FastQCFastQC Report
Thu 9 Feb 2017
SRR2727255.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727255.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6634594
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT68320.10297540437289757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACG16650.016.4444454
AAGACGG16500.015.2484865
TATACTG11950.014.8619255
TAACGAA6750.014.25185113
CGCTTCG10000.014.24532
TATACCG3250.014.2307695
GTATTAG13050.013.892721
CGCAAGA17950.013.8105852
GACGGAC17600.013.6647737
CGATAAC6650.013.63157810
ACGGACC17300.013.5809248
AACGCTT10000.013.50530
ATAACGA7000.013.47857212
TAGTACT7700.013.4545454
TTATACT12700.013.4015754
GCGCAAG18450.013.3360431
GTAAACG10550.013.32701527
TCGTTAC7450.013.16107423
CCGATAA6950.013.0431659
CGAACGT3850.012.9740264
Produced by FastQC (version 0.11.5)