##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727251.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4645255
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.48668307767819	31.0	31.0	34.0	30.0	34.0
2	31.70089133965735	31.0	31.0	34.0	30.0	34.0
3	31.872336394880367	31.0	31.0	34.0	30.0	34.0
4	35.593569782498484	37.0	35.0	37.0	33.0	37.0
5	35.38558033950773	37.0	35.0	37.0	33.0	37.0
6	35.394910074904395	37.0	35.0	37.0	33.0	37.0
7	35.290283525877484	37.0	35.0	37.0	32.0	37.0
8	35.249845487492074	37.0	35.0	37.0	32.0	37.0
9	36.77414070917528	39.0	35.0	39.0	32.0	39.0
10	36.57628332567319	38.0	35.0	39.0	32.0	39.0
11	36.626354634998506	39.0	35.0	39.0	32.0	39.0
12	36.459903708192556	38.0	35.0	39.0	32.0	39.0
13	36.44135897814006	38.0	35.0	39.0	32.0	39.0
14	37.41512941700725	39.0	36.0	41.0	32.0	41.0
15	37.33899086271905	39.0	36.0	41.0	32.0	41.0
16	37.24416657427848	39.0	36.0	41.0	31.0	41.0
17	37.568234036667526	39.0	36.0	41.0	32.0	41.0
18	37.59962693113726	39.0	36.0	41.0	32.0	41.0
19	37.672329075583576	40.0	37.0	41.0	32.0	41.0
20	37.632827907187014	39.0	37.0	41.0	32.0	41.0
21	37.58325323367608	39.0	37.0	41.0	32.0	41.0
22	37.515931633462536	39.0	37.0	41.0	32.0	41.0
23	37.391189288854974	39.0	36.0	41.0	31.0	41.0
24	37.26388432927794	39.0	36.0	41.0	31.0	41.0
25	37.176612263481765	39.0	36.0	41.0	31.0	41.0
26	36.87956742955984	39.0	36.0	41.0	30.0	41.0
27	36.68941166846599	39.0	36.0	40.0	30.0	41.0
28	36.47571145179328	39.0	36.0	40.0	30.0	41.0
29	36.30108056500666	39.0	35.0	40.0	29.0	41.0
30	36.15880484494393	39.0	35.0	40.0	28.0	41.0
31	35.99834390146504	39.0	35.0	40.0	27.0	41.0
32	35.84806151653677	38.0	35.0	40.0	27.0	41.0
33	35.674812254655556	38.0	35.0	40.0	26.0	41.0
34	35.54707782457583	38.0	34.0	40.0	26.0	41.0
35	35.38628213090562	38.0	34.0	40.0	25.0	41.0
36	35.28141361453785	38.0	34.0	40.0	25.0	41.0
37	35.2037317649946	38.0	34.0	40.0	25.0	41.0
38	35.08512729656391	38.0	34.0	40.0	24.0	41.0
39	34.93761634183699	38.0	34.0	40.0	24.0	41.0
40	34.84993288850666	38.0	33.0	40.0	24.0	41.0
41	34.631345103767174	38.0	33.0	40.0	23.0	41.0
42	34.45533237680171	38.0	33.0	40.0	23.0	41.0
43	34.179077359585214	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	4.0
11	16.0
12	24.0
13	45.0
14	78.0
15	147.0
16	254.0
17	470.0
18	939.0
19	1853.0
20	3482.0
21	6564.0
22	11191.0
23	17900.0
24	26863.0
25	37163.0
26	50031.0
27	65837.0
28	84703.0
29	105882.0
30	131305.0
31	160964.0
32	195537.0
33	235916.0
34	289190.0
35	353850.0
36	446552.0
37	580400.0
38	891310.0
39	946781.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.841460802474785	19.986287082194625	12.243956467405988	26.928295647924607
2	20.049728163470036	22.190321091091878	32.786983707030075	24.972967038408008
3	19.469673893037086	24.324972471909508	29.423121012732345	26.782232622321057
4	15.181685397249451	15.890796091926063	33.56190349076638	35.3656150200581
5	13.743314414386292	38.52505406054135	33.697288953997145	14.034342571075214
6	35.65987227827105	36.471539237350804	14.25441660360949	13.614171880768655
7	29.644163775723833	31.1585908631496	20.44479797126315	18.75244738986342
8	27.795180243065236	35.06233780492137	18.711566964569222	18.430914987444176
9	28.25834103832836	13.620996048656101	17.179616619539722	40.941046293475814
10	16.344140418556137	27.007257943858843	32.943056947358116	23.70554469022691
11	38.29447468438224	21.386511612387263	19.990549496206345	20.328464207024158
12	21.975392093652555	24.863629660804413	28.83615646503798	24.324821780505054
13	30.973778619257715	19.430472600535385	23.83128590357257	25.76446287663433
14	23.117310029266424	20.068306260904944	23.467086306349167	33.347297403479466
15	25.937736464413685	27.75748586460808	20.584252102414183	25.72052556856405
16	25.833737867996483	25.884951418167574	22.821244474200018	25.460066239635932
17	25.106070603228453	25.895542871166384	23.43001622085332	25.568370304751838
18	25.40146881064656	25.14639131759182	24.277461624819306	25.17467824694231
19	26.221294632910357	24.986507737465434	23.958254175497363	24.833943454126846
20	25.545120773778834	24.328330737494497	24.00481781947385	26.12173066925282
21	25.962794292240147	24.97253649153814	24.200501371829965	24.864167844391748
22	26.132085321473202	24.223341883276593	23.630414261434517	26.01415853381569
23	25.215128125366636	24.367941049522575	24.272338117067846	26.14459270804294
24	25.41081167772275	24.679786147369736	23.954852855225386	25.95454931968213
25	26.020810482955188	24.088171693480767	23.65125272993625	26.2397650936278
26	25.867729543372754	24.653113768781264	23.970072687075305	25.509084000770677
27	26.542073578307328	24.614859679393273	23.312240124600265	25.530826617699137
28	25.543958297230184	25.080496119158152	23.583936726832004	25.791608856779657
29	25.70799665465082	25.006011510670568	24.00927397957701	25.276717855101605
30	26.18456898491041	24.855707598398798	23.644277870644345	25.31544554604645
31	26.259225812145942	24.38421572120368	23.77884529482235	25.577713171828027
32	25.605160534782268	24.697524678408396	23.490615692787586	26.20669909402175
33	25.47718047771328	24.150148915398617	23.89203606691129	26.480634539976815
34	26.154280012615022	24.437431314319667	24.238023531539174	25.170265141526137
35	26.333710420633526	24.33244246010176	23.983376585354304	25.350470533910407
36	25.750685376798476	24.51770677820701	24.225559199656423	25.50604864533809
37	25.863596293422	24.334487557733645	24.48931221213905	25.3126039367053
38	26.012414818992713	23.768103150419083	24.38966213910754	25.82981989148066
39	25.781706278772642	24.142076161588548	24.41691575597034	25.659301803668477
40	26.263423644127183	23.762419931736794	24.57608893376144	25.398067490374586
41	25.096232607251917	24.424514908223554	24.815429938722417	25.663822545802113
42	25.840153016357554	24.21098518811131	24.86354355143044	25.0853182441007
43	25.158597321352648	23.61489304677569	24.758705388616985	26.467804243254676
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	100.0
1	114.5
2	129.0
3	145.0
4	161.0
5	161.0
6	231.0
7	301.0
8	387.0
9	473.0
10	678.5
11	884.0
12	884.0
13	1857.0
14	2830.0
15	4461.0
16	6092.0
17	6004.0
18	5916.0
19	5916.0
20	7879.0
21	9842.0
22	11281.0
23	12720.0
24	16761.5
25	20803.0
26	20803.0
27	27094.0
28	33385.0
29	40748.0
30	48111.0
31	59375.0
32	70639.0
33	70639.0
34	87414.5
35	104190.0
36	128511.5
37	152833.0
38	180193.5
39	207554.0
40	207554.0
41	230915.5
42	254277.0
43	278078.5
44	301880.0
45	335729.0
46	369578.0
47	369578.0
48	406895.0
49	444212.0
50	452617.0
51	461022.0
52	448266.5
53	435511.0
54	435511.0
55	418645.5
56	401780.0
57	378567.0
58	355354.0
59	326094.0
60	296834.0
61	296834.0
62	255723.5
63	214613.0
64	184751.0
65	154889.0
66	129303.5
67	103718.0
68	103718.0
69	84034.0
70	64350.0
71	54644.0
72	44938.0
73	35577.0
74	26216.0
75	26216.0
76	20391.5
77	14567.0
78	12319.5
79	10072.0
80	7778.5
81	5485.0
82	5485.0
83	4489.5
84	3494.0
85	2781.5
86	2069.0
87	1907.5
88	1746.0
89	1746.0
90	1220.5
91	695.0
92	555.5
93	416.0
94	411.0
95	406.0
96	406.0
97	241.5
98	77.0
99	85.0
100	93.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4645255.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.8243766598264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.87794433423804	42.72326994984263
2	9.4281297269125	9.960701517842642
3	3.450373232272825	5.467914457156872
4	1.6790369161177388	3.5477631393102707
5	1.0392499177555905	2.744886454960746
6	0.6740942431192655	2.1365164921651605
7	0.4766791929681636	1.7626196864676642
8	0.3375010488232598	1.4262626020901068
9	0.265574627345499	1.2625932731572505
>10	1.6166533597759456	15.755510676453275
>50	0.0977609003925961	3.526584045738501
>100	0.04805733815824838	4.849573072512443
>500	0.006208514849353969	2.3390516999955384
>1k	0.002736647271191205	2.4967529323069964
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.610937397408754E-5
2	0.0	0.0	0.0	0.0	8.610937397408754E-5
3	0.0	0.0	0.0	0.0	8.610937397408754E-5
4	0.0	0.0	0.0	0.0	1.0763671746760942E-4
5	0.0	2.1527343493521884E-5	0.0	0.0	1.0763671746760942E-4
6	0.0	2.1527343493521884E-5	0.0	0.0	1.7221874794817507E-4
7	0.0	2.1527343493521884E-5	0.0	0.0	1.7221874794817507E-4
8	0.0	2.1527343493521884E-5	0.0	0.0	1.7221874794817507E-4
9	0.0	2.1527343493521884E-5	0.0	2.1527343493521884E-5	2.3680077842874072E-4
10	0.0	2.1527343493521884E-5	0.0	4.305468698704377E-5	2.3680077842874072E-4
11	0.0	2.1527343493521884E-5	0.0	6.458203048056565E-5	2.3680077842874072E-4
12	0.0	2.1527343493521884E-5	0.0	6.458203048056565E-5	2.3680077842874072E-4
13	0.0	2.1527343493521884E-5	0.0	8.610937397408754E-5	2.583281219222626E-4
14	0.0	2.1527343493521884E-5	0.0	1.0763671746760942E-4	3.6596483938987204E-4
15	0.0	2.1527343493521884E-5	0.0	1.506914044546532E-4	3.874921828833939E-4
16	0.0	2.1527343493521884E-5	0.0	1.7221874794817507E-4	4.520742133639596E-4
17	0.0	2.1527343493521884E-5	0.0	2.1527343493521885E-4	4.520742133639596E-4
18	0.0	2.1527343493521884E-5	0.0	2.3680077842874072E-4	5.381835873380471E-4
19	0.0	2.1527343493521884E-5	0.0	2.583281219222626E-4	5.381835873380471E-4
20	0.0	2.1527343493521884E-5	0.0	2.798554654157845E-4	5.381835873380471E-4
21	0.0	2.1527343493521884E-5	0.0	3.2291015240282824E-4	5.59710930831569E-4
22	0.0	2.1527343493521884E-5	0.0	4.7360155685748144E-4	5.59710930831569E-4
23	0.0	2.1527343493521884E-5	0.0	6.673476482991784E-4	7.104023352862221E-4
24	0.0	2.1527343493521884E-5	0.0	0.001097894518169616	7.319296787797441E-4
25	0.0	2.1527343493521884E-5	0.0	0.0011840038921437036	7.319296787797441E-4
26	0.0	2.1527343493521884E-5	0.0	0.0015499687315335756	7.534570222732659E-4
27	0.0	2.1527343493521884E-5	0.0	0.003961031202808027	7.534570222732659E-4
28	0.0	2.1527343493521884E-5	0.0	0.008546355366928188	7.534570222732659E-4
29	0.0	2.1527343493521884E-5	0.0	0.01621008965062198	7.749843657667878E-4
30	0.0	2.1527343493521884E-5	0.0	0.027339726236772794	7.749843657667878E-4
31	0.0	2.1527343493521884E-5	0.0	0.0879391981710369	7.749843657667878E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGT	320	0.0	19.078125	4
TAACGTA	80	3.385336E-4	16.1875	4
TATACTG	590	0.0	15.991526	5
TCTTATA	1075	0.0	15.316279	37
TCGTTAC	880	0.0	14.926136	23
TATCTAG	915	0.0	14.759563	1
TTAGAGT	560	0.0	14.535715	4
TAATACT	655	0.0	14.404579	4
TCGAACG	400	0.0	14.337501	3
AAGACGG	1020	0.0	13.965686	5
TAGATCG	120	3.3046097E-5	13.874999	5
TAGAGTG	655	0.0	13.839694	5
CTCTTAC	790	0.0	13.582278	1
TACGGAT	1460	0.0	13.55822	27
GTATTAG	975	0.0	13.471795	1
CAAGACG	1105	0.0	13.226245	4
TAGACAG	940	0.0	13.186171	5
CGTTACG	740	0.0	13.0	24
GTTACGG	1110	0.0	13.0	25
CTCTATA	285	0.0	12.982456	2
>>END_MODULE