##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727248.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4315260
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.975981980228305	31.0	30.0	33.0	26.0	34.0
2	30.31546511681799	31.0	30.0	34.0	26.0	34.0
3	31.11900835639104	31.0	31.0	34.0	28.0	34.0
4	35.11403971023762	35.0	35.0	37.0	33.0	37.0
5	34.961963589679414	35.0	35.0	37.0	32.0	37.0
6	34.99714130782386	35.0	35.0	37.0	32.0	37.0
7	34.88105305358194	35.0	35.0	37.0	32.0	37.0
8	34.87129025829266	35.0	35.0	37.0	32.0	37.0
9	36.282861287616505	38.0	35.0	39.0	32.0	39.0
10	36.06857524227972	37.0	35.0	39.0	30.0	39.0
11	36.135006465427345	37.0	35.0	39.0	31.0	39.0
12	35.9165127941306	37.0	35.0	39.0	30.0	39.0
13	35.93709764880911	37.0	35.0	39.0	30.0	39.0
14	36.7472778001789	38.0	36.0	40.0	31.0	41.0
15	36.72254927860662	38.0	36.0	40.0	31.0	41.0
16	36.53489268317552	38.0	35.0	40.0	30.0	41.0
17	37.03766702353972	39.0	36.0	40.0	31.0	41.0
18	37.198781765177536	39.0	36.0	40.0	31.0	41.0
19	37.33365011609961	39.0	36.0	41.0	32.0	41.0
20	37.33815691290907	39.0	36.0	41.0	31.0	41.0
21	37.287193355672656	39.0	36.0	41.0	31.0	41.0
22	37.2333041346292	39.0	36.0	41.0	31.0	41.0
23	37.114022793528086	39.0	36.0	40.0	31.0	41.0
24	37.01029625097908	39.0	36.0	40.0	31.0	41.0
25	36.88823917909929	39.0	36.0	40.0	30.0	41.0
26	36.608675491163915	39.0	36.0	40.0	30.0	41.0
27	36.446550613404526	39.0	35.0	40.0	30.0	41.0
28	36.267668228565604	39.0	35.0	40.0	29.0	41.0
29	36.08078702094428	38.0	35.0	40.0	29.0	41.0
30	35.94601646250747	38.0	35.0	40.0	28.0	41.0
31	35.75018330297595	38.0	34.0	40.0	27.0	41.0
32	35.57934492939012	38.0	34.0	40.0	27.0	41.0
33	35.43419052386183	38.0	34.0	40.0	26.0	41.0
34	35.28783526369211	38.0	34.0	40.0	26.0	41.0
35	35.095962699814145	38.0	34.0	40.0	25.0	41.0
36	34.952457789333664	38.0	33.0	40.0	25.0	41.0
37	34.80552434847495	38.0	33.0	40.0	24.0	41.0
38	34.69421124103762	38.0	33.0	40.0	24.0	41.0
39	34.570566084083	38.0	33.0	40.0	24.0	41.0
40	34.46239600858349	38.0	33.0	40.0	23.0	41.0
41	34.32347784374522	38.0	33.0	40.0	23.0	41.0
42	34.16320777890556	38.0	33.0	40.0	22.0	41.0
43	33.827955673586295	37.0	32.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	11.0
12	9.0
13	17.0
14	47.0
15	97.0
16	197.0
17	419.0
18	870.0
19	1826.0
20	3661.0
21	6683.0
22	11934.0
23	18890.0
24	27766.0
25	38776.0
26	51930.0
27	68353.0
28	89215.0
29	113508.0
30	142657.0
31	176683.0
32	215125.0
33	259448.0
34	313602.0
35	376071.0
36	462222.0
37	578438.0
38	685056.0
39	671746.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.89766549408378	28.23266732479619	14.411391202384097	22.458275978735927
2	20.128126694567648	25.541311531634246	30.350060019558494	23.980501754239604
3	19.467865203950634	25.106413055065048	30.90680515194913	24.518916589035193
4	15.836473352706443	17.284381474117435	32.437257546474605	34.441887626701515
5	13.44176248939809	39.796489666903035	33.076871382025644	13.684876461673227
6	34.42471600784194	38.19517248091656	12.55778330853761	14.822328202703893
7	28.841089528788533	31.979903876012106	19.678211741586836	19.50079485361253
8	28.667635322089513	36.06331947553566	17.6351135273425	17.633931675032326
9	27.130138160852418	14.757627582115562	17.847059041633646	40.26517521539837
10	16.548991254292904	29.952378303972417	32.20867340554219	21.28995703619249
11	36.23053999063788	23.747445113388302	19.17108123264879	20.850933663325037
12	21.2353369206027	27.268090451096803	28.52724980650065	22.969322821799846
13	31.911101532700233	20.537209808910703	23.019655826068416	24.53203283232065
14	21.991930961286226	22.166520673146	25.103493184651676	30.738055180916096
15	25.393556819287827	29.64359042097116	21.428048367885133	23.53480439185588
16	23.898003828274543	27.18174571173	24.051667802171828	24.86858265782363
17	23.98849663751431	27.366554970036567	25.2108331827051	23.434115209744025
18	23.695304570292404	26.407771490014508	25.919342055866856	23.977581883826236
19	24.32738699406293	26.893976260990065	26.493050244944683	22.285586500002317
20	23.99303865815733	26.5943882871484	26.057966379777813	23.354606674916457
21	23.465654444923363	27.11440330362481	26.199325185504463	23.22061706594736
22	24.09254598795901	26.745456820678243	25.33379217011258	23.828205021250167
23	23.75300677131853	26.596497082446945	25.427158502616297	24.22333764361823
24	23.610744196178214	26.655357962208537	25.722482538711454	24.011415302901796
25	24.111548319220628	26.010089774428423	25.56643168661911	24.311930219731835
26	23.98949310122681	26.54567743310948	25.9691188943424	23.49571057132131
27	24.423835412002983	26.92658148060604	25.533362995508963	23.11622011188202
28	23.593387188720957	27.48782228649027	25.563581336929875	23.3552091878589
29	23.88247753321932	27.40284478803131	25.761275102774807	22.953402575974565
30	23.811589568183607	27.29091642218545	25.61706594735891	23.28042806227203
31	23.717806111335122	26.739292649805574	25.96302424419386	23.579876994665444
32	23.176981224769772	27.1004064645004	25.65609024716935	24.06652206356048
33	23.332035613149614	27.01598513183447	25.666935480133297	23.985043774882627
34	23.83921246923708	26.92287370865255	25.8181430551114	23.419770766998976
35	23.64687179914999	27.188744131292204	25.81306804225006	23.35131602730774
36	23.678364687179915	27.198847809865455	25.837006344924756	23.285781158029874
37	23.26967552360692	27.022265170580685	26.225256415604157	23.482802890208237
38	23.56203334213929	27.06641083040188	26.01903477426621	23.352521053192625
39	23.026538377757074	27.38115432210342	26.157149279533563	23.435158020605943
40	23.727678054161277	26.936013125512716	26.407053109198518	22.929255711127485
41	23.547294948624184	26.761029462882885	26.230910767833226	23.460764820659705
42	22.951479169273696	27.101681011109413	26.374725972479062	23.57211384713783
43	23.334538359218215	26.411525609117408	26.315587009820963	23.93834902184341
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1001.0
1	1014.5
2	1028.0
3	1034.5
4	1041.0
5	1041.0
6	1246.0
7	1451.0
8	2060.0
9	2669.0
10	4261.5
11	5854.0
12	5854.0
13	16721.0
14	27588.0
15	48310.5
16	69033.0
17	51780.5
18	34528.0
19	34528.0
20	41057.0
21	47586.0
22	41198.0
23	34810.0
24	40492.0
25	46174.0
26	46174.0
27	56821.0
28	67468.0
29	77236.5
30	87005.0
31	100785.5
32	114566.0
33	114566.0
34	127220.5
35	139875.0
36	157588.0
37	175301.0
38	194002.5
39	212704.0
40	212704.0
41	232426.5
42	252149.0
43	269670.5
44	287192.0
45	306254.0
46	325316.0
47	325316.0
48	344970.5
49	364625.0
50	374825.0
51	385025.0
52	377774.5
53	370524.0
54	370524.0
55	347611.0
56	324698.0
57	292894.5
58	261091.0
59	236402.5
60	211714.0
61	211714.0
62	184013.0
63	156312.0
64	131911.5
65	107511.0
66	89198.5
67	70886.0
68	70886.0
69	58450.5
70	46015.0
71	38122.0
72	30229.0
73	24976.0
74	19723.0
75	19723.0
76	16149.0
77	12575.0
78	10483.5
79	8392.0
80	6614.0
81	4836.0
82	4836.0
83	3866.5
84	2897.0
85	2316.0
86	1735.0
87	1404.5
88	1074.0
89	1074.0
90	799.0
91	524.0
92	405.0
93	286.0
94	216.0
95	146.0
96	146.0
97	108.5
98	71.0
99	51.5
100	32.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4315260.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.01459543065314
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.36789342635056	48.609079070312326
2	9.275809121204247	10.948162451596616
3	3.156635371431851	5.588626781014206
4	1.5035575457636559	3.5492736107979157
5	0.886240172927351	2.615055262984985
6	0.5735583436343628	2.030898816327446
7	0.38996792758151744	1.6109659634007443
8	0.29797097802345685	1.4067709374504274
9	0.21331227335780992	1.1329683761343639
>10	1.2365995993384387	13.255097541949018
>50	0.07040213289574392	2.8039194339476343
>100	0.025031379412509564	2.6710396118310715
>500	0.0015903834367196291	0.6201054623764463
>1k	0.001272306348298091	1.5092952084195956
>5k	3.975957338431251E-5	0.1625347911933672
>10k+	1.1927872015293756E-4	1.4862066802638774
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	37208	0.8622423677831694	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14853	0.3441971051570473	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11279	0.26137474914605374	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6927	0.16052335201123455	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.3173574709287504E-5
2	0.0	0.0	0.0	0.0	2.3173574709287504E-5
3	2.3173574709287504E-5	0.0	0.0	0.0	2.3173574709287504E-5
4	2.3173574709287504E-5	0.0	0.0	0.0	2.3173574709287504E-5
5	2.3173574709287504E-5	0.0	0.0	0.0	2.3173574709287504E-5
6	4.634714941857501E-5	0.0	0.0	0.0	6.952072412786252E-5
7	4.634714941857501E-5	0.0	0.0	0.0	6.952072412786252E-5
8	4.634714941857501E-5	0.0	0.0	0.0	6.952072412786252E-5
9	4.634714941857501E-5	0.0	0.0	2.3173574709287504E-5	1.1586787354643753E-4
10	6.952072412786252E-5	0.0	0.0	4.634714941857501E-5	1.1586787354643753E-4
11	6.952072412786252E-5	0.0	0.0	6.952072412786252E-5	1.1586787354643753E-4
12	6.952072412786252E-5	0.0	0.0	6.952072412786252E-5	1.1586787354643753E-4
13	6.952072412786252E-5	0.0	0.0	6.952072412786252E-5	1.1586787354643753E-4
14	6.952072412786252E-5	0.0	0.0	9.269429883715002E-5	1.6221502296501255E-4
15	6.952072412786252E-5	0.0	0.0	9.269429883715002E-5	1.6221502296501255E-4
16	6.952072412786252E-5	0.0	0.0	1.1586787354643753E-4	2.3173574709287506E-4
17	6.952072412786252E-5	0.0	0.0	3.244300459300251E-4	2.3173574709287506E-4
18	6.952072412786252E-5	0.0	0.0	4.402979194764626E-4	3.012564712207376E-4
19	6.952072412786252E-5	0.0	0.0	5.098186436043251E-4	3.012564712207376E-4
20	6.952072412786252E-5	0.0	0.0	7.415543906972001E-4	3.012564712207376E-4
21	6.952072412786252E-5	0.0	0.0	0.0012977201837201004	3.244300459300251E-4
22	6.952072412786252E-5	0.0	0.0	0.002155142447963738	3.4760362063931255E-4
23	6.952072412786252E-5	0.0	0.0	0.002433225344475188	5.561657930229001E-4
24	6.952072412786252E-5	0.0	0.0	0.0030820854363352384	5.793393677321876E-4
25	6.952072412786252E-5	0.0	0.0	0.003522383355811701	5.793393677321876E-4
26	6.952072412786252E-5	2.3173574709287504E-5	0.0	0.004263937746508901	6.025129424414752E-4
27	9.269429883715002E-5	2.3173574709287504E-5	0.0	0.006233691596798339	6.256865171507626E-4
28	1.1586787354643753E-4	2.3173574709287504E-5	0.0	0.010845232963946553	6.256865171507626E-4
29	1.1586787354643753E-4	4.634714941857501E-5	0.0	0.023938302674693993	6.488600918600502E-4
30	1.1586787354643753E-4	4.634714941857501E-5	0.0	0.0415502194537525	6.488600918600502E-4
31	1.3904144825572503E-4	4.634714941857501E-5	0.0	0.09074771856156987	6.488600918600502E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAC	145	2.9867806E-9	16.586206	3
AAGTACG	930	0.0	16.112904	1
ATACGGG	495	0.0	15.323233	1
TATACTG	580	0.0	15.310345	5
TATAGCG	85	5.368404E-4	15.235294	5
GAGTACT	11180	0.0	14.727192	11
GTACTTT	11400	0.0	14.524123	13
AGTACTT	11525	0.0	14.350542	12
TACTTTT	11665	0.0	14.162452	14
TATATCG	95	0.0012460466	13.631579	5
ACTTTTT	12605	0.0	13.35581	15
TAATCCC	1210	0.0	13.301653	5
AATCAAC	1445	0.0	13.1868515	1
ACGCTAC	225	4.0745363E-10	13.155556	1
CGGGAAT	1335	0.0	13.026217	6
TAATACC	470	0.0	12.989362	4
TTATACT	610	0.0	12.737705	4
TTAATCC	1300	0.0	12.523078	4
ACGACTA	120	5.1265163E-4	12.333334	8
TAGTACT	435	0.0	12.333334	4
>>END_MODULE