FastQCFastQC Report
Thu 9 Feb 2017
SRR2727257.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727257.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7580497
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG10150.014.9458138
ACCGTCC6950.014.9064758
TAATACT10150.014.7635474
TACCGTC11200.014.7008927
AAGACGG15550.014.514475
TAGTACT6500.014.2307694
TTAGAGT11000.014.1272744
ATACCGT12850.014.108956
TATACCG3950.014.0506325
CAAGACG16350.013.3516824
TTAGTAC4350.013.18390753
ACGGACC15600.013.1634628
CGTCGTA11550.012.97402610
GTAATAC6850.012.6934313
CCGTCGT11100.012.6666679
GTATTAG11250.012.6622221
CCTATAC7600.012.6578953
GACGGAC16850.012.6261147
ATTATAC8700.012.5459773
CGCAAGA18350.012.5013632
Produced by FastQC (version 0.11.5)