FastQCFastQC Report
Thu 9 Feb 2017
SRR2727253.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727253.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4032814
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT44700.11084071816850466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG11850.018.2658235
CAAGACG12200.017.5901624
CGCTATA650.001580863517.07692314
TTAGAGT6200.017.0080644
ACGGACC12250.016.0081638
GACGGAC12900.015.7751937
CGGACCA13250.015.0792459
TTATCTA7400.014.754
GCGCAAG14400.014.5173611
AGACGGA14400.014.5173616
TAAACGC6400.014.45312528
GTATTAG9350.014.443851
TAGAGTG6150.014.4390255
ATTAGAG5900.014.4237293
TATACCG1803.3378456E-914.3888885
AGGTTAT7700.014.1753241
CGCTTCG6300.014.09523832
CGAACGA6700.014.08208916
GCGAAAG14300.013.97202718
CGCAAGA14600.013.9383572
Produced by FastQC (version 0.11.5)