##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727253.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4032814
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.474424310171507	31.0	31.0	34.0	30.0	34.0
2	31.68488851705038	31.0	31.0	34.0	30.0	34.0
3	31.858474008471504	31.0	31.0	34.0	30.0	34.0
4	35.57889577848123	37.0	35.0	37.0	33.0	37.0
5	35.37218155858415	37.0	35.0	37.0	33.0	37.0
6	35.383270589717256	37.0	35.0	37.0	33.0	37.0
7	35.27623639473578	37.0	35.0	37.0	32.0	37.0
8	35.23895746245674	37.0	35.0	37.0	32.0	37.0
9	36.762473300281144	39.0	35.0	39.0	32.0	39.0
10	36.54748867664117	38.0	35.0	39.0	32.0	39.0
11	36.61254573109496	38.0	35.0	39.0	32.0	39.0
12	36.43463001269089	38.0	35.0	39.0	32.0	39.0
13	36.4270544091545	38.0	35.0	39.0	32.0	39.0
14	37.387264327092694	39.0	36.0	41.0	32.0	41.0
15	37.31279573022708	39.0	36.0	41.0	32.0	41.0
16	37.20987404824522	39.0	36.0	40.0	31.0	41.0
17	37.538835165718034	39.0	36.0	41.0	32.0	41.0
18	37.56451152966638	39.0	36.0	41.0	32.0	41.0
19	37.632859586383105	39.0	37.0	41.0	32.0	41.0
20	37.59055413911973	39.0	37.0	41.0	32.0	41.0
21	37.535844698019794	39.0	37.0	41.0	32.0	41.0
22	37.4698798407266	39.0	36.0	41.0	32.0	41.0
23	37.335133730442315	39.0	36.0	41.0	31.0	41.0
24	37.19758684630633	39.0	36.0	41.0	31.0	41.0
25	37.105149158875165	39.0	36.0	41.0	31.0	41.0
26	36.79306186697428	39.0	36.0	41.0	30.0	41.0
27	36.59353419225385	39.0	36.0	40.0	30.0	41.0
28	36.3604505439626	39.0	35.0	40.0	29.0	41.0
29	36.16741610200718	39.0	35.0	40.0	28.0	41.0
30	36.02321902274689	39.0	35.0	40.0	27.0	41.0
31	35.85171272466322	38.0	35.0	40.0	27.0	41.0
32	35.68451582443426	38.0	35.0	40.0	26.0	41.0
33	35.505420532660324	38.0	34.0	40.0	26.0	41.0
34	35.36250543665044	38.0	34.0	40.0	25.0	41.0
35	35.18092503150405	38.0	34.0	40.0	25.0	41.0
36	35.06837235736634	38.0	34.0	40.0	24.0	41.0
37	34.98245865046094	38.0	34.0	40.0	24.0	41.0
38	34.853356490034	38.0	33.0	40.0	24.0	41.0
39	34.691557061644794	38.0	33.0	40.0	23.0	41.0
40	34.59339260377493	38.0	33.0	40.0	23.0	41.0
41	34.359968498423186	38.0	33.0	40.0	22.0	41.0
42	34.17779148753203	38.0	33.0	40.0	21.0	41.0
43	33.89402784259329	38.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	12.0
11	16.0
12	30.0
13	47.0
14	71.0
15	132.0
16	242.0
17	517.0
18	949.0
19	1783.0
20	3521.0
21	6186.0
22	10737.0
23	17047.0
24	25118.0
25	34482.0
26	46135.0
27	60512.0
28	77701.0
29	95232.0
30	117612.0
31	143332.0
32	172751.0
33	208092.0
34	252746.0
35	309251.0
36	388068.0
37	502496.0
38	766639.0
39	791354.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.02988632751226	19.822064692296742	12.175319764313453	26.97272921587755
2	20.227017660621094	22.307351640814577	32.42557182156182	25.040058877002508
3	19.63192450730433	24.34287819869699	29.371401706103978	26.653795587894706
4	15.19264216004011	15.877325361397773	33.50040443224012	35.429628046322
5	13.763813555497476	38.6400910133718	33.62128776581315	13.974807665317568
6	35.774002966662984	36.38637933710803	14.262671176999483	13.576946519229502
7	29.646643757931805	31.217755145662558	20.4026270490035	18.732974047402138
8	27.91118062970422	34.752879750962975	18.794519162054087	18.541420457278715
9	28.422039796529177	13.549620686696684	17.113137377523486	40.91520213925065
10	16.477948152332342	26.885594029379984	32.74200099483884	23.894456823448838
11	38.51298373790609	21.329597645713392	19.7674626203936	20.389955995986924
12	22.091869349789008	25.000334753846822	28.523557000149275	24.384238896214903
13	31.022531661514762	19.266943627948127	23.694546785445596	26.01597792509151
14	23.226635297338287	20.14407309635406	23.232536883674772	33.396754722632885
15	26.14283723474477	27.715337231025284	20.419463927669366	25.72236160656058
16	26.03849322086265	25.681422450923847	22.798224763155453	25.481859565058045
17	25.119531919895138	25.784848991299867	23.27503822392999	25.820580864874998
18	25.646831220086025	24.892395235684063	24.175848427425613	25.284925116804295
19	26.406201724155885	24.909132928024945	23.720434416266162	24.964230931553004
20	25.691489862909624	24.162061528252977	23.81862887799933	26.32781973083807
21	26.202522605803292	24.902785003226036	23.99825531254355	24.896437078427123
22	26.287029354688812	24.130371497420906	23.40556742760762	26.177031720282663
23	25.23466244661916	24.144753514543442	24.153035572679524	26.467548466157876
24	25.47355271034072	24.61888894454344	23.824976802798243	26.082581542317595
25	26.150821733905904	23.891605216605576	23.47403078842714	26.483542261061384
26	26.025400625964895	24.660819963430004	23.707688973505846	25.606090437099255
27	26.780530914641737	24.40782044497961	23.190333102394504	25.621315537984145
28	25.672743647487835	25.04705151291381	23.35808197452201	25.922122865076346
29	25.820655254618735	24.95894925974766	23.82510078570447	25.39529469992913
30	26.34981429840305	24.76446967303724	23.541899031296758	25.34381699726295
31	26.53432565945268	24.28108511823258	23.40016177289605	25.784427449418697
32	25.649434861116827	24.60225043852754	23.259862716207593	26.488451984148043
33	25.531700693362	23.949108488514472	23.79933713779014	26.719853680333383
34	26.392042876264565	24.301244738785375	24.07328976739319	25.233422617556872
35	26.557634445823687	24.140786061544123	23.877074419995566	25.424505072636627
36	25.883489791495467	24.454710780115324	24.036590827149478	25.62520860123973
37	26.09453349447805	24.24503088910126	24.288276126793846	25.372159489626846
38	26.16961754249018	23.566422850148804	24.241013842939445	26.022945764421568
39	26.043675706343016	23.91848471067597	24.14857218805529	25.889267394925724
40	26.524704585929328	23.53961774582215	24.42455813732049	25.511119530928035
41	25.23215799191334	24.303823583234934	24.689807166906284	25.774211257945446
42	26.240783730665484	24.00990970572905	24.716141136189272	25.033165427416193
43	25.42090956835599	23.326987061639837	24.492004838308933	26.760098531695238
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	63.0
1	73.0
2	83.0
3	103.5
4	124.0
5	124.0
6	182.0
7	240.0
8	282.5
9	325.0
10	491.0
11	657.0
12	657.0
13	1341.0
14	2025.0
15	3131.0
16	4237.0
17	4197.0
18	4157.0
19	4157.0
20	5530.5
21	6904.0
22	8264.5
23	9625.0
24	12775.5
25	15926.0
26	15926.0
27	20806.0
28	25686.0
29	31736.5
30	37787.0
31	47275.5
32	56764.0
33	56764.0
34	71191.0
35	85618.0
36	106733.5
37	127849.0
38	153413.5
39	178978.0
40	178978.0
41	198630.0
42	218282.0
43	238722.5
44	259163.0
45	288869.5
46	318576.0
47	318576.0
48	356497.5
49	394419.0
50	400217.5
51	406016.0
52	392143.0
53	378270.0
54	378270.0
55	363207.0
56	348144.0
57	329873.5
58	311603.0
59	286427.5
60	261252.0
61	261252.0
62	224920.5
63	188589.0
64	163036.5
65	137484.0
66	115609.5
67	93735.0
68	93735.0
69	76135.5
70	58536.0
71	49947.0
72	41358.0
73	32852.5
74	24347.0
75	24347.0
76	18802.0
77	13257.0
78	11183.5
79	9110.0
80	6970.5
81	4831.0
82	4831.0
83	3994.0
84	3157.0
85	2640.5
86	2124.0
87	1950.0
88	1776.0
89	1776.0
90	1221.5
91	667.0
92	567.5
93	468.0
94	446.5
95	425.0
96	425.0
97	247.5
98	70.0
99	88.5
100	107.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4032814.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.87566191864454
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.35934357734779	45.195234939920255
2	9.01170167261233	9.890461885959153
3	3.2120356337371536	5.287877445227975
4	1.4973805178147246	3.286789882366628
5	0.9077035977927941	2.490541787740733
6	0.5877800725617307	1.9352892326648334
7	0.403397446256931	1.5495691315747946
8	0.30175424912634535	1.3247171326057412
9	0.23513245939554192	1.1612744413100253
>10	1.3513477783217764	13.47201791862758
>50	0.07540263092574957	2.8278735908106976
>100	0.04602602984130035	5.122019494537429
>500	0.007284312051584901	2.900163348105683
>1k	0.0037100222144740625	3.556169768548586
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	4470	0.11084071816850466	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.4796581245750486E-5
2	0.0	0.0	0.0	0.0	2.4796581245750486E-5
3	0.0	0.0	0.0	0.0	2.4796581245750486E-5
4	0.0	0.0	0.0	2.4796581245750486E-5	4.959316249150097E-5
5	0.0	0.0	0.0	2.4796581245750486E-5	4.959316249150097E-5
6	0.0	0.0	0.0	2.4796581245750486E-5	1.2398290622875243E-4
7	0.0	0.0	0.0	2.4796581245750486E-5	1.2398290622875243E-4
8	0.0	0.0	0.0	2.4796581245750486E-5	1.487794874745029E-4
9	0.0	0.0	0.0	4.959316249150097E-5	3.223555561947563E-4
10	0.0	0.0	0.0	7.438974373725145E-5	3.223555561947563E-4
11	0.0	0.0	0.0	7.438974373725145E-5	3.471521374405068E-4
12	0.0	0.0	0.0	9.918632498300194E-5	3.471521374405068E-4
13	0.0	0.0	0.0	9.918632498300194E-5	3.471521374405068E-4
14	0.0	0.0	0.0	9.918632498300194E-5	3.967452999320078E-4
15	0.0	0.0	0.0	1.2398290622875243E-4	3.967452999320078E-4
16	0.0	0.0	0.0	1.2398290622875243E-4	5.207282061607602E-4
17	0.0	0.0	0.0	1.487794874745029E-4	5.207282061607602E-4
18	0.0	0.0	0.0	1.487794874745029E-4	5.703213686522612E-4
19	0.0	0.0	0.0	2.2316923121175436E-4	5.703213686522612E-4
20	0.0	0.0	0.0	2.7276239370325534E-4	5.703213686522612E-4
21	0.0	0.0	0.0	3.471521374405068E-4	6.199145311437622E-4
22	0.0	0.0	0.0	4.7113504366925926E-4	6.199145311437622E-4
23	0.0	0.0	0.0	7.934905998640156E-4	7.191008561267641E-4
24	0.0	0.0	0.0	0.0010910495748130214	7.438974373725146E-4
25	0.0	0.0	0.0	0.0012398290622875243	8.182871811097661E-4
26	0.0	0.0	0.0	0.0017109641059567834	8.182871811097661E-4
27	0.0	0.0	0.0	0.003620300861879571	8.182871811097661E-4
28	0.0	0.0	0.0	0.007538160698708148	8.182871811097661E-4
29	0.0	0.0	0.0	0.01428283079755228	8.182871811097661E-4
30	0.0	0.0	0.0	0.024945360733224987	8.182871811097661E-4
31	0.0	0.0	0.0	0.07969621212384206	8.430837623555165E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	1185	0.0	18.265823	5
CAAGACG	1220	0.0	17.590162	4
CGCTATA	65	0.0015808635	17.076923	14
TTAGAGT	620	0.0	17.008064	4
ACGGACC	1225	0.0	16.008163	8
GACGGAC	1290	0.0	15.775193	7
CGGACCA	1325	0.0	15.079245	9
TTATCTA	740	0.0	14.75	4
GCGCAAG	1440	0.0	14.517361	1
AGACGGA	1440	0.0	14.517361	6
TAAACGC	640	0.0	14.453125	28
GTATTAG	935	0.0	14.44385	1
TAGAGTG	615	0.0	14.439025	5
ATTAGAG	590	0.0	14.423729	3
TATACCG	180	3.3378456E-9	14.388888	5
AGGTTAT	770	0.0	14.175324	1
CGCTTCG	630	0.0	14.095238	32
CGAACGA	670	0.0	14.082089	16
GCGAAAG	1430	0.0	13.972027	18
CGCAAGA	1460	0.0	13.938357	2
>>END_MODULE