FastQCFastQC Report
Thu 9 Feb 2017
SRR2727250.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727250.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3910374
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT40600.10382638591602746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24800.016.858871
AATTAGA6250.016.5762
TATACCG2150.016.3488375
AAGACGG10550.016.3080565
TTAGAGT6550.016.0992374
TAGAGTG6700.015.7388065
TAGTACT3600.015.4166664
CAAGACG12200.015.1639344
TAAGACT4950.014.5757584
GGTTATC5750.014.4782612
CTTAGCG1803.3378456E-914.3888883
GACGGTA8400.014.0952398
ACGACGG8550.014.0643276
ATTAGAG6450.014.0542643
AGAGTGT9300.013.9247316
TAGGTCG1601.7867023E-713.87521
CGACGGT8450.013.7928987
ACGGACC12150.013.7037038
CGGACCA12350.013.4817829
ACGGTAT8700.013.3965519
Produced by FastQC (version 0.11.5)