##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2727256.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5879197 Sequences flagged as poor quality 0 Sequence length 43 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.49888037430962 31.0 31.0 34.0 30.0 34.0 2 31.709264717613646 31.0 31.0 34.0 30.0 34.0 3 31.88200854640523 31.0 31.0 34.0 30.0 34.0 4 35.59912688756645 37.0 35.0 37.0 33.0 37.0 5 35.39506908851668 37.0 35.0 37.0 33.0 37.0 6 35.40510073059297 37.0 35.0 37.0 33.0 37.0 7 35.301602412710444 37.0 35.0 37.0 32.0 37.0 8 35.26284354819204 37.0 35.0 37.0 32.0 37.0 9 36.79435814108628 39.0 37.0 39.0 32.0 39.0 10 36.58897465078989 39.0 35.0 39.0 32.0 39.0 11 36.643996960809446 39.0 35.0 39.0 32.0 39.0 12 36.47672020515727 38.0 35.0 39.0 32.0 39.0 13 36.462577797614195 38.0 35.0 39.0 32.0 39.0 14 37.442499035157354 39.0 36.0 41.0 32.0 41.0 15 37.36742857910698 39.0 36.0 41.0 32.0 41.0 16 37.282736230815196 39.0 36.0 41.0 31.0 41.0 17 37.59909848232675 39.0 36.0 41.0 32.0 41.0 18 37.63215469731666 39.0 37.0 41.0 32.0 41.0 19 37.703806999493295 40.0 37.0 41.0 32.0 41.0 20 37.66781109733183 40.0 37.0 41.0 32.0 41.0 21 37.61538778169876 40.0 37.0 41.0 32.0 41.0 22 37.54780338199247 39.0 37.0 41.0 32.0 41.0 23 37.42059978599118 39.0 36.0 41.0 31.0 41.0 24 37.29109213384073 39.0 36.0 41.0 31.0 41.0 25 37.20264927336165 39.0 36.0 41.0 31.0 41.0 26 36.90422603631074 39.0 36.0 41.0 30.0 41.0 27 36.70951066956933 39.0 36.0 41.0 30.0 41.0 28 36.48297735217922 39.0 36.0 40.0 30.0 41.0 29 36.29474909583741 39.0 35.0 40.0 29.0 41.0 30 36.14939795349603 39.0 35.0 40.0 28.0 41.0 31 35.98965743110836 39.0 35.0 40.0 27.0 41.0 32 35.8257440599456 38.0 35.0 40.0 27.0 41.0 33 35.64897281040251 38.0 35.0 40.0 26.0 41.0 34 35.51387987168996 38.0 34.0 40.0 26.0 41.0 35 35.33651942603726 38.0 34.0 40.0 25.0 41.0 36 35.2287252834018 38.0 34.0 40.0 25.0 41.0 37 35.14805032047744 38.0 34.0 40.0 24.0 41.0 38 35.02100014678875 38.0 34.0 40.0 24.0 41.0 39 34.881099748826244 38.0 33.0 40.0 24.0 41.0 40 34.768188240673005 38.0 33.0 40.0 23.0 41.0 41 34.5423073933396 38.0 33.0 40.0 23.0 41.0 42 34.356908264853175 38.0 33.0 40.0 22.0 41.0 43 34.07659158214974 38.0 33.0 40.0 21.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 15.0 11 32.0 12 57.0 13 56.0 14 113.0 15 218.0 16 369.0 17 691.0 18 1276.0 19 2497.0 20 4722.0 21 8574.0 22 14631.0 23 23314.0 24 34572.0 25 47993.0 26 64059.0 27 84066.0 28 107855.0 29 134046.0 30 166374.0 31 202901.0 32 246465.0 33 297137.0 34 362787.0 35 444730.0 36 561549.0 37 732421.0 38 1127398.0 39 1208276.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.60648078300489 20.418621794779117 12.346839202700641 26.628058219515356 2 19.654758974737536 22.505879629480013 33.27952779945969 24.559833596322765 3 19.139994798609404 24.866133929514525 29.81817074678736 26.175700525088715 4 14.652324118412768 16.201719384466962 34.41823772872384 34.72771876839643 5 13.380602827222834 38.72940471292253 34.24809544568757 13.641897014167071 6 34.74814672820115 37.47573010395808 14.572517301257298 13.203605866583482 7 29.108209845664295 31.511106023492662 21.023126117393243 18.357558013449797 8 27.40151418637613 35.41612910742742 18.96565126155834 18.216705444638105 9 27.881987965363297 13.822874110188858 17.630094722119363 40.665043202328484 10 16.0416635128913 27.191383449134293 33.30868144067974 23.458271597294665 11 37.875495582134775 21.766577986755674 20.49426137617093 19.863665054938625 12 21.669762044034243 25.307826902211307 29.317541154004534 23.704869899749916 13 30.328104331254764 19.733902435995937 24.404234115645384 25.533759117103916 14 22.72919243903547 20.53612083418875 23.981965564344925 32.75272116243085 15 25.345655197469995 28.25797468599879 20.96801655055954 25.42835356597168 16 25.424033248077926 26.352595430974674 23.170647283974326 25.05272403697308 17 24.509843776284416 26.472866957851558 23.944732588481045 25.072556677382984 18 25.0514143343045 25.40654786699612 24.799100965659086 24.742936833040293 19 25.71703244507711 25.410170810741672 24.38659565243349 24.486201091747734 20 25.195804801233912 24.770695725963936 24.392327727749212 25.641171745052937 21 25.676890908741452 25.37713908889258 24.489602916860925 24.456367085505043 22 25.636017980006454 24.699223380335784 24.19014025214668 25.474618387511082 23 24.68736801981631 24.688218476094608 24.69318514075987 25.93122836332921 24 24.979142559774743 25.135949688367305 24.432792437470628 25.452115314387324 25 25.452693624656565 24.568372177356874 24.24310666915907 25.735827528827492 26 25.422349344646893 25.182792820175955 24.365725455364057 25.029132379813095 27 25.97441453314118 25.037415823963716 23.849192330177065 25.138977312718048 28 25.133993638927222 25.36251124090586 24.272549465513745 25.23094565465318 29 25.2592148213438 25.33893659287144 24.502019578524074 24.899829007260685 30 25.491763586081568 25.30047895996681 24.289116353814986 24.918641100136636 31 25.78270467888727 24.832676979526287 24.17974767642588 25.204870665160566 32 25.119910082958608 25.132190671617227 23.951349138326204 25.79655010709796 33 25.009180675524224 24.53290815055185 24.421770524103888 26.036140649820034 34 25.713052309694675 24.87691771512334 24.703254543094914 24.70677543208707 35 26.005626278554704 24.664303645548873 24.527295139115086 24.802774936781333 36 25.216981162563528 25.025934664206694 24.63440160280392 25.12268257042586 37 25.481507083365294 24.710109220697994 24.859024115027953 24.94935958090875 38 25.400764764303695 24.192402465846953 25.018807840594558 25.388024929254794 39 25.492852170117793 24.364943035588023 24.764334313002266 25.37787048129192 40 25.73006143526063 24.270423324817997 25.080346856892195 24.919168383029177 41 24.675444622794576 24.68371105781963 25.427196945433195 25.213647373952597 42 25.51722624705381 24.558676975784277 25.409269327086676 24.51482745007524 43 24.866967376667258 23.909081461294797 25.248380008358286 25.97557115367966 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 199.0 1 221.0 2 243.0 3 284.5 4 326.0 5 326.0 6 467.5 7 609.0 8 822.0 9 1035.0 10 1460.5 11 1886.0 12 1886.0 13 3797.0 14 5708.0 15 8977.5 16 12247.0 17 12096.5 18 11946.0 19 11946.0 20 15741.5 21 19537.0 22 21902.5 23 24268.0 24 31072.0 25 37876.0 26 37876.0 27 47845.0 28 57814.0 29 69176.0 30 80538.0 31 96154.5 32 111771.0 33 111771.0 34 135301.5 35 158832.0 36 191515.0 37 224198.0 38 258642.5 39 293087.0 40 293087.0 41 318448.5 42 343810.0 43 367891.0 44 391972.0 45 427921.0 46 463870.0 47 463870.0 48 504375.0 49 544880.0 50 550050.5 51 555221.0 52 535568.5 53 515916.0 54 515916.0 55 494536.5 56 473157.0 57 447811.0 58 422465.0 59 388920.0 60 355375.0 61 355375.0 62 304302.5 63 253230.0 64 217563.0 65 181896.0 66 152415.0 67 122934.0 68 122934.0 69 99435.5 70 75937.0 71 65584.0 72 55231.0 73 43910.5 74 32590.0 75 32590.0 76 25099.0 77 17608.0 78 14976.5 79 12345.0 80 9470.5 81 6596.0 82 6596.0 83 5470.0 84 4344.0 85 3525.0 86 2706.0 87 2600.5 88 2495.0 89 2495.0 90 1750.5 91 1006.0 92 815.0 93 624.0 94 612.0 95 600.0 96 600.0 97 353.0 98 106.0 99 134.5 100 163.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 5879197.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.52360597041983 #Duplication Level Percentage of deduplicated Percentage of total 1 81.67998837837419 42.901275252541964 2 9.239594740589803 9.705936669622044 3 3.2917544451920904 5.1868444029194185 4 1.5524506983548123 3.2616123507556503 5 0.9496883908951648 2.494052941902984 6 0.6224138443961807 1.9614851708159515 7 0.4336283081140945 1.594300567510315 8 0.3091684954287294 1.29909153858929 9 0.24470447992367728 1.1567485514436961 >10 1.509742371790596 14.743749439388147 >50 0.10448083588319587 3.7405764032755853 >100 0.052197153653894636 5.178343347108532 >500 0.005338060064578244 1.986055334446314 >1k 0.004784699388263507 4.584093446647136 >5k 6.509795085429393E-5 0.20583458303319188 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT 6561 0.11159687283824646 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.401825113191478E-5 2 0.0 0.0 0.0 0.0 3.401825113191478E-5 3 0.0 0.0 0.0 0.0 3.401825113191478E-5 4 0.0 0.0 0.0 0.0 3.401825113191478E-5 5 0.0 0.0 0.0 0.0 3.401825113191478E-5 6 0.0 0.0 0.0 0.0 1.0205475339574435E-4 7 0.0 0.0 0.0 1.700912556595739E-5 1.0205475339574435E-4 8 0.0 0.0 0.0 1.700912556595739E-5 1.3607300452765913E-4 9 0.0 0.0 0.0 3.401825113191478E-5 4.082190135829774E-4 10 0.0 0.0 0.0 6.803650226382956E-5 4.252281391489348E-4 11 0.0 0.0 0.0 1.0205475339574435E-4 4.252281391489348E-4 12 0.0 0.0 0.0 1.1906387896170174E-4 4.76255515846807E-4 13 0.0 0.0 0.0 1.1906387896170174E-4 4.76255515846807E-4 14 0.0 0.0 0.0 1.1906387896170174E-4 6.633558970723383E-4 15 0.0 1.700912556595739E-5 0.0 1.3607300452765913E-4 6.633558970723383E-4 16 0.0 1.700912556595739E-5 0.0 1.5308213009361654E-4 8.334471527319122E-4 17 0.0 1.700912556595739E-5 0.0 2.211186323574461E-4 8.67465403863827E-4 18 0.0 1.700912556595739E-5 0.0 2.381277579234035E-4 0.0010035384083914862 19 0.0 1.700912556595739E-5 0.0 2.5513688348936087E-4 0.0010035384083914862 20 0.0 1.700912556595739E-5 0.0 3.5719163688510523E-4 0.0010205475339574435 21 0.0 1.700912556595739E-5 0.0 4.76255515846807E-4 0.001037556659523401 22 0.0 1.700912556595739E-5 0.0 5.613011436765939E-4 0.001088584036221273 23 0.0 1.700912556595739E-5 0.0 7.143832737702105E-4 0.0013097026685787191 24 0.0 1.700912556595739E-5 0.0 9.865292828255287E-4 0.0013267117941446766 25 0.0 1.700912556595739E-5 0.0 0.0011906387896170173 0.0013947482964085062 26 0.0 1.700912556595739E-5 0.0 0.00156483955206808 0.0014117574219744634 27 0.0 1.700912556595739E-5 0.0 0.004031162759131902 0.0014117574219744634 28 0.0 1.700912556595739E-5 0.0 0.009678192447029756 0.0014117574219744634 29 0.0 1.700912556595739E-5 0.0 0.016992116440391433 0.0014117574219744634 30 0.0 1.700912556595739E-5 0.0 0.03012316137731054 0.0014117574219744634 31 0.0 1.700912556595739E-5 0.0 0.09671388796803373 0.0014797939242382932 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGACGG 1710 0.0 17.959064 5 CAAGACG 1730 0.0 17.64451 4 CGAACGT 545 0.0 17.311926 4 TATACCG 320 0.0 15.609375 5 CGTTACG 585 0.0 15.179487 24 CGAACGA 795 0.0 14.893081 16 ACGAACG 795 0.0 14.893081 15 TCGAACG 635 0.0 14.858268 3 GACGGAC 1910 0.0 14.819371 7 ACGGACC 1925 0.0 14.511687 8 CGCAAGA 2020 0.0 14.287129 2 CCGATAA 845 0.0 14.23077 9 CGCTTCG 1195 0.0 14.087867 32 ACCGTCG 1330 0.0 13.909774 8 TATCTAG 1420 0.0 13.809859 1 ATACCGT 1490 0.0 13.78188 6 GCGCAAG 2110 0.0 13.765404 1 TAGAGTG 1185 0.0 13.738397 5 GTATTAG 1455 0.0 13.731959 1 CGGACCA 2075 0.0 13.551807 9 >>END_MODULE