41.0
15	37.47678737577386	39.0	36.0	41.0	32.0	41.0
16	37.39772013383628	39.0	36.0	41.0	32.0	41.0
17	37.71083437345193	39.0	37.0	41.0	32.0	41.0
18	37.73601275263812	39.0	37.0	41.0	32.0	41.0
19	37.81381790406186	40.0	37.0	41.0	32.0	41.0
20	37.775534085908724	40.0	37.0	41.0	32.0	41.0
21	37.72372029435126	40.0	37.0	41.0	32.0	41.0
22	37.65757915740708	40.0	37.0	41.0	32.0	41.0
23	37.53450568306041	39.0	37.0	41.0	32.0	41.0
24	37.407064430157995	39.0	36.0	41.0	31.0	41.0
25	37.32719505613873	39.0	36.0	41.0	31.0	41.0
26	37.03564129884291	39.0	36.0	41.0	31.0	41.0
27	36.84736177031414	39.0	36.0	41.0	30.0	41.0
28	36.64249041753266	39.0	36.0	41.0	30.0	41.0
29	36.47090809964106	39.0	36.0	40.0	30.0	41.0
30	36.32353509327857	39.0	35.0	40.0	29.0	41.0
31	36.1715139053017	39.0	35.0	40.0	28.0	41.0
32	36.02903516661731	39.0	35.0	40.0	27.0	41.0
33	35.85294547749054	38.0	35.0	40.0	27.0	41.0
34	35.72008501758746	38.0	35.0	40.0	26.0	41.0
35	35.55341822177081	38.0	34.0	40.0	26.0	41.0
36	35.46202774695091	38.0	34.0	40.0	25.0	41.0
37	35.38065229965379	38.0	34.0	40.0	25.0	41.0
38	35.262952645314485	38.0	34.0	40.0	25.0	41.0
39	35.12314188693657	38.0	34.0	40.0	24.0	41.0
40	35.02524568477626	38.0	34.0	40.0	24.0	41.0
41	34.81241644226858	38.0	33.0	40.0	23.0	41.0
42	34.63192299709136	38.0	33.0	40.0	23.0	41.0
43	34.344687424758604	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	18.0
11	35.0
12	64.0
13	84.0
14	121.0
15	237.0
16	455.0
17	851.0
18	1608.0
19	3354.0
20	6235.0
21	11691.0
22	20099.0
23	32534.0
24	48513.0
25	67229.0
26	91403.0
27	118345.0
28	153442.0
29	192546.0
30	239020.0
31	296061.0
32	361031.0
33	442497.0
34	544265.0
35	678028.0
36	865895.0
37	1141872.0
38	1773949.0
39	1941940.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.67825879995683	20.307745954607473	12.331557521095265	26.682437724340435
2	19.60901872766974	22.282168723380114	33.36795290822695	24.740859640723205
3	19.111477651057047	24.541333990153234	29.67332988318384	26.673858475605876
4	14.832148382258115	16.11293612334192	34.18186346817514	34.873052026224826
5	13.54451938218339	38.41960275310859	34.27642339422755	13.759454470480465
6	35.09661064324993	37.09807741803358	14.434281570943469	13.37103036777302
7	29.436144098168743	31.231675693327837	20.7996081220578	18.53257208644562
8	27.387131680398074	35.43207587376881	18.843705460553444	18.33708698527967
9	27.698375754489575	13.99183587620421	17.650060746616038	40.65972762269018
10	16.193215751500674	27.1300420383188	33.34720773128686	23.329534478893667
11	37.887800031549496	21.691108300561527	20.43378895601613	19.98730271187285
12	21.67192399339121	25.13020255329512	29.36009320938625	23.837780243927416
13	30.580892629318335	19.788120231252265	24.203145540036033	25.427841599393364
14	22.82695655302391	20.28964650727714	23.968904374586604	32.91449256511235
15	25.27568447183654	28.186474122495063	20.960831578277343	25.577009827391052
16	25.584338166310122	26.223021722104296	22.993571098448264	25.199069013137322
17	24.558282157653384	26.423565812524043	23.963889665326274	25.0542623644963
18	25.04721077553641	25.49185939995074	24.648557994337697	24.812371830175156
19	25.675875982808293	25.369115258055498	24.40759733987939	24.547411419256814
20	25.34455093167874	24.88348549968589	24.342217929522857	25.429745639112518
21	25.646418717153242	25.271976022383534	24.369527615494675	24.71207764496855
22	25.808051763312367	24.686339898764864	24.18716046239383	25.31844787552894
23	25.034159247461513	24.734616161644116	24.42586283718523	25.805361753709143
24	25.187201974887707	25.10175265749148	24.247580513481875	25.463464854138934
25	25.62485436033398	24.62710433750211	24.18979512200522	25.55824618015869
26	25.53802129314186	25.145523431035294	24.228030896365443	25.088424379457404
27	25.921707436548154	25.061612843412107	23.847776452452973	25.168903267586767
28	25.41865350074861	25.132892562898345	24.203566199973984	25.244887736379056
29	25.355964099995294	25.283621660665805	24.345118269095057	25.015295970243844
30	25.54217254252955	25.239341667197102	24.188555282188094	25.02993050808525
31	25.70699374821842	24.789678333522446	24.239499414673837	25.263828503585295
32	25.30610482734954	25.00319646202852	23.952753246968896	25.737945463653045
33	25.163633948363994	24.660668572551387	24.260045331643312	25.91565214744131
34	25.799516794571275	24.780003154949533	24.527430072204066	24.89304997827513
35	25.870420134113033	24.634886546354235	24.45995843215613	25.034734887376604
36	25.319632365354227	24.92949241289987	24.537271300752483	25.21360392099342
37	25.617304621447566	24.56440386675043	24.67613336027033	25.142158151531675
38	25.381414026241433	24.29720731616192	24.799043552141075	25.522335105455575
39	25.49840176898574	24.460290532107145	24.674273600544645	25.367034098362474
40	25.662304164810134	24.17379897436465	25.035288387294962	25.12860847353025
41	24.860260643111555	24.582558664072597	25.275186321910013	25.28199437090583
42	25.357137519822214	24.457832992469633	25.273780432117388	24.911249055590766
43	24.952230189546047	23.853178611656155	25.231648018331914	25.96294318046588
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	212.0
1	230.0
2	248.0
3	275.0
4	302.0
5	302.0
6	415.5
7	529.0
8	714.0
9	899.0
10	1352.0
11	1805.0
12	1805.0
13	3566.0
14	5327.0
15	8276.5
16	11226.0
17	11973.5
18	12721.0
19	12721.0
20	17698.0
21	22675.0
22	27361.0
23	32047.0
24	42074.5
25	52102.0
26	52102.0
27	67378.5
28	82655.0
29	99290.0
30	115925.0
31	143181.5
32	170438.0
33	170438.0
34	206648.0
35	242858.0
36	291527.5
37	340197.0
38	393306.0
39	446415.0
40	446415.0
41	489900.0
42	533385.0
43	577938.5
44	622492.0
45	673384.5
46	724277.0
47	724277.0
48	779071.5
49	833866.0
50	840266.5
51	846667.0
52	825957.5
53	805248.0
54	805248.0
55	774935.0
56	744622.0
57	699679.5
58	654737.0
59	600610.5
60	546484.0
61	546484.0
62	471170.5
63	395857.0
64	340184.0
65	284511.0
66	236457.0
67	188403.0
68	188403.0
69	152264.5
70	116126.0
71	97894.0
72	79662.0
73	63884.0
74	48106.0
75	48106.0
76	37298.5
77	26491.0
78	22147.5
79	17804.0
80	13895.5
81	9987.0
82	9987.0
83	8166.5
84	6346.0
85	5074.0
86	3802.0
87	3403.0
88	3004.0
89	3004.0
90	2109.0
91	1214.0
92	976.0
93	738.0
94	710.0
95	682.0
96	682.0
97	417.5
98	153.0
99	166.5
100	180.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	9033425.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.489426063983714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.65011678584442	39.41838565660716
2	9.827711236058539	9.727355771902022
3	3.6199278687885457	5.374444578580947
4	1.872544324182067	3.70684575532563
5	1.1181665035618988	2.766870925262489
6	0.7595421183775052	2.2553582105955066
7	0.500156549984566	1.732672242061485
8	0.3803589284097707	1.5058996052249138
9	0.2875527270186102	1.2807737480955963
>10	1.7590434989667905	16.41321285509995
>50	0.1475257942788656	5.024119972941574
>100	0.07017749635162424	6.144551734038896
>500	0.0035550712478177966	1.2126275699755336
>1k	0.0035086405373914416	3.1208221787269586
>5k	1.1245639167881497E-4	0.31605919556133677
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.106999836717524E-5	0.0
3	0.0	0.0	0.0	1.106999836717524E-5	0.0
4	0.0	0.0	0.0	2.213999673435048E-5	0.0
5	0.0	0.0	0.0	3.320999510152572E-5	0.0
6	1.106999836717524E-5	0.0	0.0	3.320999510152572E-5	1.106999836717524E-5
7	1.106999836717524E-5	0.0	0.0	3.320999510152572E-5	2.213999673435048E-5
8	1.106999836717524E-5	0.0	0.0	4.427999346870096E-5	3.320999510152572E-5
9	1.106999836717524E-5	0.0	0.0	5.53499918358762E-5	5.53499918358762E-5
10	1.106999836717524E-5	0.0	0.0	7.748998857022669E-5	5.53499918358762E-5
11	1.106999836717524E-5	0.0	0.0	7.748998857022669E-5	5.53499918358762E-5
12	1.106999836717524E-5	0.0	0.0	8.855998693740192E-5	5.53499918358762E-5
13	1.106999836717524E-5	0.0	0.0	8.855998693740192E-5	5.53499918358762E-5
14	1.106999836717524E-5	0.0	0.0	8.855998693740192E-5	9.962998530457716E-5
15	2.213999673435048E-5	1.106999836717524E-5	0.0	8.855998693740192E-5	1.106999836717524E-4
16	4.427999346870096E-5	1.106999836717524E-5	0.0	1.106999836717524E-4	2.1032996897632958E-4
17	4.427999346870096E-5	1.106999836717524E-5	0.0	1.2176998203892765E-4	2.1032996897632958E-4
18	4.427999346870096E-5	1.106999836717524E-5	0.0	1.2176998203892765E-4	2.5460996244503056E-4
19	4.427999346870096E-5	1.106999836717524E-5	0.0	1.5497997714045337E-4	2.76749959179381E-4
20	4.427999346870096E-5	1.106999836717524E-5	0.0	1.5497997714045337E-4	2.76749959179381E-4
21	4.427999346870096E-5	1.106999836717524E-5	0.0	1.6604997550762862E-4	3.0995995428090674E-4
22	4.427999346870096E-5	1.106999836717524E-5	0.0	2.213999673435048E-4	3.2102995264808197E-4
23	4.427999346870096E-5	1.106999836717524E-5	0.0	3.2102995264808197E-4	4.870799281557106E-4
24	4.427999346870096E-5	1.106999836717524E-5	0.0	5.202899232572363E-4	5.202899232572363E-4
25	5.53499918358762E-5	1.106999836717524E-5	0.0	6.199199085618135E-4	5.313599216244116E-4
26	5.53499918358762E-5	1.106999836717524E-5	0.0	9.077398661083698E-4	5.424299199915868E-4
27	5.53499918358762E-5	1.106999836717524E-5	0.0	0.0028228495836296864	5.424299199915868E-4
28	5.53499918358762E-5	1.106999836717524E-5	0.0	0.006498089041531867	5.424299199915868E-4
29	5.53499918358762E-5	1.106999836717524E-5	0.0	0.012785848114087403	5.424299199915868E-4
30	5.53499918358762E-5	1.106999836717524E-5	0.0	0.024232226425746604	5.424299199915868E-4
31	5.53499918358762E-5	1.106999836717524E-5	0.0	0.08264860780933035	5.53499918358762E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACTG	1375	0.0	15.876364	5
CCGATAA	1100	0.0	15.640909	9
CGATAAC	1130	0.0	15.553097	10
AACGAAC	1135	0.0	15.15859	14
CGAACGA	1135	0.0	14.995595	16
ACGAACG	1195	0.0	14.707113	15
TAGTACT	895	0.0	14.675978	4
AAGACGG	2145	0.0	14.230769	5
TATACCG	475	0.0	14.021052	5
TAACGAA	1245	0.0	13.819277	13
CAAGACG	2360	0.0	13.79661	4
GGTATCA	5135	0.0	13.618305	1
GATAACG	1265	0.0	13.454546	11
ATAACGA	1280	0.0	13.441407	12
TCGTTAC	785	0.0	13.197453	23
TTATACT	1390	0.0	13.176259	4
TAGAGTG	1475	0.0	13.169492	5
TTAGAGT	1475	0.0	13.169492	4
ATTCCGA	1430	0.0	13.066434	6
ATACCGT	2125	0.0	12.884706	6
>>END_MODULE