##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727257.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7580497
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.575366496418376	31.0	31.0	34.0	30.0	34.0
2	31.79256069885655	31.0	31.0	34.0	30.0	34.0
3	31.96795262896351	31.0	31.0	34.0	30.0	34.0
4	35.672180729047184	37.0	35.0	37.0	35.0	37.0
5	35.47224885122967	37.0	35.0	37.0	33.0	37.0
6	35.483102756982824	37.0	35.0	37.0	33.0	37.0
7	35.389052723060246	37.0	35.0	37.0	33.0	37.0
8	35.347698970133486	37.0	35.0	37.0	33.0	37.0
9	36.89616841745337	39.0	37.0	39.0	33.0	39.0
10	36.69050564890402	39.0	35.0	39.0	32.0	39.0
11	36.76112186311794	39.0	35.0	39.0	32.0	39.0
12	36.59139341391468	38.0	35.0	39.0	32.0	39.0
13	36.584782237892846	38.0	35.0	39.0	32.0	39.0
14	37.58681192011553	39.0	36.0	41.0	32.0	41.0
15	37.51975787339537	39.0	36.0	41.0	32.0	41.0
16	37.43453285450809	39.0	36.0	41.0	32.0	41.0
17	37.74842586178716	39.0	37.0	41.0	32.0	41.0
18	37.76985532742774	40.0	37.0	41.0	32.0	41.0
19	37.85598648742952	40.0	37.0	41.0	32.0	41.0
20	37.82090593796159	40.0	37.0	41.0	32.0	41.0
21	37.77809462888779	40.0	37.0	41.0	32.0	41.0
22	37.7189225191963	40.0	37.0	41.0	32.0	41.0
23	37.608072003722185	40.0	37.0	41.0	32.0	41.0
24	37.50291478250041	39.0	37.0	41.0	32.0	41.0
25	37.41786785220019	39.0	36.0	41.0	31.0	41.0
26	37.153154206116035	39.0	36.0	41.0	31.0	41.0
27	36.97830603982826	39.0	36.0	41.0	30.0	41.0
28	36.79061755449544	39.0	36.0	41.0	30.0	41.0
29	36.63330016488365	39.0	36.0	40.0	30.0	41.0
30	36.498888925092906	39.0	36.0	40.0	30.0	41.0
31	36.34955029993416	39.0	35.0	40.0	29.0	41.0
32	36.20457088763441	39.0	35.0	40.0	29.0	41.0
33	36.04602468677186	39.0	35.0	40.0	28.0	41.0
34	35.922123971554896	38.0	35.0	40.0	27.0	41.0
35	35.76788408464511	38.0	35.0	40.0	27.0	41.0
36	35.68068492079081	38.0	35.0	40.0	26.0	41.0
37	35.6155217791129	38.0	35.0	40.0	26.0	41.0
38	35.51603107289667	38.0	34.0	40.0	26.0	41.0
39	35.370612771167906	38.0	34.0	40.0	25.0	41.0
40	35.29659664795066	38.0	34.0	40.0	25.0	41.0
41	35.09148780086583	38.0	34.0	40.0	24.0	41.0
42	34.93009007193064	38.0	34.0	40.0	24.0	41.0
43	34.65308171746523	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	21.0
12	42.0
13	55.0
14	85.0
15	143.0
16	339.0
17	594.0
18	1122.0
19	2209.0
20	4611.0
21	8453.0
22	14815.0
23	24122.0
24	36373.0
25	50944.0
26	69370.0
27	92330.0
28	120412.0
29	152346.0
30	193064.0
31	240905.0
32	298157.0
33	364425.0
34	453975.0
35	568856.0
36	728339.0
37	966985.0
38	1517478.0
39	1669919.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.68931100427848	20.069554806235	12.440081435293754	26.801052754192767
2	19.392488381698456	22.0955565314517	33.87768638388749	24.634268702962352
3	19.20338468572707	24.453185589282604	29.785276611810545	26.558153113179785
4	14.97949276940549	16.079750443803356	34.1166548842378	34.82410190255336
5	13.526975869787957	38.51709195320571	34.214498073147446	13.741434103858891
6	35.1505712620162	36.854008384938346	14.52809756405154	13.467322788993913
7	29.206172101908358	31.400685205732554	20.791064227055298	18.60207846530379
8	27.23011433155372	35.92433319345684	18.858380921462008	17.987171553527425
9	28.022595352257245	13.916541356061481	17.516991300174645	40.54387199150663
10	15.952977753305623	27.334883187738217	33.536877595228916	23.175261463727246
11	37.79782512940774	21.654582806378	20.376816981788927	20.170775082425337
12	21.61529778324561	24.95056722534156	29.561940331880614	23.872194659532216
13	30.71210238589897	19.92151701926668	24.032395237409894	25.333985357424453
14	22.705054826880083	20.239504085286228	24.212871530718896	32.84256955711479
15	25.506823629110336	28.091522231325992	21.087588320396407	25.314065819167265
16	25.242869959581803	26.418122716755903	23.354801142985743	24.984206180676544
17	24.574958607595253	26.23573362010433	24.028609205966312	25.160698566334105
18	24.754669779567223	25.850429068173238	24.81098534832215	24.58391580393739
19	25.706810516513627	25.510965837728055	24.6148372593512	24.16738638640712
20	25.13184821522916	24.94125385182528	24.544709931288146	25.38218800165741
21	25.276654024135887	25.353627868990646	24.956648620796233	24.413069486077234
22	25.589351199532167	24.723563639692753	24.241022719222762	25.446062441552314
23	24.845376233246977	24.93909040528609	24.78879682954825	25.426736531918685
24	24.88216801616042	25.114698943881912	24.626168970187575	25.376964069770096
25	25.452539589422702	24.606196664941628	24.33348367527881	25.607780070356867
26	25.26261800512552	25.111189939129318	24.602028072829526	25.02416398291563
27	25.853990839914587	25.155566976677125	24.02302909690486	24.96741308650343
28	25.053884989335135	25.575301988774612	24.116255174297937	25.254557847592313
29	25.290004072292362	25.460599746955904	24.508894337666778	24.740501843084957
30	25.731188865321098	25.238793709700037	24.170776665435	24.859240759543866
31	25.702866184103762	24.816974401546496	24.44826506758066	25.031894346769086
32	25.120107560229886	25.2720632961137	24.197318460781663	25.410510682874747
33	25.0406140916618	24.69427796093053	24.437223575182472	25.827884372225196
34	25.55344326368047	24.910345588158666	24.808069972193117	24.72814117596775
35	25.616763650193384	24.89872365888411	24.633899334041025	24.85061335688148
36	25.309237639695652	24.970869324267262	24.73296935543936	24.986923680597723
37	25.258475796507803	24.906981692625166	25.03579910393738	24.798743406929653
38	25.610932897935317	24.348918019491332	24.82706608814699	25.213082994426355
39	25.10488428397241	24.749142437494534	25.04965043848708	25.096322840045975
40	25.71037228825498	24.20894039005622	25.208756101347973	24.87193122034083
41	24.72729690414758	24.765091259847473	25.257591949446056	25.25001988655889
42	25.11359083711794	24.770038165043793	25.302523040375846	24.81384795746242
43	24.77308545864473	24.159458146345813	25.214666004089175	25.852790390920276
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	202.0
1	245.5
2	289.0
3	331.5
4	374.0
5	374.0
6	502.5
7	631.0
8	814.5
9	998.0
10	1463.0
11	1928.0
12	1928.0
13	3777.5
14	5627.0
15	8980.0
16	12333.0
17	12880.0
18	13427.0
19	13427.0
20	17760.0
21	22093.0
22	26012.0
23	29931.0
24	38681.5
25	47432.0
26	47432.0
27	61173.0
28	74914.0
29	90101.0
30	105288.0
31	127465.5
32	149643.0
33	149643.0
34	179009.0
35	208375.0
36	247390.5
37	286406.0
38	326328.0
39	366250.0
40	366250.0
41	402485.5
42	438721.0
43	477854.5
44	516988.0
45	562758.5
46	608529.0
47	608529.0
48	659255.5
49	709982.0
50	722175.0
51	734368.0
52	711354.5
53	688341.0
54	688341.0
55	655804.5
56	623268.0
57	583462.5
58	543657.0
59	494656.0
60	445655.0
61	445655.0
62	382584.0
63	319513.0
64	272640.5
65	225768.0
66	187524.5
67	149281.0
68	149281.0
69	121265.5
70	93250.0
71	78073.0
72	62896.0
73	50465.0
74	38034.0
75	38034.0
76	29876.5
77	21719.0
78	17979.5
79	14240.0
80	11173.5
81	8107.0
82	8107.0
83	6533.0
84	4959.0
85	3902.0
86	2845.0
87	2507.5
88	2170.0
89	2170.0
90	1530.5
91	891.0
92	708.5
93	526.0
94	493.0
95	460.0
96	460.0
97	268.0
98	76.0
99	94.0
100	112.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	7580497.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.09309306445752
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.90936822637127	41.62726155738364
2	9.949910554761269	10.366432330244141
3	3.5918383445284934	5.613299074620294
4	1.7783413550683576	3.7055720683979785
5	1.0558685430146268	2.7501729137547057
6	0.7018018883158614	2.1935418648510114
7	0.5096377344421497	1.8584024150617933
8	0.3707826088547783	1.5452170359803457
9	0.2896685722092068	1.3580758700948468
>10	1.6607618872845002	16.05987045495008
>50	0.1218995401734212	4.343190877263417
>100	0.05367647019903111	5.001127065709354
>500	0.004355754237672222	1.5962493470848211
>1k	0.002063050777659068	1.909475616728409
>5k	2.546976182263992E-5	0.07211150787517756
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.276698876076331E-5
2	0.0	0.0	0.0	0.0	5.276698876076331E-5
3	0.0	0.0	0.0	0.0	5.276698876076331E-5
4	0.0	0.0	0.0	1.3191747190190828E-5	5.276698876076331E-5
5	0.0	0.0	0.0	2.6383494380381656E-5	5.276698876076331E-5
6	0.0	0.0	0.0	2.6383494380381656E-5	1.319174719019083E-4
7	0.0	0.0	0.0	3.9575241570572485E-5	1.451092190920991E-4
8	0.0	0.0	0.0	3.9575241570572485E-5	1.846844606626716E-4
9	0.0	0.0	0.0	7.915048314114497E-5	4.089441628959157E-4
10	0.0	0.0	0.0	9.23422303313358E-5	4.089441628959157E-4
11	0.0	0.0	0.0	9.23422303313358E-5	4.221359100861065E-4
12	0.0	0.0	0.0	9.23422303313358E-5	4.221359100861065E-4
13	0.0	0.0	0.0	9.23422303313358E-5	4.221359100861065E-4
14	0.0	0.0	0.0	9.23422303313358E-5	4.749028988468698E-4
15	0.0	0.0	0.0	9.23422303313358E-5	4.880946460370606E-4
16	3.9575241570572485E-5	0.0	0.0	1.1872572471171745E-4	5.936286235585873E-4
17	3.9575241570572485E-5	0.0	0.0	1.451092190920991E-4	6.068203707487781E-4
18	3.9575241570572485E-5	0.0	0.0	1.451092190920991E-4	6.85970853889923E-4
19	3.9575241570572485E-5	0.0	0.0	1.5830096628228994E-4	7.255460954604955E-4
20	3.9575241570572485E-5	1.3191747190190828E-5	0.0	1.5830096628228994E-4	7.255460954604955E-4
21	3.9575241570572485E-5	1.3191747190190828E-5	0.0	2.2425970223324408E-4	7.65121337031068E-4
22	3.9575241570572485E-5	1.3191747190190828E-5	0.0	3.0341018537438907E-4	7.783130842212589E-4
23	3.9575241570572485E-5	1.3191747190190828E-5	0.0	4.353276572762973E-4	9.761892920741212E-4
24	3.9575241570572485E-5	1.3191747190190828E-5	0.0	5.672451291782056E-4	9.761892920741212E-4
25	3.9575241570572485E-5	1.3191747190190828E-5	0.0	6.991626010801139E-4	9.761892920741212E-4
26	3.9575241570572485E-5	1.3191747190190828E-5	0.0	9.893810392643122E-4	9.761892920741212E-4
27	3.9575241570572485E-5	1.3191747190190828E-5	0.0	0.003218786314406562	9.893810392643122E-4
28	3.9575241570572485E-5	1.3191747190190828E-5	0.0	0.006318846904101407	9.893810392643122E-4
29	3.9575241570572485E-5	1.3191747190190828E-5	0.0	0.011872572471171745	0.0010025727864545028
30	5.276698876076331E-5	1.3191747190190828E-5	0.0	0.022214902268281354	0.0010025727864545028
31	5.276698876076331E-5	1.3191747190190828E-5	0.0	0.07999475496131718	0.0010289562808348846
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTCG	1015	0.0	14.945813	8
ACCGTCC	695	0.0	14.906475	8
TAATACT	1015	0.0	14.763547	4
TACCGTC	1120	0.0	14.700892	7
AAGACGG	1555	0.0	14.51447	5
TAGTACT	650	0.0	14.230769	4
TTAGAGT	1100	0.0	14.127274	4
ATACCGT	1285	0.0	14.10895	6
TATACCG	395	0.0	14.050632	5
CAAGACG	1635	0.0	13.351682	4
TTAGTAC	435	0.0	13.1839075	3
ACGGACC	1560	0.0	13.163462	8
CGTCGTA	1155	0.0	12.974026	10
GTAATAC	685	0.0	12.693431	3
CCGTCGT	1110	0.0	12.666667	9
GTATTAG	1125	0.0	12.662222	1
CCTATAC	760	0.0	12.657895	3
GACGGAC	1685	0.0	12.626114	7
ATTATAC	870	0.0	12.545977	3
CGCAAGA	1835	0.0	12.501363	2
>>END_MODULE