##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727252.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5027532
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.495022408609234	31.0	31.0	34.0	30.0	34.0
2	31.705913159777005	31.0	31.0	34.0	30.0	34.0
3	31.881224823631158	31.0	31.0	34.0	30.0	34.0
4	35.59658794812246	37.0	35.0	37.0	33.0	37.0
5	35.3955350259332	37.0	35.0	37.0	33.0	37.0
6	35.40290902176257	37.0	35.0	37.0	33.0	37.0
7	35.30000982589469	37.0	35.0	37.0	32.0	37.0
8	35.26069868874032	37.0	35.0	37.0	32.0	37.0
9	36.78944042524245	39.0	37.0	39.0	32.0	39.0
10	36.58728835539983	38.0	35.0	39.0	32.0	39.0
11	36.64511334786133	39.0	35.0	39.0	32.0	39.0
12	36.4713877504907	38.0	35.0	39.0	32.0	39.0
13	36.45987753036679	38.0	35.0	39.0	32.0	39.0
14	37.43560040990291	39.0	36.0	41.0	32.0	41.0
15	37.36181669256406	39.0	36.0	41.0	32.0	41.0
16	37.268258461607005	39.0	36.0	41.0	31.0	41.0
17	37.58589880681018	39.0	36.0	41.0	32.0	41.0
18	37.613448706045034	39.0	36.0	41.0	32.0	41.0
19	37.68979730014647	40.0	37.0	41.0	32.0	41.0
20	37.64107120551396	40.0	37.0	41.0	32.0	41.0
21	37.590260390187474	39.0	37.0	41.0	32.0	41.0
22	37.525619528627566	39.0	37.0	41.0	32.0	41.0
23	37.391206261839805	39.0	36.0	41.0	31.0	41.0
24	37.25246124738739	39.0	36.0	41.0	31.0	41.0
25	37.15993214961138	39.0	36.0	41.0	31.0	41.0
26	36.85984077276883	39.0	36.0	41.0	30.0	41.0
27	36.66502610028141	39.0	36.0	41.0	30.0	41.0
28	36.432002620769	39.0	36.0	40.0	30.0	41.0
29	36.24355290030974	39.0	35.0	40.0	29.0	41.0
30	36.099812194134216	39.0	35.0	40.0	27.0	41.0
31	35.93535695048784	39.0	35.0	40.0	27.0	41.0
32	35.76941787739989	38.0	35.0	40.0	27.0	41.0
33	35.589940153538556	38.0	34.0	40.0	26.0	41.0
34	35.45346941600769	38.0	34.0	40.0	25.0	41.0
35	35.27786178188423	38.0	34.0	40.0	25.0	41.0
36	35.161989023640224	38.0	34.0	40.0	25.0	41.0
37	35.08395391615608	38.0	34.0	40.0	24.0	41.0
38	34.95678495930011	38.0	34.0	40.0	24.0	41.0
39	34.80185765103037	38.0	33.0	40.0	24.0	41.0
40	34.70191020166555	38.0	33.0	40.0	23.0	41.0
41	34.46519703902432	38.0	33.0	40.0	22.0	41.0
42	34.2850223529159	38.0	33.0	40.0	21.0	41.0
43	34.00674645134034	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	14.0
11	36.0
12	46.0
13	60.0
14	90.0
15	152.0
16	325.0
17	624.0
18	1108.0
19	2203.0
20	4121.0
21	7373.0
22	13036.0
23	20690.0
24	30441.0
25	42071.0
26	56013.0
27	72962.0
28	93054.0
29	116558.0
30	142918.0
31	175437.0
32	211610.0
33	255350.0
34	311518.0
35	382450.0
36	480940.0
37	626260.0
38	963863.0
39	1016206.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.94887909216689	19.97487037377385	12.213408089694903	26.862842444364354
2	20.015307709627706	22.25167338566915	32.94823384515504	24.784785059548103
3	19.426708770824334	24.49500072799139	29.55153741438145	26.526753086802827
4	14.917140258878511	15.869874125117454	33.98375186871013	35.229233747293904
5	13.639813729678895	38.712453744700184	33.810346706893164	13.837385818727757
6	35.46835703880154	36.874812532272294	14.32712909634389	13.32970133258227
7	29.562596518530366	31.305897207615985	20.606989672069716	18.524516601783937
8	27.667074023596467	35.234246147016066	18.801392015008556	18.297287814378905
9	28.31295753065321	13.634960453757431	17.19995019425038	40.85213182133898
10	16.225316914939576	26.952309801310065	33.00651293716281	23.81586034658755
11	38.48033190042351	21.40865538001548	20.018748761817925	20.092263957743086
12	21.985280252815894	24.94006999855993	28.87160141397409	24.20304833465008
13	30.681197056527935	19.322144543286846	24.091144521805134	25.905513878380088
14	23.005263815327282	20.151816040156483	23.427001558617626	33.41591858589861
15	25.701576837303076	28.04208904090516	20.595214510817634	25.661119610974133
16	25.87289349923581	25.96230118475626	22.784658556126544	25.380146759881388
17	24.962844592535664	25.998104039914615	23.40518170744612	25.633869660103603
18	25.4555515509399	25.16879057159656	24.34464862680138	25.031009250662155
19	26.30191115640835	24.97424183476107	23.77054984433714	24.95329716449343
20	25.426511457311463	24.377408239271276	23.865208615280817	26.330871688136448
21	26.05610466527115	24.99743412871365	24.15324258502979	24.793218620985407
22	26.050595003671784	24.213252148370216	23.5984176729258	26.1377351750322
23	25.048751554440628	24.251740217665446	24.440819073851745	26.25868915404218
24	25.293702755149045	24.599783750754845	24.067256061224473	26.039257432871636
25	25.9661997178735	24.069304780158536	23.68792481082169	26.276570691146272
26	25.784599680320287	24.797972444531432	23.978226294730696	25.439201580417585
27	26.55396325672318	24.568456252491284	23.359095476667278	25.51848501411826
28	25.555302283506105	25.216826068934022	23.511814544392756	25.716057103167124
29	25.61726111340515	25.09971095161602	23.99714213653936	25.285885798439473
30	26.16908256377085	24.819672952852414	23.696517496059695	25.314726987317037
31	26.373715771475947	24.449769787641333	23.630719804468676	25.545794636414048
32	25.644093364298826	24.68155349384151	23.338906644452983	26.33544649740668
33	25.441091175550945	24.007982445462307	23.88474106181721	26.66618531716954
34	26.2841290716797	24.46872541040017	24.194694335113134	25.05245118280699
35	26.547001590442388	24.170229050754923	23.979598737511765	25.30317062129092
36	25.71174087007303	24.61521875942311	24.178762064567668	25.49427830593619
37	25.977974879125583	24.456015396818955	24.42767146981859	25.13833825423687
38	26.100579767567865	23.64432488942885	24.429799750653004	25.825295592350283
39	25.933340653028164	24.09438666924447	24.26822942151338	25.704043256213982
40	26.465331299731158	23.724861423060062	24.483901842892298	25.325905434316482
41	25.126463640609348	24.517994117193087	24.729469648328443	25.62607259386912
42	26.156670907315956	24.237319623226664	24.785660240451975	24.820349229005405
43	25.315124796818793	23.56502156525309	24.579415904264756	26.540437733663357
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	95.0
1	110.5
2	126.0
3	150.5
4	175.0
5	175.0
6	224.5
7	274.0
8	372.5
9	471.0
10	667.0
11	863.0
12	863.0
13	1701.0
14	2539.0
15	3815.5
16	5092.0
17	5483.5
18	5875.0
19	5875.0
20	8121.5
21	10368.0
22	12268.5
23	14169.0
24	18803.5
25	23438.0
26	23438.0
27	30574.0
28	37710.0
29	46060.0
30	54410.0
31	66835.0
32	79260.0
33	79260.0
34	97830.5
35	116401.0
36	143690.5
37	170980.0
38	202442.0
39	233904.0
40	233904.0
41	256226.0
42	278548.0
43	302182.0
44	325816.0
45	360436.0
46	395056.0
47	395056.0
48	436136.5
49	477217.0
50	485801.0
51	494385.0
52	479727.0
53	465069.0
54	465069.0
55	445509.0
56	425949.0
57	403127.5
58	380306.0
59	351656.5
60	323007.0
61	323007.0
62	276178.5
63	229350.0
64	198794.5
65	168239.0
66	141210.5
67	114182.0
68	114182.0
69	92255.5
70	70329.0
71	60582.0
72	50835.0
73	40288.0
74	29741.0
75	29741.0
76	22761.5
77	15782.0
78	13385.0
79	10988.0
80	8371.0
81	5754.0
82	5754.0
83	4867.5
84	3981.0
85	3223.0
86	2465.0
87	2313.0
88	2161.0
89	2161.0
90	1498.5
91	836.0
92	730.0
93	624.0
94	576.5
95	529.0
96	529.0
97	315.0
98	101.0
99	116.5
100	132.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5027532.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.1375714752751
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.84759470118085	43.49182413513346
2	9.339312095953717	9.92536728057284
3	3.1814009948555175	5.0715576826693916
4	1.526343263931803	3.2442469713192352
5	0.9413008179498351	2.5009219746772136
6	0.5931751509406064	1.8911932188278142
7	0.42363741242863523	1.5757744297769043
8	0.31911160619296935	1.3565452626135
9	0.2456653674845243	1.1748654921337771
>10	1.433504130281261	14.030151463703197
>50	0.09019050227077251	3.2710665378342467
>100	0.04673250185139506	4.921408604071012
>500	0.006521725108058324	2.4207414291586185
>1k	0.005472248416361538	5.008679967933794
>5k	3.748115353664058E-5	0.11565554957493397
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	5807	0.11550398883587415	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.956190035190228E-5
2	0.0	0.0	0.0	0.0	7.956190035190228E-5
3	0.0	0.0	0.0	0.0	7.956190035190228E-5
4	0.0	0.0	0.0	0.0	7.956190035190228E-5
5	0.0	1.989047508797557E-5	0.0	0.0	7.956190035190228E-5
6	0.0	1.989047508797557E-5	0.0	0.0	7.956190035190228E-5
7	0.0	1.989047508797557E-5	0.0	0.0	7.956190035190228E-5
8	0.0	1.989047508797557E-5	0.0	0.0	1.1934285052785342E-4
9	0.0	1.989047508797557E-5	0.0	0.0	1.9890475087975572E-4
10	0.0	1.989047508797557E-5	0.0	1.989047508797557E-5	1.9890475087975572E-4
11	0.0	1.989047508797557E-5	0.0	1.989047508797557E-5	1.9890475087975572E-4
12	0.0	1.989047508797557E-5	0.0	1.989047508797557E-5	1.9890475087975572E-4
13	0.0	1.989047508797557E-5	0.0	1.989047508797557E-5	1.9890475087975572E-4
14	0.0	1.989047508797557E-5	0.0	1.989047508797557E-5	3.381380764955847E-4
15	0.0	1.989047508797557E-5	0.0	3.978095017595114E-5	3.381380764955847E-4
16	0.0	1.989047508797557E-5	0.0	3.978095017595114E-5	4.17699976847487E-4
17	0.0	1.989047508797557E-5	0.0	5.967142526392671E-5	4.17699976847487E-4
18	0.0	1.989047508797557E-5	0.0	9.945237543987786E-5	4.375904519354626E-4
19	0.0	1.989047508797557E-5	0.0	1.1934285052785342E-4	4.375904519354626E-4
20	0.0	1.989047508797557E-5	0.0	1.39233325615829E-4	4.375904519354626E-4
21	0.0	1.989047508797557E-5	0.0	1.9890475087975572E-4	4.574809270234381E-4
22	0.0	1.989047508797557E-5	0.0	2.3868570105570685E-4	4.574809270234381E-4
23	0.0	3.978095017595114E-5	0.0	3.580285515835603E-4	6.96166628079145E-4
24	0.0	3.978095017595114E-5	0.0	4.972618771993893E-4	6.96166628079145E-4
25	0.0	3.978095017595114E-5	0.0	5.768237775512915E-4	7.160571031671205E-4
26	0.0	3.978095017595114E-5	0.0	8.552904287829496E-4	7.359475782550962E-4
27	0.0	3.978095017595114E-5	0.0	0.0024863093859969466	7.558380533430717E-4
28	0.0	3.978095017595114E-5	0.0	0.005947252051304696	7.558380533430717E-4
29	0.0	3.978095017595114E-5	0.0	0.011954175527873319	7.558380533430717E-4
30	0.0	3.978095017595114E-5	0.0	0.021223136918869935	7.558380533430717E-4
31	0.0	3.978095017595114E-5	0.0	0.0736146483005976	7.558380533430717E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGAGT	800	0.0	18.0375	4
TAGAGTG	825	0.0	17.715153	5
ATACCGT	1330	0.0	16.691729	6
CGTCGTA	1225	0.0	16.008165	10
ACCGTCG	1230	0.0	15.9430895	8
GGTTATC	810	0.0	15.759259	2
TACCGTC	1320	0.0	15.696969	7
CCGTCGT	1255	0.0	15.625499	9
TCGTTAC	1020	0.0	15.416667	23
AAGACGG	1480	0.0	15.375	5
ATTAGAG	840	0.0	15.196428	3
CGCTTCG	835	0.0	15.065868	32
TATACCG	235	0.0	14.957447	5
CGAACGT	360	0.0	14.902778	4
GTCGTAG	1335	0.0	14.827716	11
CGTTACG	930	0.0	14.720429	24
AACGAAC	760	0.0	14.605264	14
CGAGCCG	1210	0.0	14.524793	15
CCGATAA	780	0.0	14.467948	9
CAAGACG	1505	0.0	14.382061	4
>>END_MODULE