FastQCFastQC Report
Thu 9 Feb 2017
SRR2727254.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727254.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3313225
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC752.0687468E-417.2666663
TAGTACT2700.016.4444454
ATTAGAG4000.015.7249993
TTAGAGT4400.015.1363644
GTATTAC1853.0559022E-1015.01
TAGAGTG5200.014.94230655
AAGACGG7900.014.7531645
AATTAAC4350.014.4597692
CAAGACG8200.014.4390244
TCTTATA9750.014.42051437
CTTAGCG1304.449759E-614.2307683
GCGATTC2101.364242E-1014.09523913
CTAGTAC2650.013.9622643
GTCTTAG3850.013.9350661
CGCTTCG4650.013.92473132
TAGACCG1601.7864295E-713.8750015
AACGCTT4800.013.87500130
GTTAGAC3100.013.7258063
GTAAACG4450.013.71910227
CGAGCCG7150.013.45454615
Produced by FastQC (version 0.11.5)