FastQCFastQC Report
Thu 9 Feb 2017
SRR2727249.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727249.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5354687
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT64140.1197829116809255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG14450.017.667825
CAAGACG15150.017.095714
GGTATCA30900.016.344661
GTACTAG2850.015.5789471
TAATCCC13500.015.2111115
TTAGAGT8450.014.8875734
TATACTG7350.014.8503415
ATTAGAG8850.014.8418073
AACGTAT750.00410701414.827
TAGAGTG8900.014.7584275
CGCAAGA16700.014.6227542
GTATTAG13950.014.5878131
GACGGAC17100.014.4970767
CACTCTA11500.014.4782619
TATCTAG11500.014.4782611
TTTAATC17950.014.3259063
ATACCGT12800.014.3085946
CGAACGA8350.014.17964116
ATTATAC5750.014.1565223
TCGTTAC10650.014.07042223
Produced by FastQC (version 0.11.5)