##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727255.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6634594
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.519631042984695	31.0	31.0	34.0	30.0	34.0
2	31.728327008404733	31.0	31.0	34.0	30.0	34.0
3	31.90835701476232	31.0	31.0	34.0	30.0	34.0
4	35.616543679990066	37.0	35.0	37.0	33.0	37.0
5	35.41483955159879	37.0	35.0	37.0	33.0	37.0
6	35.43066448376494	37.0	35.0	37.0	33.0	37.0
7	35.32877912348518	37.0	35.0	37.0	33.0	37.0
8	35.29438817205695	37.0	35.0	37.0	32.0	37.0
9	36.83476110218651	39.0	37.0	39.0	33.0	39.0
10	36.628482164846865	39.0	35.0	39.0	32.0	39.0
11	36.688220560293516	39.0	35.0	39.0	32.0	39.0
12	36.520827348289885	38.0	35.0	39.0	32.0	39.0
13	36.510270108464816	38.0	35.0	39.0	32.0	39.0
14	37.50294366166189	39.0	36.0	41.0	32.0	41.0
15	37.42912060632497	39.0	36.0	41.0	32.0	41.0
16	37.35357596862747	39.0	36.0	41.0	32.0	41.0
17	37.66017995976845	39.0	37.0	41.0	32.0	41.0
18	37.69533644409891	39.0	37.0	41.0	32.0	41.0
19	37.772366176438226	40.0	37.0	41.0	32.0	41.0
20	37.7388200393272	40.0	37.0	41.0	32.0	41.0
21	37.691792293545014	40.0	37.0	41.0	32.0	41.0
22	37.62876793968101	40.0	37.0	41.0	32.0	41.0
23	37.512454115504276	39.0	37.0	41.0	32.0	41.0
24	37.3997438275801	39.0	36.0	41.0	31.0	41.0
25	37.32383338000788	39.0	36.0	41.0	31.0	41.0
26	37.04273931456846	39.0	36.0	41.0	31.0	41.0
27	36.8538936971878	39.0	36.0	41.0	30.0	41.0
28	36.665965845084116	39.0	36.0	40.0	30.0	41.0
29	36.49956169134087	39.0	36.0	40.0	30.0	41.0
30	36.36348448752101	39.0	35.0	40.0	29.0	41.0
31	36.20619046169215	39.0	35.0	40.0	29.0	41.0
32	36.06299782624227	39.0	35.0	40.0	28.0	41.0
33	35.898459498802794	38.0	35.0	40.0	27.0	41.0
34	35.774074494987936	38.0	35.0	40.0	27.0	41.0
35	35.61185236052123	38.0	35.0	40.0	26.0	41.0
36	35.52588070950536	38.0	34.0	40.0	26.0	41.0
37	35.44730182434675	38.0	34.0	40.0	25.0	41.0
38	35.33822597132545	38.0	34.0	40.0	25.0	41.0
39	35.21007525102516	38.0	34.0	40.0	25.0	41.0
40	35.1144296094079	38.0	34.0	40.0	24.0	41.0
41	34.91513708299257	38.0	34.0	40.0	24.0	41.0
42	34.73542736752241	38.0	33.0	40.0	23.0	41.0
43	34.46092797841134	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	18.0
11	26.0
12	55.0
13	66.0
14	102.0
15	197.0
16	368.0
17	585.0
18	1183.0
19	2247.0
20	4316.0
21	8203.0
22	14146.0
23	22959.0
24	34411.0
25	48550.0
26	65873.0
27	86304.0
28	113004.0
29	142389.0
30	177475.0
31	221456.0
32	268800.0
33	326924.0
34	402616.0
35	497342.0
36	633878.0
37	833787.0
38	1302735.0
39	1424574.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.72347154927641	20.306186030373524	12.279048273338203	26.691294147011856
2	19.38233145841328	22.367095861479992	33.60585440495681	24.64471827514992
3	19.196954629024777	24.71991503926239	29.656645154172207	26.42648517754063
4	14.711525678888565	16.068714980901618	34.41134453743515	34.80841480277467
5	13.366650619465185	38.56339965942151	34.393996075720686	13.67595364539262
6	34.94245465510022	37.23034144968027	14.504519794278291	13.322684100941217
7	29.228585803441774	31.30636177586752	20.905996659328363	18.559055761362337
8	27.323510677518474	35.665136404729516	18.886536237183467	18.124816680568546
9	27.755956129342657	13.825849780710017	17.67032014317681	40.74787394677052
10	15.8705265160159	27.320616755147338	33.55896381903701	23.249892909799755
11	37.784663236363826	21.72096740207464	20.506062616642403	19.988306744919132
12	21.619077218590917	25.270167247611536	29.59781713847147	23.512938395326074
13	30.564975038412296	19.923208564080937	24.333154372370036	25.178662025136727
14	22.690732846651958	20.26339516781283	24.260173267572966	32.78569871796225
15	25.247437898988245	28.18791926077165	21.13968089079754	25.424961949442576
16	25.292971958796574	26.438106084562218	23.220833105989605	25.048088850651602
17	24.39777023281304	26.54453309426319	24.22598880956393	24.831707863359835
18	24.89633879631519	25.594512640863933	24.8894205131467	24.619728049674176
19	25.544939147745893	25.56323717773838	24.68630333672264	24.20552033779309
20	25.156143691686335	25.032142132585655	24.644446969927625	25.167267205800385
21	25.40140361264005	25.42880544009174	24.694608291027304	24.47518265624091
22	25.44440549037364	24.936627621825842	24.32796942812175	25.29099745967877
23	24.716493578959014	24.94790186106339	24.756933129593158	25.578671430384436
24	24.915089001678172	25.38744646620426	24.52431904650081	25.173145485616754
25	25.375328166275136	24.82986901685318	24.42544336548702	25.369359451384664
26	25.26416838769637	25.283069318182843	24.543536499746633	24.909225794374155
27	25.639489017715327	25.287500636813647	24.081277618494816	24.99173272697621
28	25.044275505027137	25.310124477850493	24.497520119543108	25.14807989757926
29	25.17290432541916	25.474218919801274	24.563899463931026	24.788977290848543
30	25.348484021780383	25.418239608934623	24.488235451935715	24.745040917349275
31	25.517205725022514	24.929860063780843	24.4309900500317	25.12194416116495
32	24.86970566699334	25.290469921746528	24.337329458290892	25.50249495296924
33	24.87190625379639	24.877799606125105	24.687991458105802	25.562302681972703
34	25.35350316839282	24.93665776685054	24.871243063253004	24.838596001503635
35	25.5779931673287	24.973057884174978	24.661267893709848	24.78768105478647
36	25.153807452272137	25.139413202978204	24.73987104561334	24.966908299136314
37	25.293258336531217	24.809144312372393	24.93270876861493	24.96488858248146
38	25.09597422238648	24.556589295441437	25.1574700727731	25.189966409398977
39	25.148170332653365	24.671728217280513	25.000052753793224	25.180048696272898
40	25.390958361581735	24.479840062556953	25.288450205091678	24.840751370769638
41	24.58340329491149	24.801427186049366	25.492803327528406	25.122366191510736
42	25.135388842180852	24.728084340955906	25.436869234198806	24.699657582664443
43	24.66429746869213	24.198285531865253	25.4349550251304	25.702461974312218
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	208.0
1	248.5
2	289.0
3	348.5
4	408.0
5	408.0
6	600.0
7	792.0
8	1029.5
9	1267.0
10	1845.5
11	2424.0
12	2424.0
13	4885.0
14	7346.0
15	11362.0
16	15378.0
17	15149.5
18	14921.0
19	14921.0
20	19315.0
21	23709.0
22	26501.0
23	29293.0
24	37819.0
25	46345.0
26	46345.0
27	58171.0
28	69997.0
29	83362.5
30	96728.0
31	115390.5
32	134053.0
33	134053.0
34	160437.0
35	186821.0
36	221412.0
37	256003.0
38	293334.0
39	330665.0
40	330665.0
41	360037.0
42	389409.0
43	419677.5
44	449946.0
45	486455.0
46	522964.0
47	522964.0
48	563996.5
49	605029.0
50	614115.0
51	623201.0
52	601350.0
53	579499.0
54	579499.0
55	559124.5
56	538750.0
57	505981.0
58	473212.0
59	431291.5
60	389371.0
61	389371.0
62	336102.5
63	282834.0
64	241783.5
65	200733.0
66	167364.0
67	133995.0
68	133995.0
69	109541.5
70	85088.0
71	71963.0
72	58838.0
73	46481.0
74	34124.0
75	34124.0
76	26746.0
77	19368.0
78	16079.0
79	12790.0
80	9977.0
81	7164.0
82	7164.0
83	5820.5
84	4477.0
85	3606.0
86	2735.0
87	2491.5
88	2248.0
89	2248.0
90	1567.5
91	887.0
92	722.5
93	558.0
94	530.5
95	503.0
96	503.0
97	296.0
98	89.0
99	112.0
100	135.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	6634594.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.88823307658534
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.57876629532659	45.592931048134965
2	9.457550105375406	10.571315292454099
3	3.308059591215271	5.5464481639521805
4	1.614500587555212	3.6092634055827864
5	0.9651996828050778	2.697165241902822
6	0.6157692847259186	2.0648554383698623
7	0.4300241766574971	1.6823303989520633
8	0.30271677663648094	1.3534644615081817
9	0.2263754284814179	1.1386550438803222
>10	1.3610703306987506	13.98736021288224
>50	0.09163437626829275	3.485201528081725
>100	0.042129046771736775	4.310073857252702
>500	0.003901444811634362	1.5785284204323833
>1k	0.002275780051141324	2.2789601685745335
>5k	2.7092619556056954E-5	0.10344731803913257
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6832	0.10297540437289757	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.5072512349662993E-5	0.0	0.0	0.0	3.0145024699325986E-5
7	1.5072512349662993E-5	0.0	0.0	0.0	4.5217537048988984E-5
8	1.5072512349662993E-5	0.0	0.0	0.0	6.029004939865197E-5
9	1.5072512349662993E-5	0.0	0.0	0.0	9.043507409797797E-5
10	1.5072512349662993E-5	0.0	0.0	1.5072512349662993E-5	9.043507409797797E-5
11	1.5072512349662993E-5	0.0	0.0	1.5072512349662993E-5	1.0550758644764096E-4
12	1.5072512349662993E-5	0.0	0.0	1.5072512349662993E-5	1.0550758644764096E-4
13	1.5072512349662993E-5	0.0	0.0	1.5072512349662993E-5	1.0550758644764096E-4
14	1.5072512349662993E-5	0.0	0.0	1.5072512349662993E-5	1.8087014819595594E-4
15	1.5072512349662993E-5	1.5072512349662993E-5	0.0	3.0145024699325986E-5	1.959426605456189E-4
16	3.0145024699325986E-5	1.5072512349662993E-5	0.0	4.5217537048988984E-5	2.411601975946079E-4
17	3.0145024699325986E-5	1.5072512349662993E-5	0.0	4.5217537048988984E-5	2.562327099442709E-4
18	3.0145024699325986E-5	1.5072512349662993E-5	0.0	4.5217537048988984E-5	2.7130522229393386E-4
19	3.0145024699325986E-5	3.0145024699325986E-5	0.0	6.029004939865197E-5	2.7130522229393386E-4
20	3.0145024699325986E-5	3.0145024699325986E-5	0.0	7.536256174831497E-5	2.7130522229393386E-4
21	3.0145024699325986E-5	3.0145024699325986E-5	0.0	1.6579763584629293E-4	2.7130522229393386E-4
22	3.0145024699325986E-5	3.0145024699325986E-5	0.0	2.7130522229393386E-4	2.7130522229393386E-4
23	3.0145024699325986E-5	3.0145024699325986E-5	0.0	5.576829569375307E-4	3.4666778404224887E-4
24	3.0145024699325986E-5	3.0145024699325986E-5	0.0	8.742057162804536E-4	3.4666778404224887E-4
25	3.0145024699325986E-5	4.5217537048988984E-5	0.0	0.0010249308397770837	3.768128087415748E-4
26	3.0145024699325986E-5	4.5217537048988984E-5	0.0	0.0014318886732179845	3.918853210912378E-4
27	3.0145024699325986E-5	4.5217537048988984E-5	0.0	0.0042052309455559754	3.918853210912378E-4
28	3.0145024699325986E-5	4.5217537048988984E-5	0.0	0.009450465243238696	3.918853210912378E-4
29	3.0145024699325986E-5	4.5217537048988984E-5	0.0	0.017092229004517834	3.918853210912378E-4
30	3.0145024699325986E-5	4.5217537048988984E-5	0.0	0.03085343277976015	3.918853210912378E-4
31	3.0145024699325986E-5	4.5217537048988984E-5	0.0	0.09878524593969126	3.918853210912378E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAGACG	1665	0.0	16.444445	4
AAGACGG	1650	0.0	15.248486	5
TATACTG	1195	0.0	14.861925	5
TAACGAA	675	0.0	14.251851	13
CGCTTCG	1000	0.0	14.245	32
TATACCG	325	0.0	14.230769	5
GTATTAG	1305	0.0	13.89272	1
CGCAAGA	1795	0.0	13.810585	2
GACGGAC	1760	0.0	13.664773	7
CGATAAC	665	0.0	13.631578	10
ACGGACC	1730	0.0	13.580924	8
AACGCTT	1000	0.0	13.505	30
ATAACGA	700	0.0	13.478572	12
TAGTACT	770	0.0	13.454545	4
TTATACT	1270	0.0	13.401575	4
GCGCAAG	1845	0.0	13.336043	1
GTAAACG	1055	0.0	13.327015	27
TCGTTAC	745	0.0	13.161074	23
CCGATAA	695	0.0	13.043165	9
CGAACGT	385	0.0	12.974026	4
>>END_MODULE