FastQCFastQC Report
Thu 9 Feb 2017
SRR2727251.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727251.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4645255
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGT3200.019.0781254
TAACGTA803.385336E-416.18754
TATACTG5900.015.9915265
TCTTATA10750.015.31627937
TCGTTAC8800.014.92613623
TATCTAG9150.014.7595631
TTAGAGT5600.014.5357154
TAATACT6550.014.4045794
TCGAACG4000.014.3375013
AAGACGG10200.013.9656865
TAGATCG1203.3046097E-513.8749995
TAGAGTG6550.013.8396945
CTCTTAC7900.013.5822781
TACGGAT14600.013.5582227
GTATTAG9750.013.4717951
CAAGACG11050.013.2262454
TAGACAG9400.013.1861715
CGTTACG7400.013.024
GTTACGG11100.013.025
CTCTATA2850.012.9824562
Produced by FastQC (version 0.11.5)