FastQCFastQC Report
Thu 9 Feb 2017
SRR2727252.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727252.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5027532
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT58070.11550398883587415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAGT8000.018.03754
TAGAGTG8250.017.7151535
ATACCGT13300.016.6917296
CGTCGTA12250.016.00816510
ACCGTCG12300.015.94308958
GGTTATC8100.015.7592592
TACCGTC13200.015.6969697
CCGTCGT12550.015.6254999
TCGTTAC10200.015.41666723
AAGACGG14800.015.3755
ATTAGAG8400.015.1964283
CGCTTCG8350.015.06586832
TATACCG2350.014.9574475
CGAACGT3600.014.9027784
GTCGTAG13350.014.82771611
CGTTACG9300.014.72042924
AACGAAC7600.014.60526414
CGAGCCG12100.014.52479315
CCGATAA7800.014.4679489
CAAGACG15050.014.3820614
Produced by FastQC (version 0.11.5)