FastQCFastQC Report
Thu 9 Feb 2017
SRR2727256.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727256.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5879197
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT65610.11159687283824646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG17100.017.9590645
CAAGACG17300.017.644514
CGAACGT5450.017.3119264
TATACCG3200.015.6093755
CGTTACG5850.015.17948724
CGAACGA7950.014.89308116
ACGAACG7950.014.89308115
TCGAACG6350.014.8582683
GACGGAC19100.014.8193717
ACGGACC19250.014.5116878
CGCAAGA20200.014.2871292
CCGATAA8450.014.230779
CGCTTCG11950.014.08786732
ACCGTCG13300.013.9097748
TATCTAG14200.013.8098591
ATACCGT14900.013.781886
GCGCAAG21100.013.7654041
TAGAGTG11850.013.7383975
GTATTAG14550.013.7319591
CGGACCA20750.013.5518079
Produced by FastQC (version 0.11.5)