##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727250.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3910374
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.420433953376328	31.0	31.0	34.0	30.0	34.0
2	31.645243140425954	31.0	31.0	34.0	30.0	34.0
3	31.814119570148534	31.0	31.0	34.0	30.0	34.0
4	35.54515527159295	37.0	35.0	37.0	33.0	37.0
5	35.33483063256865	37.0	35.0	37.0	33.0	37.0
6	35.35148172527743	37.0	35.0	37.0	33.0	37.0
7	35.24464514135988	37.0	35.0	37.0	32.0	37.0
8	35.20142318867709	37.0	35.0	37.0	32.0	37.0
9	36.72293161728264	39.0	35.0	39.0	32.0	39.0
10	36.50230694046145	38.0	35.0	39.0	32.0	39.0
11	36.56278785609765	38.0	35.0	39.0	32.0	39.0
12	36.3838382211011	38.0	35.0	39.0	32.0	39.0
13	36.37276869169036	38.0	35.0	39.0	32.0	39.0
14	37.34257081291968	39.0	36.0	41.0	32.0	41.0
15	37.254924465025596	39.0	36.0	41.0	31.0	41.0
16	37.171062921347165	39.0	36.0	40.0	31.0	41.0
17	37.4840048547786	39.0	36.0	41.0	32.0	41.0
18	37.50941086453623	39.0	36.0	41.0	32.0	41.0
19	37.573935638892856	39.0	37.0	41.0	32.0	41.0
20	37.530873006009145	39.0	36.0	41.0	32.0	41.0
21	37.476359294532955	39.0	36.0	41.0	32.0	41.0
22	37.396320403112334	39.0	36.0	41.0	31.0	41.0
23	37.265462843196076	39.0	36.0	41.0	31.0	41.0
24	37.12675871924271	39.0	36.0	41.0	31.0	41.0
25	37.0351966333655	39.0	36.0	41.0	31.0	41.0
26	36.72116375569191	39.0	36.0	40.0	30.0	41.0
27	36.51621890898415	39.0	36.0	40.0	30.0	41.0
28	36.28948867806506	39.0	35.0	40.0	29.0	41.0
29	36.09258756323564	39.0	35.0	40.0	27.0	41.0
30	35.9459230753887	39.0	35.0	40.0	27.0	41.0
31	35.7753404150089	38.0	35.0	40.0	27.0	41.0
32	35.61373592398067	38.0	34.0	40.0	26.0	41.0
33	35.432581129068474	38.0	34.0	40.0	25.0	41.0
34	35.29409821157772	38.0	34.0	40.0	25.0	41.0
35	35.11312191621569	38.0	34.0	40.0	25.0	41.0
36	35.00244529039933	38.0	34.0	40.0	24.0	41.0
37	34.905668358064986	38.0	33.0	40.0	24.0	41.0
38	34.77628840617292	38.0	33.0	40.0	24.0	41.0
39	34.629828758067646	38.0	33.0	40.0	23.0	41.0
40	34.52235949809405	38.0	33.0	40.0	23.0	41.0
41	34.29643098077064	38.0	33.0	40.0	22.0	41.0
42	34.101167816684544	38.0	33.0	40.0	21.0	41.0
43	33.81548695853645	38.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	15.0
12	32.0
13	27.0
14	56.0
15	141.0
16	243.0
17	491.0
18	951.0
19	1824.0
20	3403.0
21	6411.0
22	11022.0
23	17348.0
24	25649.0
25	34375.0
26	46023.0
27	59419.0
28	76265.0
29	94569.0
30	116389.0
31	141036.0
32	169978.0
33	203681.0
34	247095.0
35	302104.0
36	379977.0
37	492069.0
38	747219.0
39	732556.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.91743500749544	20.97666361325029	12.685231642804498	26.42066973644976
2	20.17697028468377	22.676884615128888	32.61439954336849	24.531745556818862
3	19.348174880459005	24.54358585649352	29.762830869886102	26.345408393161367
4	15.101829134502223	16.43085802023029	34.013396161083314	34.45391668418417
5	13.623274909254205	38.55119740464723	33.93721418974246	13.888313496356103
6	35.01248218201123	37.38228108104237	14.186008806318782	13.419227930627608
7	29.443833249709616	31.391831062706533	20.56614022085867	18.59819546672518
8	27.52969409064197	35.08815269332294	18.742094745924557	18.64005847011053
9	27.61165556031213	13.904398914272651	17.78998632867342	40.693959196741794
10	16.31207142846183	27.225682249319377	33.048808119121084	23.41343820309771
11	37.95994961095793	21.74584835107844	20.26962126896302	20.024580769000615
12	21.78072992506599	25.409436539829695	28.975080133000063	23.83475340210425
13	30.706244466641806	19.6509336447102	23.99867634144458	25.64414554720341
14	22.955297881992873	20.484639065214736	23.641012343064883	32.91905070972751
15	25.486539139223	28.17428716537088	20.658842351140837	25.680331344265277
16	25.658312990010675	26.187725266176585	22.82566833760658	25.32829340620616
17	24.76962561637327	26.365636637313976	23.669321655677948	25.195416090634808
18	25.210734318507644	25.17685009157692	24.54701263868878	25.065402951226663
19	25.831902523901807	25.212268698595068	24.28683292186374	24.668995855639384
20	25.57399880420645	24.623271329034	24.139634725476387	25.66309514128316
21	26.049784496316718	25.18311547693392	24.063094732115136	24.70400529463422
22	26.021372891697826	24.6812197503359	23.928631890453445	25.368775467512826
23	25.1170604141701	24.580615562603477	24.156947647462875	26.145376375763547
24	25.30847433007687	25.065888838254345	24.119994660357296	25.505642171311493
25	25.8197297752082	24.52466183541523	23.931138044596246	25.724470344780322
26	25.744545150924182	25.111613364859732	23.956711046053396	25.18713043816269
27	26.17015661417552	24.90523924310053	23.587948365041296	25.336655777682648
28	25.468842622214655	25.057910061799717	24.06613791928854	25.40710939669709
29	25.555765254167508	25.216360378828213	24.11840913426695	25.109465232737328
30	25.631921652506897	25.21730657988213	24.098308755121632	25.05246301248934
31	25.95301625880287	24.636876165809205	24.01517092738444	25.39493664800349
32	25.44153065665842	24.957280301065833	23.70131348050084	25.89987556177491
33	25.312182415288152	24.499088833958083	24.03018227923979	26.158546471513976
34	25.94219887918649	24.656618522934124	24.36856423452079	25.032618363358594
35	26.1332292000714	24.51410018581343	24.28097670453005	25.071693909585118
36	25.468638038202997	24.817651713109797	24.373857845822418	25.33985240286479
37	25.740351178685213	24.438966707532323	24.4630309019035	25.357651211878967
38	25.43917794052436	24.090508989677204	24.764971330108065	25.705341739690375
39	25.719048868471404	24.197608719779744	24.501390404089225	25.581952007659623
40	25.743189781846954	24.121733624456382	24.921912840050595	25.21316375364607
41	24.95009428765637	24.41807356534183	25.268938469824114	25.362893677177684
42	25.553054516013045	24.265274881635364	25.24257270532179	24.9390978970298
43	25.07591345482555	23.575545459334577	25.18112078282026	26.167420303019608
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	132.0
1	125.0
2	118.0
3	131.0
4	144.0
5	144.0
6	197.5
7	251.0
8	363.5
9	476.0
10	658.5
11	841.0
12	841.0
13	1859.5
14	2878.0
15	4512.0
16	6146.0
17	6164.5
18	6183.0
19	6183.0
20	8236.5
21	10290.0
22	11870.0
23	13450.0
24	17646.5
25	21843.0
26	21843.0
27	28044.0
28	34245.0
29	41223.5
30	48202.0
31	58412.0
32	68622.0
33	68622.0
34	83396.0
35	98170.0
36	120096.0
37	142022.0
38	165726.0
39	189430.0
40	189430.0
41	208389.5
42	227349.0
43	247016.0
44	266683.0
45	288873.0
46	311063.0
47	311063.0
48	335257.0
49	359451.0
50	363036.5
51	366622.0
52	358579.5
53	350537.0
54	350537.0
55	337657.0
56	324777.0
57	306590.5
58	288404.0
59	264183.0
60	239962.0
61	239962.0
62	206806.5
63	173651.0
64	150059.5
65	126468.0
66	105881.0
67	85294.0
68	85294.0
69	69137.5
70	52981.0
71	45173.5
72	37366.0
73	29925.0
74	22484.0
75	22484.0
76	17409.0
77	12334.0
78	10480.5
79	8627.0
80	6649.5
81	4672.0
82	4672.0
83	3879.5
84	3087.0
85	2477.5
86	1868.0
87	1754.0
88	1640.0
89	1640.0
90	1163.0
91	686.0
92	551.5
93	417.0
94	394.0
95	371.0
96	371.0
97	218.0
98	65.0
99	68.5
100	72.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3910374.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.46780925735503
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.7403640335455	45.06686366063274
2	8.8854905331484	9.679464070351218
3	2.9626016850711165	4.840992704639165
4	1.4531827378304198	3.1660672072091307
5	0.8583030059847856	2.3374942207496874
6	0.5623939123012854	1.8379418605634437
7	0.3947617573325224	1.505126567034027
8	0.29914960746157593	1.3035219006903798
9	0.22805995989658112	1.1179733755398316
>10	1.4448011809665133	14.763042910776969
>50	0.1074272653200127	3.989672881454747
>100	0.05419768219871318	5.519853504207882
>500	0.006867661376470909	2.58190412589192
>1k	0.0023989775661476765	2.2900810102589926
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	4060	0.10382638591602746	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.5573001457149624E-5	0.0
5	0.0	2.5573001457149624E-5	0.0	2.5573001457149624E-5	0.0
6	2.5573001457149624E-5	2.5573001457149624E-5	0.0	2.5573001457149624E-5	2.5573001457149624E-5
7	2.5573001457149624E-5	2.5573001457149624E-5	0.0	2.5573001457149624E-5	2.5573001457149624E-5
8	2.5573001457149624E-5	2.5573001457149624E-5	0.0	2.5573001457149624E-5	2.5573001457149624E-5
9	2.5573001457149624E-5	2.5573001457149624E-5	0.0	2.5573001457149624E-5	7.671900437144886E-5
10	2.5573001457149624E-5	2.5573001457149624E-5	0.0	2.5573001457149624E-5	7.671900437144886E-5
11	2.5573001457149624E-5	2.5573001457149624E-5	0.0	5.114600291429925E-5	7.671900437144886E-5
12	2.5573001457149624E-5	2.5573001457149624E-5	0.0	5.114600291429925E-5	7.671900437144886E-5
13	2.5573001457149624E-5	2.5573001457149624E-5	0.0	5.114600291429925E-5	1.022920058285985E-4
14	2.5573001457149624E-5	2.5573001457149624E-5	0.0	5.114600291429925E-5	1.5343800874289773E-4
15	2.5573001457149624E-5	2.5573001457149624E-5	0.0	5.114600291429925E-5	1.5343800874289773E-4
16	2.5573001457149624E-5	2.5573001457149624E-5	0.0	7.671900437144886E-5	2.04584011657197E-4
17	2.5573001457149624E-5	2.5573001457149624E-5	0.0	1.022920058285985E-4	2.04584011657197E-4
18	2.5573001457149624E-5	2.5573001457149624E-5	0.0	1.278650072857481E-4	3.0687601748579545E-4
19	2.5573001457149624E-5	2.5573001457149624E-5	0.0	1.7901101020004737E-4	3.324490189429451E-4
20	2.5573001457149624E-5	2.5573001457149624E-5	0.0	2.04584011657197E-4	3.324490189429451E-4
21	2.5573001457149624E-5	2.5573001457149624E-5	0.0	2.8130301602864586E-4	3.324490189429451E-4
22	2.5573001457149624E-5	2.5573001457149624E-5	0.0	3.0687601748579545E-4	3.324490189429451E-4
23	2.5573001457149624E-5	2.5573001457149624E-5	0.0	5.626060320572917E-4	3.8359502185724434E-4
24	2.5573001457149624E-5	2.5573001457149624E-5	0.0	8.694820495430872E-4	3.8359502185724434E-4
25	2.5573001457149624E-5	2.5573001457149624E-5	0.0	9.462010539145361E-4	3.8359502185724434E-4
26	2.5573001457149624E-5	2.5573001457149624E-5	0.0	0.0015343800874289774	3.8359502185724434E-4
27	2.5573001457149624E-5	2.5573001457149624E-5	0.0	0.004091680233143939	3.8359502185724434E-4
28	2.5573001457149624E-5	7.671900437144886E-5	0.0	0.008490236483773676	3.8359502185724434E-4
29	2.5573001457149624E-5	7.671900437144886E-5	0.0	0.01421858881017519	4.09168023314394E-4
30	2.5573001457149624E-5	7.671900437144886E-5	0.0	0.02708180854312145	4.09168023314394E-4
31	2.5573001457149624E-5	7.671900437144886E-5	0.0	0.08858487704756629	4.09168023314394E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2480	0.0	16.85887	1
AATTAGA	625	0.0	16.576	2
TATACCG	215	0.0	16.348837	5
AAGACGG	1055	0.0	16.308056	5
TTAGAGT	655	0.0	16.099237	4
TAGAGTG	670	0.0	15.738806	5
TAGTACT	360	0.0	15.416666	4
CAAGACG	1220	0.0	15.163934	4
TAAGACT	495	0.0	14.575758	4
GGTTATC	575	0.0	14.478261	2
CTTAGCG	180	3.3378456E-9	14.388888	3
GACGGTA	840	0.0	14.095239	8
ACGACGG	855	0.0	14.064327	6
ATTAGAG	645	0.0	14.054264	3
AGAGTGT	930	0.0	13.924731	6
TAGGTCG	160	1.7867023E-7	13.875	21
CGACGGT	845	0.0	13.792898	7
ACGGACC	1215	0.0	13.703703	8
CGGACCA	1235	0.0	13.481782	9
ACGGTAT	870	0.0	13.396551	9
>>END_MODULE