##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727249.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5354687
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49406211791651	31.0	31.0	34.0	30.0	34.0
2	31.7034241217087	31.0	31.0	34.0	30.0	34.0
3	31.87623235494437	31.0	31.0	34.0	30.0	34.0
4	35.595399133506774	37.0	35.0	37.0	33.0	37.0
5	35.39433266594294	37.0	35.0	37.0	33.0	37.0
6	35.401493121820195	37.0	35.0	37.0	33.0	37.0
7	35.30040840855871	37.0	35.0	37.0	32.0	37.0
8	35.261946029711915	37.0	35.0	37.0	32.0	37.0
9	36.782815316749605	39.0	37.0	39.0	32.0	39.0
10	36.59032470058474	39.0	35.0	39.0	32.0	39.0
11	36.6444522340895	39.0	35.0	39.0	32.0	39.0
12	36.4753715763405	38.0	35.0	39.0	32.0	39.0
13	36.467417423277965	38.0	35.0	39.0	32.0	39.0
14	37.42883757724775	39.0	36.0	41.0	32.0	41.0
15	37.36498828036074	39.0	36.0	41.0	32.0	41.0
16	37.26214324011843	39.0	36.0	41.0	31.0	41.0
17	37.584565260303734	39.0	36.0	41.0	32.0	41.0
18	37.61371224872714	39.0	36.0	41.0	32.0	41.0
19	37.68638372326898	40.0	37.0	41.0	32.0	41.0
20	37.63896956068581	40.0	37.0	41.0	32.0	41.0
21	37.58453425942543	39.0	37.0	41.0	32.0	41.0
22	37.51531900930904	39.0	37.0	41.0	32.0	41.0
23	37.384694941086195	39.0	36.0	41.0	31.0	41.0
24	37.24250362346109	39.0	36.0	41.0	31.0	41.0
25	37.14746669599923	39.0	36.0	41.0	31.0	41.0
26	36.843047595499044	39.0	36.0	41.0	30.0	41.0
27	36.651044402782084	39.0	36.0	41.0	30.0	41.0
28	36.40931001195775	39.0	35.0	40.0	29.0	41.0
29	36.223888529805755	39.0	35.0	40.0	29.0	41.0
30	36.0797592837826	39.0	35.0	40.0	27.0	41.0
31	35.91823144844881	39.0	35.0	40.0	27.0	41.0
32	35.746210189316386	38.0	35.0	40.0	27.0	41.0
33	35.56940433679877	38.0	34.0	40.0	26.0	41.0
34	35.431554449401055	38.0	34.0	40.0	25.0	41.0
35	35.245044388215405	38.0	34.0	40.0	25.0	41.0
36	35.12456246275459	38.0	34.0	40.0	24.0	41.0
37	35.04751370154782	38.0	34.0	40.0	24.0	41.0
38	34.91485515399873	38.0	34.0	40.0	24.0	41.0
39	34.75113876870861	38.0	33.0	40.0	23.0	41.0
40	34.65288241871094	38.0	33.0	40.0	23.0	41.0
41	34.4184009261419	38.0	33.0	40.0	22.0	41.0
42	34.239184475208354	38.0	33.0	40.0	21.0	41.0
43	33.97084572823771	38.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	10.0
11	22.0
12	48.0
13	62.0
14	107.0
15	196.0
16	354.0
17	688.0
18	1235.0
19	2460.0
20	4664.0
21	8129.0
22	13603.0
23	22062.0
24	32327.0
25	45025.0
26	60315.0
27	78471.0
28	100225.0
29	124395.0
30	152531.0
31	187278.0
32	227718.0
33	272657.0
34	333201.0
35	407341.0
36	514241.0
37	665805.0
38	1020369.0
39	1079145.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.045218889544806	19.833895053062857	12.17115024650367	26.949735810888665
2	20.153278800422882	22.18323872151631	32.70217288144013	24.96130959662068
3	19.503885101033916	24.307769996640328	29.575921057570685	26.612423844755074
4	14.96569267260626	15.79401373040105	33.90730401235404	35.33298958463865
5	13.710269152986907	38.750799066313306	33.592420994915294	13.946510785784492
6	35.576663958136116	36.720278888383206	14.323619662549838	13.379437490930842
7	29.59444688363671	31.45205312654129	20.391500007376713	18.561999982445286
8	27.903610425782123	34.97520956873856	18.77353428874554	18.34764571673377
9	28.50663353432236	13.511956908032161	17.0993001084844	40.88210944916108
10	16.292866417775677	26.9206211306095	32.67457089461999	24.111941556994836
11	38.571199399703474	21.29412979694238	19.936590131225223	20.19808067212892
12	22.118006150499554	24.884573085224215	28.54710275315812	24.45031801111811
13	30.85534224502758	19.215651633792973	23.835622885147163	26.093383236032285
14	23.07352418544725	20.14870336959004	23.292397856307943	33.48537458865476
15	25.93412089259372	27.870928776976132	20.485006873417625	25.709943457012518
16	25.95471966895544	25.794336811843532	22.766876943507622	25.484066575693408
17	25.049213894294848	25.786157062028085	23.20624903005535	25.958380013621714
18	25.412596478561678	25.051454921641547	24.2596812848258	25.27626731497098
19	26.563625474280755	24.736048997821907	23.674997250072693	25.02532827782464
20	25.625251298535286	24.07630922218236	23.698752139947675	26.599687339334682
21	26.37050494267919	24.78710707087081	24.12587327700013	24.71651470944987
22	26.36337100562554	24.000823951054468	23.427102275072286	26.208702768247704
23	25.33251710137306	24.059800320728364	24.27561125421523	26.332071323683344
24	25.383836627612403	24.326146420883237	24.05160189568503	26.23841505581932
25	26.127073347144286	23.82923595720908	23.580108417167988	26.463582278478647
26	25.92775263988353	24.62321327091574	23.855904182634767	25.59312990656597
27	26.804386512227513	24.413060931479283	23.213158864374332	25.569393691918872
28	25.58283985599905	25.186271391773225	23.299849272235708	25.931039479992013
29	25.768098116659292	25.104978124771808	23.884720059267703	25.242203699301193
30	26.529673909978307	24.58819721862361	23.47399577230191	25.408133099096176
31	26.53531382880082	24.28459403882991	23.570584050944525	25.609508081424742
32	25.820538156571992	24.56739301475511	23.157954890734043	26.454113937938857
33	25.599255381313608	23.776385062282817	23.710984414215062	26.913375142188517
34	26.493182514682935	24.3172756876359	24.107889032542893	25.08165276513828
35	26.777736962029714	24.022524565861648	23.812745730982968	25.38699274112567
36	25.876956020025073	24.44557450323427	24.113398224770187	25.56407125197047
37	26.1497450738017	24.383217917312443	24.33494245321902	25.13209455566684
38	26.411366341300624	23.39849182594613	24.29465998666215	25.895481846091094
39	26.000679404790606	24.071416312475407	24.274640142364998	25.653264140368986
40	26.719320849192492	23.444246134274515	24.388503006805067	25.447930009727926
41	25.213985429960704	24.454519937393165	24.651058035698444	25.680436596947683
42	26.263029006177206	24.094648295969492	24.711453722691914	24.930868975161385
43	25.347233180949697	23.51834943853861	24.425367906658224	26.70904947385347
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	74.0
1	100.5
2	127.0
3	139.0
4	151.0
5	151.0
6	229.5
7	308.0
8	370.0
9	432.0
10	646.0
11	860.0
12	860.0
13	1865.0
14	2870.0
15	4494.5
16	6119.0
17	6176.5
18	6234.0
19	6234.0
20	8287.0
21	10340.0
22	11886.0
23	13432.0
24	18100.5
25	22769.0
26	22769.0
27	29616.0
28	36463.0
29	44802.5
30	53142.0
31	65607.0
32	78072.0
33	78072.0
34	97524.0
35	116976.0
36	146034.5
37	175093.0
38	207466.0
39	239839.0
40	239839.0
41	264263.5
42	288688.0
43	314355.0
44	340022.0
45	379768.0
46	419514.0
47	419514.0
48	462764.0
49	506014.0
50	517539.0
51	529064.0
52	518234.5
53	507405.0
54	507405.0
55	486522.5
56	465640.0
57	440984.5
58	416329.0
59	382917.5
60	349506.0
61	349506.0
62	299335.0
63	249164.0
64	215431.0
65	181698.0
66	152348.5
67	122999.0
68	122999.0
69	99644.5
70	76290.0
71	65795.0
72	55300.0
73	44189.5
74	33079.0
75	33079.0
76	25608.5
77	18138.0
78	15455.5
79	12773.0
80	9828.0
81	6883.0
82	6883.0
83	5797.0
84	4711.0
85	3884.0
86	3057.0
87	2836.5
88	2616.0
89	2616.0
90	1821.5
91	1027.0
92	838.0
93	649.0
94	614.5
95	580.0
96	580.0
97	347.5
98	115.0
99	120.0
100	125.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5354687.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.66623408213983
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.16780040350017	41.93634575579643
2	9.625667194135904	9.946439488979994
3	3.301002731560369	5.11651139503743
4	1.6323085582850758	3.373409442665477
5	0.9610270419390983	2.482632405404593
6	0.6334141242377596	1.9635673448281012
7	0.4317391417534129	1.5614434892177786
8	0.31800478740164906	1.3144087828107769
9	0.26036487325285923	1.2106865239424005
>10	1.505372715149653	14.296303247925765
>50	0.09862999110091099	3.450140846470108
>100	0.05208042095741151	5.339488763990414
>500	0.006511047481488009	2.3776836410693747
>1k	0.006004624372340496	5.411624214409453
>5k	7.234487195526138E-5	0.21931465745194312
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	6414	0.1197829116809255	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.602568366741137E-5
2	0.0	0.0	0.0	0.0	5.602568366741137E-5
3	0.0	0.0	0.0	0.0	5.602568366741137E-5
4	0.0	0.0	0.0	0.0	5.602568366741137E-5
5	0.0	0.0	0.0	1.8675227889137123E-5	5.602568366741137E-5
6	0.0	0.0	0.0	1.8675227889137123E-5	1.6807705100223413E-4
7	0.0	0.0	0.0	3.7350455778274246E-5	1.6807705100223413E-4
8	0.0	0.0	0.0	3.7350455778274246E-5	1.8675227889137125E-4
9	0.0	0.0	0.0	1.1205136733482274E-4	2.6145319044791973E-4
10	0.0	0.0	0.0	1.3072659522395986E-4	2.6145319044791973E-4
11	0.0	0.0	0.0	1.4940182311309698E-4	2.801284183370569E-4
12	0.0	0.0	0.0	2.0542750678050837E-4	3.174788741153311E-4
13	0.0	0.0	0.0	2.427779625587826E-4	3.174788741153311E-4
14	0.0	0.0	0.0	2.427779625587826E-4	3.921797856718796E-4
15	1.8675227889137123E-5	0.0	0.0	2.427779625587826E-4	3.921797856718796E-4
16	1.8675227889137123E-5	0.0	0.0	2.427779625587826E-4	4.2953024145015383E-4
17	1.8675227889137123E-5	0.0	0.0	2.427779625587826E-4	4.2953024145015383E-4
18	1.8675227889137123E-5	0.0	0.0	2.6145319044791973E-4	5.042311530067023E-4
19	1.8675227889137123E-5	0.0	0.0	2.6145319044791973E-4	5.229063808958395E-4
20	1.8675227889137123E-5	0.0	0.0	3.735045577827425E-4	5.229063808958395E-4
21	1.8675227889137123E-5	1.8675227889137123E-5	0.0	5.042311530067023E-4	5.789320645632509E-4
22	1.8675227889137123E-5	1.8675227889137123E-5	1.8675227889137123E-5	5.976072924523879E-4	5.789320645632509E-4
23	3.7350455778274246E-5	1.8675227889137123E-5	1.8675227889137123E-5	7.283338876763479E-4	6.723082040089365E-4
24	3.7350455778274246E-5	1.8675227889137123E-5	1.8675227889137123E-5	9.150861665677191E-4	7.096586597872107E-4
25	3.7350455778274246E-5	1.8675227889137123E-5	1.8675227889137123E-5	9.711118502351304E-4	7.096586597872107E-4
26	3.7350455778274246E-5	1.8675227889137123E-5	1.8675227889137123E-5	0.001213889812793913	7.47009115565485E-4
27	3.7350455778274246E-5	1.8675227889137123E-5	1.8675227889137123E-5	0.002651882360257472	7.843595713437592E-4
28	3.7350455778274246E-5	1.8675227889137123E-5	1.8675227889137123E-5	0.005621243594630274	7.843595713437592E-4
29	3.7350455778274246E-5	1.8675227889137123E-5	1.8675227889137123E-5	0.010925008315145217	8.030347992328963E-4
30	3.7350455778274246E-5	1.8675227889137123E-5	1.8675227889137123E-5	0.019963818613487585	8.030347992328963E-4
31	3.7350455778274246E-5	1.8675227889137123E-5	1.8675227889137123E-5	0.07272133740029996	8.217100271220335E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	1445	0.0	17.66782	5
CAAGACG	1515	0.0	17.09571	4
GGTATCA	3090	0.0	16.34466	1
GTACTAG	285	0.0	15.578947	1
TAATCCC	1350	0.0	15.211111	5
TTAGAGT	845	0.0	14.887573	4
TATACTG	735	0.0	14.850341	5
ATTAGAG	885	0.0	14.841807	3
AACGTAT	75	0.004107014	14.8	27
TAGAGTG	890	0.0	14.758427	5
CGCAAGA	1670	0.0	14.622754	2
GTATTAG	1395	0.0	14.587813	1
GACGGAC	1710	0.0	14.497076	7
CACTCTA	1150	0.0	14.478261	9
TATCTAG	1150	0.0	14.478261	1
TTTAATC	1795	0.0	14.325906	3
ATACCGT	1280	0.0	14.308594	6
CGAACGA	835	0.0	14.179641	16
ATTATAC	575	0.0	14.156522	3
TCGTTAC	1065	0.0	14.070422	23
>>END_MODULE