##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727254.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3313225
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46975077152925	31.0	31.0	34.0	30.0	34.0
2	31.687737476325935	31.0	31.0	34.0	30.0	34.0
3	31.86200212783617	31.0	31.0	34.0	30.0	34.0
4	35.58463128824635	37.0	35.0	37.0	33.0	37.0
5	35.380734178934425	37.0	35.0	37.0	33.0	37.0
6	35.385604358291395	37.0	35.0	37.0	33.0	37.0
7	35.27453583743935	37.0	35.0	37.0	32.0	37.0
8	35.233184586015135	37.0	35.0	37.0	32.0	37.0
9	36.750951716228144	39.0	35.0	39.0	32.0	39.0
10	36.55404688785096	38.0	35.0	39.0	32.0	39.0
11	36.59563627583397	38.0	35.0	39.0	32.0	39.0
12	36.42842577850885	38.0	35.0	39.0	32.0	39.0
13	36.40658210655781	38.0	35.0	39.0	32.0	39.0
14	37.38181952629236	39.0	36.0	41.0	32.0	41.0
15	37.299756883399105	39.0	36.0	41.0	32.0	41.0
16	37.20655282994665	39.0	36.0	40.0	31.0	41.0
17	37.52860581457643	39.0	36.0	41.0	32.0	41.0
18	37.55740917082299	39.0	36.0	41.0	32.0	41.0
19	37.617172090636764	39.0	37.0	41.0	32.0	41.0
20	37.57495974465966	39.0	37.0	41.0	32.0	41.0
21	37.513938534207604	39.0	36.0	41.0	32.0	41.0
22	37.445015958771286	39.0	36.0	41.0	32.0	41.0
23	37.312380837401626	39.0	36.0	41.0	31.0	41.0
24	37.17009892174543	39.0	36.0	41.0	31.0	41.0
25	37.086448098152104	39.0	36.0	41.0	31.0	41.0
26	36.76889435519773	39.0	36.0	41.0	30.0	41.0
27	36.574459929524856	39.0	36.0	40.0	30.0	41.0
28	36.355849663092606	39.0	35.0	40.0	29.0	41.0
29	36.1620819594202	39.0	35.0	40.0	28.0	41.0
30	36.009687540085565	39.0	35.0	40.0	27.0	41.0
31	35.851557319530066	38.0	35.0	40.0	27.0	41.0
32	35.68627877672057	38.0	35.0	40.0	26.0	41.0
33	35.50973417138891	38.0	34.0	40.0	26.0	41.0
34	35.37497604297927	38.0	34.0	40.0	25.0	41.0
35	35.20232401964853	38.0	34.0	40.0	25.0	41.0
36	35.09654188894506	38.0	34.0	40.0	24.0	41.0
37	35.00744531385583	38.0	34.0	40.0	24.0	41.0
38	34.891186985489966	38.0	33.0	40.0	24.0	41.0
39	34.7479857238793	38.0	33.0	40.0	23.0	41.0
40	34.6454134566774	38.0	33.0	40.0	23.0	41.0
41	34.41994220132952	38.0	33.0	40.0	22.0	41.0
42	34.23246172535822	38.0	33.0	40.0	21.0	41.0
43	33.95311788363301	38.0	32.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	7.0
12	21.0
13	21.0
14	40.0
15	83.0
16	172.0
17	354.0
18	673.0
19	1420.0
20	2663.0
21	5042.0
22	8739.0
23	13770.0
24	20697.0
25	28614.0
26	38258.0
27	50140.0
28	63220.0
29	78322.0
30	97311.0
31	118154.0
32	142242.0
33	170624.0
34	207385.0
35	254115.0
36	317617.0
37	412259.0
38	626154.0
39	655107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.0708901447985	19.627704125134876	12.036671219129397	27.264734510937227
2	20.52640554142867	22.016192682356316	31.99891344535913	25.45848833085589
3	19.858264983513045	23.844079409035004	28.938753027639237	27.35890257981272
4	15.486844388775287	15.764881648544845	32.76955232439692	35.97872163828294
5	14.067954938164476	38.12687034535838	33.493922084977626	14.31125263149952
6	36.477480400516114	35.789962951504954	13.92024387115273	13.812312776826206
7	30.15575458956153	30.620196334387188	20.054388096190266	19.16966097986101
8	28.21906148842895	34.33062952259505	18.48326026756408	18.967048721411917
9	28.178134596956138	13.447924605180752	16.92716311146994	41.44677768639317
10	16.69940918591403	26.60066853292487	32.69219567038157	24.007726610779528
11	38.7743361830241	21.020576628511495	19.584875763040543	20.62021142542387
12	22.275396328350777	24.619064506636285	28.446755049838146	24.658784115174793
13	31.400342566532608	19.086056636660658	23.433090116125527	26.080510680681208
14	23.486874570848645	19.827086901734713	22.891231353137805	33.79480717427884
15	26.273313765289107	27.314414203683725	20.246346082744154	26.165925948283018
16	26.443631205245644	25.31467075130726	22.416014608123504	25.825683435323587
17	25.532072225701548	25.558662632329526	23.027805235080624	25.881459906888303
18	25.884991209471135	24.50733047106671	23.905137743437287	25.70254057602487
19	26.465483026356495	24.703453583743933	23.52505489364592	25.30600849625365
20	26.051294433671117	23.895388933742804	23.61016834051415	26.44314829207192
21	26.338386315447938	24.5646160462993	23.726701325747573	25.370296312505186
22	26.552618672139683	23.904021006723056	23.17283009756355	26.370530223573706
23	25.749775520829406	23.961216035735575	23.60114390058025	26.687864542854772
24	25.854326222939882	24.455930400138836	23.396720717729703	26.29302265919157
25	26.50330116427348	23.709980457107502	23.140686189437783	26.646032189181234
26	26.314723569935637	24.36574636494654	23.38199186593123	25.937538199186594
27	26.887700050554976	24.159150072814253	22.96068030393348	25.992469572697296
28	26.15738442152284	24.48396954628798	23.228878207788483	26.129767824400695
29	26.13378204015725	24.487380120577384	23.532781504425447	25.846056334839922
30	26.367994929411676	24.53361905696112	23.362916795569273	25.735469218057933
31	26.681979038550054	23.94223151159369	23.19489319318791	26.18089625666835
32	25.96545661704231	24.233458337420487	23.091730866451872	26.70935417908533
33	25.893351643791167	23.817247545820162	23.463302371556413	26.826098438832258
34	26.568373714432315	24.009114986153975	23.775535920440056	25.646975378973657
35	26.670208029940618	23.90335700110919	23.714326675670986	25.712108293279208
36	26.11657825834346	24.125255604433747	23.749488791132507	26.00867734609029
37	26.346414746961045	23.822891593538017	23.92535973258683	25.90533392691411
38	26.111869854899684	23.467678772193256	24.028884244203155	26.391567128703908
39	26.234891985904973	23.576153143840216	23.959706932067697	26.22924793818711
40	26.46210263414045	23.366900829252465	24.222381516498277	25.948615020108807
41	25.479283779399225	23.81703627130666	24.60934587901516	26.094334070278958
42	26.14292720838458	23.604856295603227	24.651902602449276	25.60031389356292
43	25.58685268884546	22.864037305042668	24.538538734918394	27.010571271193474
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	43.0
1	51.5
2	60.0
3	74.5
4	89.0
5	89.0
6	122.0
7	155.0
8	189.0
9	223.0
10	300.0
11	377.0
12	377.0
13	763.0
14	1149.0
15	1808.0
16	2467.0
17	2455.5
18	2444.0
19	2444.0
20	3317.0
21	4190.0
22	5021.0
23	5852.0
24	7965.5
25	10079.0
26	10079.0
27	13322.5
28	16566.0
29	20809.5
30	25053.0
31	32126.0
32	39199.0
33	39199.0
34	49777.5
35	60356.0
36	76723.5
37	93091.0
38	113390.0
39	133689.0
40	133689.0
41	151846.5
42	170004.0
43	189372.0
44	208740.0
45	234854.0
46	260968.0
47	260968.0
48	290963.5
49	320959.0
50	329072.5
51	337186.0
52	328974.5
53	320763.0
54	320763.0
55	310409.0
56	300055.0
57	284512.5
58	268970.0
59	247634.5
60	226299.0
61	226299.0
62	196608.5
63	166918.0
64	144544.0
65	122170.0
66	102396.0
67	82622.0
68	82622.0
69	66552.0
70	50482.0
71	42359.0
72	34236.0
73	27043.5
74	19851.0
75	19851.0
76	15312.0
77	10773.0
78	8996.0
79	7219.0
80	5512.0
81	3805.0
82	3805.0
83	3112.0
84	2419.0
85	1953.5
86	1488.0
87	1324.5
88	1161.0
89	1161.0
90	794.5
91	428.0
92	361.0
93	294.0
94	268.5
95	243.0
96	243.0
97	138.0
98	33.0
99	45.0
100	57.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3313225.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.31669653166863
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.60884530074495	47.34866116934358
2	9.00611838528429	10.324019088352424
3	3.0875092451492394	5.308974913288207
4	1.4533765729751358	3.3321097591780964
5	0.8719969182919254	2.498999137114427
6	0.5751086588643022	1.9777997083716088
7	0.40546669860605805	1.6268008202390685
8	0.30713718347360086	1.408327099099825
9	0.23495806093500565	1.2120317888652925
>10	1.3329447600573174	13.748658165525592
>50	0.06830597788288438	2.683124571643389
>100	0.04084654292806919	4.63670257591947
>500	0.005328251255712802	2.0428691164367385
>1k	0.002057443551499653	1.8509220866223597
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.036414671505859E-5
2	0.0	0.0	0.0	0.0	6.036414671505859E-5
3	0.0	0.0	0.0	0.0	6.036414671505859E-5
4	0.0	0.0	0.0	0.0	6.036414671505859E-5
5	0.0	0.0	0.0	0.0	6.036414671505859E-5
6	0.0	0.0	0.0	0.0	9.054622007258789E-5
7	0.0	0.0	0.0	0.0	9.054622007258789E-5
8	0.0	0.0	0.0	0.0	9.054622007258789E-5
9	0.0	0.0	0.0	0.0	2.1127451350270508E-4
10	0.0	0.0	0.0	0.0	2.1127451350270508E-4
11	0.0	0.0	0.0	0.0	2.1127451350270508E-4
12	0.0	0.0	0.0	0.0	2.1127451350270508E-4
13	0.0	3.0182073357529297E-5	0.0	0.0	2.4145658686023437E-4
14	0.0	3.0182073357529297E-5	0.0	0.0	3.3200280693282223E-4
15	0.0	3.0182073357529297E-5	0.0	0.0	3.6218488029035156E-4
16	3.0182073357529297E-5	3.0182073357529297E-5	0.0	3.0182073357529297E-5	5.13095247077998E-4
17	3.0182073357529297E-5	3.0182073357529297E-5	0.0	3.0182073357529297E-5	5.432773204355273E-4
18	3.0182073357529297E-5	3.0182073357529297E-5	0.0	3.0182073357529297E-5	6.036414671505859E-4
19	3.0182073357529297E-5	3.0182073357529297E-5	0.0	3.0182073357529297E-5	6.036414671505859E-4
20	3.0182073357529297E-5	3.0182073357529297E-5	0.0	6.036414671505859E-5	6.036414671505859E-4
21	3.0182073357529297E-5	3.0182073357529297E-5	0.0	1.5091036678764648E-4	6.338235405081152E-4
22	3.0182073357529297E-5	3.0182073357529297E-5	0.0	3.0182073357529297E-4	6.338235405081152E-4
23	3.0182073357529297E-5	3.0182073357529297E-5	0.0	4.527311003629394E-4	7.847339072957617E-4
24	3.0182073357529297E-5	3.0182073357529297E-5	0.0	6.338235405081152E-4	7.847339072957617E-4
25	3.0182073357529297E-5	3.0182073357529297E-5	0.0	8.149159806532909E-4	7.847339072957617E-4
26	3.0182073357529297E-5	3.0182073357529297E-5	0.0	0.0014487395211614062	8.450980540108203E-4
27	3.0182073357529297E-5	3.0182073357529297E-5	0.0	0.003923669536478809	8.450980540108203E-4
28	3.0182073357529297E-5	3.0182073357529297E-5	0.0	0.008330252246678086	8.450980540108203E-4
29	3.0182073357529297E-5	3.0182073357529297E-5	0.0	0.01578522436598782	8.450980540108203E-4
30	3.0182073357529297E-5	3.0182073357529297E-5	0.0	0.029065336643300712	8.450980540108203E-4
31	3.0182073357529297E-5	3.0182073357529297E-5	0.0	0.09296078594119023	8.450980540108203E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAC	75	2.0687468E-4	17.266666	3
TAGTACT	270	0.0	16.444445	4
ATTAGAG	400	0.0	15.724999	3
TTAGAGT	440	0.0	15.136364	4
GTATTAC	185	3.0559022E-10	15.0	1
TAGAGTG	520	0.0	14.9423065	5
AAGACGG	790	0.0	14.753164	5
AATTAAC	435	0.0	14.459769	2
CAAGACG	820	0.0	14.439024	4
TCTTATA	975	0.0	14.420514	37
CTTAGCG	130	4.449759E-6	14.230768	3
GCGATTC	210	1.364242E-10	14.095239	13
CTAGTAC	265	0.0	13.962264	3
GTCTTAG	385	0.0	13.935066	1
CGCTTCG	465	0.0	13.924731	32
TAGACCG	160	1.7864295E-7	13.875001	5
AACGCTT	480	0.0	13.875001	30
GTTAGAC	310	0.0	13.725806	3
GTAAACG	445	0.0	13.719102	27
CGAGCCG	715	0.0	13.454546	15
>>END_MODULE